| Gene Symbol | PRF1 |
|---|---|
| Entrez Gene | 5551 |
| Alt Symbol | FLH2, HPLH2, P1, PFN1, PFP |
| Species | Human |
| Gene Type | protein-coding |
| Description | perforin 1 (pore forming protein) |
| Other Description | cytolysin|lymphocyte pore forming protein|lymphocyte pore-forming protein|perforin-1 |
| Swissprots | Q59F57 Q86WX7 P14222 B2R6X4 |
| Accessions | AAA60167 AGS13731 AGS13732 AHC72295 AHC72296 EAW54407 P14222 AB209604 BAD92841 AK312754 BAG35621 BC047695 AAH47695 BC063043 AAH63043 BQ051559 BQ654088 DA816891 DA941244 FJ555237 ACU25794 L40557 AAA63618 M28393 AAA60065 X13224 CAA31612 NM_001083116 NP_001076585 NM_005041 NP_005032 |
| Function | Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. {ECO:0000269|PubMed:20038786, ECO:0000269|PubMed:20225066, ECO:0000269|PubMed:20889983, ECO:0000269|PubMed:21037563, ECO:0000269|PubMed:9058810, ECO:0000269|PubMed:9164947}. |
| Subcellular Location | Cytoplasmic granule lumen. Secreted. Cell membrane; Multi-pass membrane protein. Endosome lumen. Note=Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes. |
| Top Pathways | Natural killer cell mediated cytotoxicity, Autoimmune thyroid disease, Allograft rejection, Graft-versus-host disease, Type I diabetes mellitus |
Perforin 1 CRISPR/Cas9 KO Plasmid (h) - sc-400976 from Santa Cruz Biotechnology
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Perforin 1 HDR Plasmid (h) - sc-400976-HDR from Santa Cruz Biotechnology
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Perforin 1 Double Nickase Plasmid (h) - sc-400976-NIC from Santa Cruz Biotechnology
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Perforin 1 Double Nickase Plasmid (h2) - sc-400976-NIC-2 from Santa Cruz Biotechnology
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Perforin 1 CRISPR Activation Plasmid (h) - sc-400976-ACT from Santa Cruz Biotechnology
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Perforin 1 CRISPR Activation Plasmid (h2) - sc-400976-ACT-2 from Santa Cruz Biotechnology
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Perforin 1 Lentiviral Activation Particles (h) - sc-400976-LAC from Santa Cruz Biotechnology
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Perforin 1 Lentiviral Activation Particles (h2) - sc-400976-LAC-2 from Santa Cruz Biotechnology
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Perforin 1 siRNA (h) - sc-42592 from Santa Cruz Biotechnology
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Perforin 1 shRNA Plasmid (h) - sc-42592-SH from Santa Cruz Biotechnology
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Perforin 1 shRNA (h) Lentiviral Particles - sc-42592-V from Santa Cruz Biotechnology
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Perforin 1 siRNA (r) - sc-270073 from Santa Cruz Biotechnology
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Perforin 1 shRNA Plasmid (r) - sc-270073-SH from Santa Cruz Biotechnology
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Perforin 1 shRNA (r) Lentiviral Particles - sc-270073-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU138981 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN17394 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND17394 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR06201 from Sigma-Aldrich
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PRF1 siRNA (Human) - CRH3727 from Cohesion Biosciences
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shRNA set against Human PRF1 (NM_005041.4) - SHH382468 from Creative biogene
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shRNA set against Human PRF1(NM_005041.4) - SHR138774 from Creative biogene
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shRNA set against Human PRF1(NM_001083116.1) - SHR138810 from Creative biogene
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Human PRF1 siRNA - orb264229 from Biorbyt
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PRF1 shRNA Plasmid (Human) - CHH3727 from Cohesion Biosciences
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PRF1 miRNA 3'UTR clone - MiUTR1H-08246 from Creative biogene
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