PRF1

Gene Symbol PRF1
Entrez Gene 5551
Alt Symbol FLH2, HPLH2, P1, PFN1, PFP
Species Human
Gene Type protein-coding
Description perforin 1 (pore forming protein)
Other Description cytolysin|lymphocyte pore forming protein|lymphocyte pore-forming protein|perforin-1
Swissprots Q59F57 Q86WX7 P14222 B2R6X4
Accessions AAA60167 AGS13731 AGS13732 AHC72295 AHC72296 EAW54407 P14222 AB209604 BAD92841 AK312754 BAG35621 BC047695 AAH47695 BC063043 AAH63043 BQ051559 BQ654088 DA816891 DA941244 FJ555237 ACU25794 L40557 AAA63618 M28393 AAA60065 X13224 CAA31612 NM_001083116 NP_001076585 NM_005041 NP_005032
Function Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. {ECO:0000269|PubMed:20038786, ECO:0000269|PubMed:20225066, ECO:0000269|PubMed:20889983, ECO:0000269|PubMed:21037563, ECO:0000269|PubMed:9058810, ECO:0000269|PubMed:9164947}.
Subcellular Location Cytoplasmic granule lumen. Secreted. Cell membrane; Multi-pass membrane protein. Endosome lumen. Note=Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.
Top Pathways Natural killer cell mediated cytotoxicity, Autoimmune thyroid disease, Allograft rejection, Graft-versus-host disease, Type I diabetes mellitus

SimpleChIP ® Human PRF1 Promoter Primers - 9014 from Cell Signaling Technology

Category ChIP Kit
Prices $102.00
Sizes 500 µl (250 PCR reactions)
SimpleChIP ® Human PRF1 Promoter Primers were tested on DNA isolated from cross-linked cells using the SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. Real-time PCR was performed in duplicate on a serial dilution of 2% total input DNA (20 ng, 4 ng, 0.8 ng, and 0.16 ng) using a real-time PCR detection system and SYBR ® Green reaction mix. The PCR amplification efficiency (E) and correlation coefficient (R 2 ) were calculated based on the corresponding threshold cycle (C T ) of each dilution sample during 40 cycles of real-time PCR (95°C denaturation for 15 sec, 65°C anneal/extension for 60 sec).

Perforin (PRF1) Human ELISA Kit - ab46068 from Abcam

Category ELISA Kit
Prices $489.00, $734.00
Sizes 1 x 96 tests, 2 x 96 tests
Sensitivity < 40 pg/ml
Range 62.5 pg/ml - 2000 pg/ml
Perforin measured in cell culture supernatants (duplicates +/- SD).

Perforin Human ELISA Set (without plates) - ab83709 from Abcam

Prices $537.00, $620.00
Sizes 5 x 96 tests, 10 x 96 tests
Sensitivity < 40 pg/ml
Range 62.5 pg/ml - 2000 pg/ml
Perforin Standard curve ranging from 62.5 to 2000 pg/mL

Perforin Human Elispot Kit - ab46620 from Abcam

Category ELISA Kit
Prices $937.00, $1,563.00
Sizes 5 x 96 tests, 10 x 96 tests

Perforin Human Elispot Kit - ab62943 from Abcam

Category ELISA Kit
Prices $339.00, $937.00
Sizes 1 x 96 tests, 5 x 96 tests

Perforin Human Elispot Kit (Reagents for 10 x 96 tests, without plates) - ab46635 from Abcam

Category ELISA Kit
Prices $1,021.00
Sizes 10 x 96 tests

Perforin Human Elispot Kit (without plates) - ab46582 from Abcam

Category ELISA Kit
Prices $729.00, $1,021.00, $1,359.00, $1,614.00
Sizes 5 x 96 tests, 10 x 96 tests, 15 x 96 tests, 20 x 96 tests

Mouse PRF1 / Perforin ELISA Kit (Sandwich ELISA) - LS-F6493 from LifeSpan Bioscience

Category ELISA Kit
Prices $700.00
Sizes 1 plate
Sensitivity 0.156 ng/ml
Range 1.56-100 ng/ml

Human PRF1 / Perforin ELISA Kit (Sandwich ELISA) - LS-F4557 from LifeSpan Bioscience

Category ELISA Kit
Prices $690.00
Sizes 1 plate
Sensitivity 0.62 ng/ml
Range 1.56-100 ng/ml

Rat PRF1 / Perforin ELISA Kit (Sandwich ELISA) - LS-F9922 from LifeSpan Bioscience

Category ELISA Kit
Prices $740.00
Sizes 1 plate
Sensitivity 0.059 ng/ml
Range 1.56-100 ng/ml

Human PRF1 / Perforin ELISA Kit (Sandwich ELISA) - LS-F12308 from LifeSpan Bioscience

Category ELISA Kit
Prices $600.00
Sizes 1 plate
Sensitivity 15.6
Range 1.56-100 ng/ml

Human Perforin 1 ELISA kit - 55R-2059 from Fitzgerald

Category ELISA Kit
Prices $505.00
Sizes 96 tests

Perforin 1 (PRF1), Human, ELISA Kit - SEB317Hu from ARP American Research Products

Category ELISA Kit
Prices $685.00
Sizes 96 t
Sensitivity 0.56ng/mL
Range 1.56-100ng/mL

Perforin-1 (PRF1/PFP), Human, ELISA Kit - CSB-E09313h from ARP American Research Products

Category ELISA Kit
Prices $338.00, $468.00, $469.00, $471.00
Sizes 48 t, 96 t, 5 x 96t, 10 x 96t
Sensitivity 0.078 ng/ml
Range 0.312 ng/ml-20 ng/ml

Perforin/Pore-Forming Protein, PF/PFP, Human, ELISA Kit - YHB2271Hu from ARP American Research Products

Category ELISA Kit

PRF1 (Human) IP-WB Antibody Pair - H00005551-PW1 from Acris Antibodies

Category Antibody Pair
Prices $610.00
Sizes 1 set

PRF1 (Human) IP-WB Antibody Pair - H00005551-PW2 from Acris Antibodies

Category Antibody Pair
Prices $610.00
Sizes 1 set

Perforin, ELISA Kit - MBS268289 from MyBioSource

Category ELISA Kit
Prices $315.00, $490.00, $1,825.00, $3,425.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Perforin, ELISA Kit - MBS038151 from MyBioSource

Category ELISA Kit
Prices $435.00, $600.00, $2,650.00, $5,205.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Perforin, ELISA Kit - MBS051727 from MyBioSource

Category ELISA Kit
Prices $435.00, $600.00, $2,650.00, $5,205.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Perforin, ELISA Kit - MBS084762 from MyBioSource

Category ELISA Kit
Prices $435.00, $600.00, $2,650.00, $5,205.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Perforin, ELISA Kit - MBS045850 from MyBioSource

Category ELISA Kit
Prices $435.00, $600.00, $2,650.00, $5,205.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Perforin, ELISA Kit - MBS019484 from MyBioSource

Category ELISA Kit
Prices $435.00, $600.00, $2,650.00, $5,205.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Perforin, ELISA Kit - MBS037661 from MyBioSource

Category ELISA Kit
Prices $435.00, $600.00, $2,650.00, $5,205.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells

Perforin, ELISA Kit - MBS269605 from MyBioSource

Category ELISA Kit
Prices $315.00, $490.00, $1,825.00, $3,425.00
Sizes 48 -strip-wells, 96 -strip-wells, 5x96-strip-wells, 10x96-strip-wells
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