MASTL

Gene Symbol MASTL
Entrez Gene 84930
Alt Symbol GREATWALL, GW, GWL, MAST-L, THC2, hGWL
Species Human
Gene Type protein-coding
Description microtubule associated serine/threonine kinase-like
Other Description greatwall kinase homolog|greatwall protein kinase|microtubule-associated serine/threonine-protein kinase-like|serine/threonine-protein kinase greatwall
Swissprots Q5T8D7 Q8NCD6 Q5T8D5 Q96SJ5 Q96GX5
Accessions EAW86064 EAW86065 EAW86066 EAW86067 Q96GX5 AK027719 BAB55321 AK074804 BAC11218 AK123004 AK225319 AW408392 AW675108 BC009107 AAH09107 BC013704 BU430288 DA524606 DB150802 XM_005252630 XP_005252687 XM_005252631 XP_005252688 XM_005252632 XP_005252689 XM_006717518 XP_006717581 XM_006717519 XP_006717582 XM_006717520 XP_006717583 XM_011519751 XP_011518053 XR_930523 NM_001172303 NP_001165774 NM_001172304 NP_001165775 NM_032844 NP_116233
Function Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation. {ECO:0000269|PubMed:12890928, ECO:0000269|PubMed:19680222, ECO:0000269|PubMed:19793917, ECO:0000269|PubMed:20538976, ECO:0000269|PubMed:20818157}.
Subcellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Cleavage furrow. Note=During interphase is mainly nuclear, upon nuclear envelope breakdown localizes at the cytoplasm and during mitosis at the centrosomes. Upon mitotic exit moves to the cleavage furrow.

MASTL (D3J4Y) Rabbit mAb - 12069 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D3J4Y
Western blot analysis of extracts from various cell lines using MASTL (D3J4Y) Rabbit mAb.

MASTL (C-16) - sc-243417 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

MASTL (N-16) - sc-243415 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-MASTL antibody - ab86387 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma (left) and lung carcinoma (right) tissues labelling MASTL with ab86387 at 1/1000 (1µg/ml) and 1/500 (2µg/ml). Detection: DAB and a DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100).

Anti-MASTL antibody - ab135637 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ab135637 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab135637 at 1/1000 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MASTL antibody - ab70118 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-MASTL antibody (ab70118) at 1/500 dilutionLane 1 : HepG2 cell extractLane 2 : HepG2 cell extract with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-MASTL antibody - ab113268 from Abcam

Prices $428.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab113268, at a 1/250 dilution, staining MASTL in formalin fixed, paraffin embedded Human ovarian carcinoma by Immunohistochemistry. Detection: DAB staining.

Anti-MASTL antibody [RIPLY 74C] - ab166647 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone RIPLY 74C
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MASTL with ab166647 at 1/15 dilution.

Anti-MASTL antibody - C-terminal - ab199925 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-MASTL antibody - C-terminal (ab199925) at 1/1000 dilutionLane 1 : Total cell extracts from mouse thymusLane 2 : Total cell extracts from HeLa cellsLane 3 : Total cell extracts from Jurkat cells

Anti-MASTL / GW Antibody (clone 235CT7.8.2.3, APC) - LS-C212496 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 235CT7.8.2.3

Anti-MASTL / GW Antibody (clone 235CT7.8.2.3, Biotin) - LS-C212497 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 235CT7.8.2.3

Anti-MASTL / GW Antibody (clone 235CT7.8.2.3, Azide-free, HRP) - LS-C212499 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 235CT7.8.2.3

Anti-MASTL / GW Antibody (clone 235CT7.8.2.3, FITC) - LS-C212498 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 235CT7.8.2.3

Anti-MASTL / GW Antibody (clone 235CT7.8.2.3, PE) - LS-C212500 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 235CT7.8.2.3

Anti-MASTL / GW Antibody (aa561-589, AP) - LS-C215741 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MASTL / GW Antibody (aa561-589, APC) - LS-C215743 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MASTL / GW Antibody (aa561-589, Biotin) - LS-C215744 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MASTL / GW Antibody (aa561-589, FITC) - LS-C215745 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MASTL / GW Antibody (aa561-589, Azide-free, HRP) - LS-C215746 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MASTL / GW Antibody (aa561-589, PE) - LS-C215747 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MASTL / GW Antibody - LS-C164045 from LifeSpan Bioscience

Prices $295.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
MASTL western blot of HepG2 cell line lysates (35 ug/lane). The MASTL antibody detected the MASTL protein (arrow).

Anti-MASTL / GW Antibody (AP) - LS-C220011 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MASTL / GW Antibody (Biotin) - LS-C220013 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-MASTL / GW Antibody (aa561-589) - LS-C164481 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Mouse Mastl Antibody western blot of mouse liver tissue lysates (35 ug/lane). The Mastl antibody detected the Mastl protein (arrow).

Anti-MASTL / GW Antibody (APC) - LS-C220012 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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