Gene Symbol | DNMT3A |
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Entrez Gene | 1788 |
Alt Symbol | DNMT3A2, M.HsaIIIA, TBRS |
Species | Human |
Gene Type | protein-coding |
Description | DNA (cytosine-5-)-methyltransferase 3 alpha |
Other Description | DNA (cytosine-5)-methyltransferase 3A|DNA MTase HsaIIIA|DNA cytosine methyltransferase 3A2 |
Swissprots | Q86TE8 Q8WXU9 Q8IZV0 Q86XF5 E9PEB8 Q9Y6K1 |
Accessions | AAY14761 EAX00727 EAX00728 Q9Y6K1 AB208833 BAD92070 AF067972 AAD33084 AF331856 AAL57039 AF480163 AAN40037 AI376254 AK025230 BC018214 AAH18214 BC023612 AAH23612 BC032392 AAH32392 BC043617 AAH43617 BC051864 AAH51864 BM470515 CB852889 EU446951 ABZ92480 W76111 XM_005264175 XP_005264232 XM_005264177 XP_005264234 XM_006711957 XP_006712020 XM_006711958 XP_006712021 XM_011532662 XP_011530964 XM_011532663 XP_011530965 XM_011532664 XP_011530966 XM_011532665 XP_011530967 XM_011532666 XP_011530968 XM_011532667 XP_011530969 XM_011532668 XP_011530970 NM_022552 NP_072046 NM_153759 NP_715640 NM_175629 NP_783328 NM_175630 NP_783329 |
Function | Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. {ECO:0000269|PubMed:16357870}. |
Subcellular Location | Nucleus {ECO:0000269|PubMed:12145218}. Cytoplasm {ECO:0000269|PubMed:12145218}. Note=Accumulates in the major satellite repeats at pericentric heterochromatin. {ECO:0000250}. |
Tissue Specificity | Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. {ECO:0000269|PubMed:10325416}. |
Top Pathways | Cysteine and methionine metabolism, MicroRNAs in cancer |
Dnmt3a CRISPR/Cas9 KO Plasmid (h) - sc-400323 from Santa Cruz Biotechnology
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Dnmt3a HDR Plasmid (h) - sc-400323-HDR from Santa Cruz Biotechnology
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Dnmt3a Double Nickase Plasmid (h) - sc-400323-NIC from Santa Cruz Biotechnology
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Dnmt3a Double Nickase Plasmid (h2) - sc-400323-NIC-2 from Santa Cruz Biotechnology
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Dnmt3a CRISPR Activation Plasmid (h) - sc-400323-ACT from Santa Cruz Biotechnology
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Dnmt3a CRISPR Activation Plasmid (h2) - sc-400323-ACT-2 from Santa Cruz Biotechnology
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Dnmt3a Lentiviral Activation Particles (h) - sc-400323-LAC from Santa Cruz Biotechnology
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Dnmt3a Lentiviral Activation Particles (h2) - sc-400323-LAC-2 from Santa Cruz Biotechnology
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Dnmt3a siRNA (h) - sc-37757 from Santa Cruz Biotechnology
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Dnmt3a shRNA Plasmid (h) - sc-37757-SH from Santa Cruz Biotechnology
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Dnmt3a shRNA (h) Lentiviral Particles - sc-37757-V from Santa Cruz Biotechnology
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DNMT3A RNAi - H00001788-R02 from Novus Biologicals
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DNMT3A RNAi - H00001788-R03 from Novus Biologicals
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DNMT3A RNAi - H00001788-R04 from Novus Biologicals
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MISSION® esiRNA - EHU123831 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN1553 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND1553 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR08644 from Sigma-Aldrich
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DNMT3A siRNA (Human) - CRH1243 from Cohesion Biosciences
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DNMT3A RNAi - H00001788-R01 from Novus Biologicals
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DNMT3A - MBS8225897 from MyBioSource
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shRNA set against Human DNMT3A (NM_022552.4) - SHH280017 from Creative biogene
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shRNA set against Human DNMT3A(NM_175630.1) - SHH012395 from Creative biogene
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Human DNMT3A siRNA - orb261745 from Biorbyt
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DNMT3A shRNA Plasmid (Human) - CHH1243 from Cohesion Biosciences
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