NEK9

Gene Symbol NEK9
Entrez Gene 91754
Alt Symbol NERCC, NERCC1, Nek8
Species Human
Gene Type protein-coding
Description NIMA-related kinase 9
Other Description NIMA (never in mitosis gene a)- related kinase 9|NIMA-related kinase Nek8|nercc1 kinase|never in mitosis A-related kinase 9|nimA-related kinase 8|nimA-related protein kinase 9|serine/threonine-protein kinase Nek9
Swissprots Q8NCN0 Q8TD19 Q9Y6S5 Q9UPI4 Q8TCY4 Q52LK6 Q9Y6S6 Q9Y6S4
Accessions AAD31936 AAD31938 AAD31939 AAD31940 EAW81220 EAW81221 EAW81222 Q8TD19 AB082526 BAC02704 AK128693 BAG54712 AL117502 AL832633 AL833036 AY048580 AAL05428 AY080896 AAL87410 BC001714 AAH01714 BC009336 AAH09336 BC093881 AAH93881 BC112101 AAI12102 BM976692 DB455229 XM_005268208 XP_005268265 XM_005268209 XP_005268266 NM_033116 NP_149107
Function Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively. {ECO:0000269|PubMed:12840024, ECO:0000269|PubMed:14660563, ECO:0000269|PubMed:19941817}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:14660563}. Nucleus {ECO:0000269|PubMed:14660563}.
Tissue Specificity Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts.

Nek9 (N-20) - sc-50765 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Nek9 (N-20): sc-50765. Western blot analysis of Nek9 expression in SK-N-MC whole cell lysate (A) and SK-N-MC nuclear extract (B).

Nek9 (G-20) - sc-50764 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Nek9 (C-20) - sc-50763 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Nek9 (39-7) - sc-100401 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Nek9 (39-7): sc-100401. Western blot analysis of Nek9 expression in non-transfected 293T: sc-117752 (A), mouse Nek9 transfected 293T: sc-122002 (B) and SK-N-MC (C) whole cell lysates.

Anti-NEK9 (phospho T210) antibody - ab63553 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NEK9 (phospho T210) antibody (ab63553) at 1/500 dilutionLane 1 : extracts from HepG2 cells at 30 µg/mlLane 2 : extracts from HepG2 cells at 30 µg with immunizing peptide at 10 µg

Anti-NEK9 antibody - ab71812 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab71812 at 1/50 dilution, staining NEK9 in human hepatocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.

Anti-NEK9 antibody - ab173312 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-NEK9 antibody (ab173312) at 1/100 dilution + mouse heart tissue lysates at 35 µg

Anti-NEK9 antibody - ab57925 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-NEK9 antibody - ab173383 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-NEK9 antibody (ab173383) at 1 µg/mlLane 1 : NEK9-transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.developed using the ECL technique

Anti-NEK9 antibody - ab63257 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NEK9 antibody (ab63257) at 1/500 dilutionLane 1 : extracts from A549 cells, without immunising peptideLane 2 : extracts from A549 cells, with immunising peptide

Anti-NEK9 antibody - ab89853 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-NEK9 antibody (ab89853) at 1 µg/ml + THP-1 cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-NEK9 antibody [EP7361] - ab138488 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP7361
All lanes : Anti-NEK9 antibody [EP7361] (ab138488) at 1/1000 dilutionLane 1 : NIH3T3 cell lysateLane 2 : HepG2 cell lysateLane 3 : HeLa cell lysateLane 4 : THP1 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-NEK9 Antibody (aa503-530, Azide-free, HRP) - LS-C215950 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEK9 Antibody (aa503-530, FITC) - LS-C215949 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEK9 Antibody (aa503-530, PE) - LS-C215953 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEK9 Antibody (C-Terminus, AP) - LS-C220154 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEK9 Antibody (C-Terminus, APC) - LS-C220155 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEK9 Antibody (C-Terminus, Azide-free, HRP) - LS-C220158 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEK9 Antibody (C-Terminus, Biotin) - LS-C220156 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEK9 Antibody (C-Terminus, FITC) - LS-C220157 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEK9 Antibody (C-Terminus, PE) - LS-C220160 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEK9 Antibody (aa675-725) - LS-C287965 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NEK9 Antibody (aa929-979) - LS-C287967 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-NEK9 Antibody (aa502-530) - LS-C323293 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NEK9 Antibody (aa503-530) - LS-C164500 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Mouse Nek9 Antibody western blot of mouse heart tissue lysates (35 ug/lane). The Nek9 antibody detected the Nek9 protein (arrow).
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