| Gene Symbol | RAG2 |
|---|---|
| Entrez Gene | 5897 |
| Alt Symbol | RAG-2 |
| Species | Human |
| Gene Type | protein-coding |
| Description | recombination activating gene 2 |
| Other Description | V(D)J recombination-activating protein 2 |
| Swissprots | P55895 Q8TBL4 A8K9E9 |
| Accessions | AAG38705 CCO13799 EAW68117 P55895 AF080577 AAC35287 AK292664 BAF85353 AW058148 BC022397 AAH22397 BM457214 BM463455 DB124002 DB142398 DB143931 DB146077 DB151960 DB165259 DQ892012 ABM82938 DQ895201 ABM86127 S78372 NM_000536 NP_000527 NM_001243785 NP_001230714 NM_001243786 NP_001230715 NR_033666 |
| Function | Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T- lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process le |
| Subcellular Location | Nucleus {ECO:0000250}. |
| Tissue Specificity | Cells of the B- and T-lymphocyte lineages. |
| Top Pathways | Primary immunodeficiency |
RAG-2 CRISPR/Cas9 KO Plasmid (h) - sc-418602 from Santa Cruz Biotechnology
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RAG-2 HDR Plasmid (h) - sc-418602-HDR from Santa Cruz Biotechnology
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RAG-2 Double Nickase Plasmid (h) - sc-418602-NIC from Santa Cruz Biotechnology
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RAG-2 Double Nickase Plasmid (h2) - sc-418602-NIC-2 from Santa Cruz Biotechnology
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RAG-2 CRISPR Activation Plasmid (h) - sc-418602-ACT from Santa Cruz Biotechnology
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RAG-2 CRISPR Activation Plasmid (h2) - sc-418602-ACT-2 from Santa Cruz Biotechnology
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RAG-2 Lentiviral Activation Particles (h) - sc-418602-LAC from Santa Cruz Biotechnology
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RAG-2 Lentiviral Activation Particles (h2) - sc-418602-LAC-2 from Santa Cruz Biotechnology
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RAG-2 siRNA (h) - sc-36371 from Santa Cruz Biotechnology
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RAG-2 shRNA Plasmid (h) - sc-36371-SH from Santa Cruz Biotechnology
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RAG-2 shRNA (h) Lentiviral Particles - sc-36371-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU000681 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN17916 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND17916 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR05533 from Sigma-Aldrich
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RAG2 siRNA (Human) - CRH3985 from Cohesion Biosciences
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RAG2 RNAi - H00005897-R01 from Novus Biologicals
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RAG2 - MBS8232565 from MyBioSource
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shRNA set against Human RAG2 (NM_000536.3) - SHH389228 from Creative biogene
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RAG2 shRNA Plasmid (Human) - CHH3985 from Cohesion Biosciences
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RAG2 miRNA 3'UTR clone - MiUTR4H-TG12284 from Creative biogene
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