DNAJC27

Gene Symbol DNAJC27
Entrez Gene 51277
Alt Symbol RBJ, RabJS
Species Human
Gene Type protein-coding
Description DnaJ (Hsp40) homolog, subfamily C, member 27
Other Description Ras-associated protein Rap1|dnaJ homolog subfamily C member 27|rab and DnaJ domain-containing protein
Swissprots Q5JV88 Q86Y24 Q9NZQ0
Accessions AAX93052 AAY14788 EAX00735 EAX00736 EAX00737 EAX00738 Q9NZQ0 AF178983 AAF44347 AK294726 BAG57873 AL117490 AL137731 CAI46214 AY094594 AAM22521 BC034049 AAH34049 BK001284 DAA01323 XM_011532902 XP_011531204 XR_244938 NM_001198559 NP_001185488 NM_016544 NP_057628
Function GTPase which can activate the MEK/ERK pathway and induce cell transformation when overexpressed. May act as a nuclear scaffold for MAPK1, probably by association with MAPK1 nuclear export signal leading to enhanced ERK1/ERK2 signaling. {ECO:0000250|UniProtKB:Q8CFP6}.
Subcellular Location Nucleus {ECO:0000250|UniProtKB:Q8CFP6}.
Tissue Specificity Overexpressed in gastrointestinal cancers; expression correlates with later tumor-node-metastasis stages of colorectal cancers. {ECO:0000269|PubMed:24746703}.

RBJ (E-2) - sc-390736 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
RBJ (E-2): sc-390736. Western blot analysis of RBJ expression in non-transfected (A) and human RBJ transfected (B) CMV whole cell lysates.

RBJ (FL-273) - sc-135327 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
RBJ (FL-273): sc-135327. Western blot analysis of RBJ expression in non-transfected (A) and human RBJ transfected (B) CMV whole cell lysates.

Anti-RBJ antibody - ab112932 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-RBJ antibody (ab112932) at 1/100 dilution + Human placenta tissue lysates at 35 µg

Anti-RBJ antibody - ab67702 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-RBJ antibody (ab67702) at 1/500 dilutionLane 1 : RBJ transfected 293T cell lysateLane 2 : non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-RBJ antibody - ab98024 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-RBJ antibody (ab98024) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human Fetal Kidney Lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution

Anti-RBJ antibody - ab128336 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-RBJ antibody (ab128336) at 1 µg/ml + Mouse kidney lysate at 10 µgdeveloped using the ECL technique

Anti-DNAJC27 Antibody (aa1-30, AP) - LS-C234698 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DNAJC27 Antibody (aa1-30, APC) - LS-C234701 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DNAJC27 Antibody (aa1-30, Biotin) - LS-C234700 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DNAJC27 Antibody (aa1-30, FITC) - LS-C234702 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DNAJC27 Antibody (aa1-30) - LS-C320899 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DNAJC27 Antibody (aa1-30, Azide-free, HRP) - LS-C249855 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DNAJC27 Antibody (aa1-30, PE) - LS-C234703 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DNAJC27 Antibody (aa52-101) - LS-C151333 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
DNAJC27 antibody Western Blot of Mouse Kidney.

Anti-DNAJC27 Antibody (aa143-192) - LS-C107663 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
DNAJC27 antibody LS-C107663 Western blot of Fetal Kidney lysate.

Anti-DNAJC27 Antibody (aa1-30) - LS-C167477 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
DNAJC27 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human kidney tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

RBJ Antibody - NBP2-38333 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: RBJ Antibody [NBP2-38333] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11<br/>Lane 2: RT-4<br/>Lane 3: U-251 MG<br/>Lane 4: Human Plasma<br/>Lane 5: Liver<br/>Lane 6: Tonsil

RBJ Antibody - NBP1-80857 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: RBJ Antibody [NBP1-80857] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

RBJ Antibody - NBP1-58940 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: RBJ Antibody [NBP1-58940] - Human kidney lysate, concentration 0.2-1 ug/ml.

RBJ Antibody - H00051277-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: RBJ Antibody [H00051277-B01] - analysis of RBJ expression in transfected 293T cell line by RBJ MaxPab polyclonal antibody (B01). Lane 1: RBJ transfected lysate (30.90 KDa). Lane 2: Non-transfected lysate.

RBJ Antibody - H00051277-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: RBJ Antibody [H00051277-B01P] - Analysis of DNAJC27 expression in transfected 293T cell line by DNAJC27 polyclonal antibody. Lane 1: DNAJC27 transfected lysate(30.03 KDa). Lane 2: Non-transfected lysate.

RBJ Antibody (3C1) - H00051277-M09 from Novus Biologicals

Prices $359.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3C1

Anti-DNAJC27 antibody produced in rabbit - HPA036816 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-DNAJC27: <B>Cat. No. HPA036816</B>: Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear membrane positivity in tubular cells.

Monoclonal Anti-RBJ antibody produced in mouse - SAB1403241 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3C1
<B>Immunoblotting</B><BR/>Monoclonal Anti-RBJ: <B>Cat. No. SAB1403241</B>: Antibody concentration: 1 μg/mL. Specific band of ~56.1 kDa using immunogen protein lysate.

Anti-DNAJC27 (2-13) antibody produced in rabbit - SAB1105862 from Sigma-Aldrich

Prices $279.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human DNAJC27 was separated on SDS-PAGE, blotted with Anti-DNAJC27 (2-13) (<B>Cat. No. SAB1105862</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500<BR/>2. Antibody dilution 1:500 + DNAJC27 immunizing peptide (human, 2-13)
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