MASTL
| Gene Symbol | MASTL |
|---|---|
| Entrez Gene | 84930 |
| Alt Symbol | GREATWALL, GW, GWL, MAST-L, THC2, hGWL |
| Species | Human |
| Gene Type | protein-coding |
| Description | microtubule associated serine/threonine kinase-like |
| Other Description | greatwall kinase homolog|greatwall protein kinase|microtubule-associated serine/threonine-protein kinase-like|serine/threonine-protein kinase greatwall |
| Swissprots | Q5T8D7 Q8NCD6 Q5T8D5 Q96SJ5 Q96GX5 |
| Accessions | EAW86064 EAW86065 EAW86066 EAW86067 Q96GX5 AK027719 BAB55321 AK074804 BAC11218 AK123004 AK225319 AW408392 AW675108 BC009107 AAH09107 BC013704 BU430288 DA524606 DB150802 XM_005252630 XP_005252687 XM_005252631 XP_005252688 XM_005252632 XP_005252689 XM_006717518 XP_006717581 XM_006717519 XP_006717582 XM_006717520 XP_006717583 XM_011519751 XP_011518053 XR_930523 NM_001172303 NP_001165774 NM_001172304 NP_001165775 NM_032844 NP_116233 |
| Function | Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation. {ECO:0000269|PubMed:12890928, ECO:0000269|PubMed:19680222, ECO:0000269|PubMed:19793917, ECO:0000269|PubMed:20538976, ECO:0000269|PubMed:20818157}. |
| Subcellular Location | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Cleavage furrow. Note=During interphase is mainly nuclear, upon nuclear envelope breakdown localizes at the cytoplasm and during mitosis at the centrosomes. Upon mitotic exit moves to the cleavage furrow. |
MASTL CRISPR/Cas9 KO Plasmid (h) - sc-408989 from Santa Cruz Biotechnology
|
||||||||
MASTL HDR Plasmid (h) - sc-408989-HDR from Santa Cruz Biotechnology
|
||||||||
MASTL Double Nickase Plasmid (h) - sc-408989-NIC from Santa Cruz Biotechnology
|
||||||||
MASTL Double Nickase Plasmid (h2) - sc-408989-NIC-2 from Santa Cruz Biotechnology
|
||||||||
MASTL CRISPR Activation Plasmid (h) - sc-408989-ACT from Santa Cruz Biotechnology
|
||||||||
MASTL CRISPR Activation Plasmid (h2) - sc-408989-ACT-2 from Santa Cruz Biotechnology
|
||||||||
MASTL Lentiviral Activation Particles (h) - sc-408989-LAC from Santa Cruz Biotechnology
|
||||||||
MASTL Lentiviral Activation Particles (h2) - sc-408989-LAC-2 from Santa Cruz Biotechnology
|
||||||||
MASTL siRNA (h) - sc-90720 from Santa Cruz Biotechnology
|
||||||||
MASTL shRNA Plasmid (h) - sc-90720-SH from Santa Cruz Biotechnology
|
||||||||
MASTL shRNA (h) Lentiviral Particles - sc-90720-V from Santa Cruz Biotechnology
|
||||||||
MASTL RNAi - H00084930-R02 from Novus Biologicals
|
||||||||
MISSION® esiRNA - EHU076481 from Sigma-Aldrich
|
||||||||
MISSION® siRNA - SIHK1272 from Sigma-Aldrich
|
||||||||
MISSION® siRNA - SIHK1274 from Sigma-Aldrich
|
||||||||
MISSION® siRNA - SIHK1273 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit - CKOZFN13057 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND13057 from Sigma-Aldrich
|
||||||||
MISSION® 3′UTR Lenti GoClone™ - HUTR03523 from Sigma-Aldrich
|
||||||||
MASTL siRNA (Human) - CRJ3702 from Cohesion Biosciences
|
||||||||
shRNA set against Human MASTL (NM_032844.3) - SHH338551 from Creative biogene
|
||||||||
Human MASTL siRNA - orb274203 from Biorbyt
|
||||||||
MASTL shRNA Plasmid (Human) - CHJ3702 from Cohesion Biosciences
|
||||||||
MASTL miRNA 3'UTR clone - MiUTR3H-09853 from Creative biogene
|