SHPRH

Gene Symbol SHPRH
Entrez Gene 257218
Alt Symbol bA545I5.2
Species Human
Gene Type protein-coding
Description SNF2 histone linker PHD RING helicase, E3 ubiquitin protein ligase
Other Description 2610103K11Rik|E3 ubiquitin-protein ligase SHPRH|SNF2, histone-linker, PHD and RING finger domain-containing helicase
Swissprots Q5VV79 Q149N8 Q68DS5 Q7Z5J5 Q8N1S8 Q8NBR7 Q149N9 Q8IVE8 Q8IWQ9
Accessions EAW47836 EAW47837 EAW47838 EAW47839 Q149N8 AA315737 AB095943 BAC23119 AK075318 BAC11544 AK094944 BAC04459 AK126959 BAG54410 AK130768 AL833521 AY161136 AAO26201 AY163808 AAO06907 BC113089 AAI13090 BC117685 AAI17686 BC117686 AAI17687 BX648322 CR749290 CAH18145 XM_006715439 XP_006715502 XM_006715440 XP_006715503 XM_006715441 XP_006715504 XM_006715443 XP_006715506 XM_011535719 XP_011534021 XM_011535720 XP_011534022 XM_011535721 XP_011534023 XM_011535722 XP_011534024 XR_245512 XR_942390 XR_942391 XR_942392 XR_942393 XR_942395 NM_001042683 NP_001036148 NM_173082 NP_775105
Function E3 ubiquitin-protein ligase involved in DNA repair. Upon genotoxic stress, accepts ubiquitin from the UBE2N-UBE2V2 E2 complex and transfers it to 'Lys-164' of PCNA which had been monoubiquitinated by UBE2A/B-RAD18, promoting the formation of non-canonical poly-ubiquitin chains linked through 'Lys-63'. {ECO:0000269|PubMed:17108083, ECO:0000269|PubMed:17130289, ECO:0000269|PubMed:18719106}.
Tissue Specificity Broadly expressed. {ECO:0000269|PubMed:12837266}.

SHPRH (E-10) - sc-514395 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SHPRH (E-10): sc-514395. Western blot analysis of SHPRH expression in HeLa (A), SH-SY5Y (B), T98G (C), Jurkat (D) and K-562 (E) whole cell lysates.

SHPRH (H-205) - sc-368548 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

SHPRH (Y-20) - sc-69324 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SHPRH (Y-20): sc-69324. Western blot analysis of SHPRH expression in HeLa whole cell lysate.

Anti-SHPRH antibody - ab80129 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-SHPRH antibody (ab80129) at 1/1000 dilution + HEK293 whole cell lysate at 50 µgSecondaryHRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-SHPRH antibody - ab93101 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-SHPRH antibody [1H3] - ab117928 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1H3
All lanes : Anti-SHPRH antibody [1H3] (ab117928) at 1/2000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control Lane 2 : HEK293T cells transfected with pCMV6-ENTRY SHPRH cDNA Lysates/proteins at 5 µg per lane.

Anti-SHPRH antibody [2994C1a] - ab84856 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2994C1a
Anti-SHPRH antibody [2994C1a] (ab84856) + immunogen (recombinant fragment of Human SHPRH)

Anti-SHPRH Antibody (clone 7B2) - LS-C337140 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 7B2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHPRH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHPRH.

Anti-SHPRH Antibody (clone 2994C1a) - LS-C342764 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2994C1a
Left : Western blot analysis of immunized recombinant protein, using anti-SHPRH monoclonal antibody. Right : CBB staining of immunized recombinant protein.

Anti-SHPRH Antibody (clone 5G8) - LS-C337141 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5G8
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHPRH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHPRH.

Anti-SHPRH Antibody (aa515-535) IHC-plus™ - LS-B308 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-SHPRH antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B308 concentration 5 ug/ml.

Anti-SHPRH Antibody - LS-C126274 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal

Anti-SHPRH Antibody (aa521-540) - LS-C91381 from LifeSpan Bioscience

Prices $495.00
Sizes 1 mg
Host Rabbit
Clonality Polyclonal

Anti-SHPRH Antibody (clone 2994C1a) - LS-C184733 from LifeSpan Bioscience

Prices $335.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 2994C1a

Anti-SHPRH Antibody (aa251-300) - LS-C110174 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SHPRH antibody LS-C110174 Western blot of HeLa lysate.

Anti-SHPRH Antibody (clone 5A8) - LS-C172349 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5A8
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHPRH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHPRH.

Anti-SHPRH Antibody (clone 4F3) - LS-C172348 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4F3
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHPRH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHPRH.

Anti-SHPRH Antibody (clone 5D4) - LS-C172350 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5D4
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHPRH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHPRH.

Anti-SHPRH Antibody (clone 4A3) - LS-C172351 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4A3
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHPRH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHPRH.

Anti-SHPRH Antibody (clone 3F8) - LS-C172319 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F8
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHPRH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHPRH.

Anti-SHPRH Antibody (clone 6D9) - LS-C172353 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6D9
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHPRH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHPRH.

Anti-SHPRH Antibody (clone 1D12) - LS-C172354 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1D12
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHPRH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHPRH.

Anti-SHPRH Antibody (clone 1H3) - LS-C337139 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1H3
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHPRH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHPRH.

SHPRH Antibody - NBP1-57190 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: SHPRH Antibody [NBP1-57190] - Hela cell lysate, concentration 0.2-1 ug/ml.

SHPRH Antibody - NBP1-92387 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: SHPRH Antibody [NBP1-92387] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli & cytoskeleton (microtubules).
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