ATG4A

Gene Symbol	ATG4A
Entrez Gene	115201
Alt Symbol	APG4A, AUTL2
Species	Human
Gene Type	protein-coding
Description	autophagy related 4A, cysteine peptidase
Other Description	APG4 autophagy 4 homolog A\|ATG4 autophagy related 4 homolog A\|AUT-like 2 cysteine endopeptidase\|AUT-like 2, cysteine endopeptidase\|autophagin 2\|autophagin-2\|autophagy-related cysteine endopeptidase 2\|autophagy-related protein 4 homolog A\|cysteine protease ATG4A\|hAPG4A
Swissprots	O95534 Q8WYN0 D3DUY0 A6NCH2 Q5JYY9 B2RAZ7 Q5JYZ0 Q96KQ1 Q86VE5 Q96KQ0
Accessions	EAX02689 EAX02690 EAX02691 EAX02692 EAX02693 EAX02694 EAX02695 EAX02696 Q8WYN0 AA648264 AB066214 BAB83889 AJ320508 CAC69076 AJ320509 CAC69077 AJ504651 CAD43218 AK054927 AK123287 AK293533 BAG57015 AK314429 BAG37044 BC041862 AAH41862 BC061696 AAH61696 BM541631 HQ447703 ADQ32188 XM_005262064 XP_005262121 XM_011530840 XP_011529142 XM_011530841 XP_011529143 XM_011530842 XP_011529144 XM_011530843 XP_011529145 XM_011530844 XP_011529146 XM_011530845 XP_011529147 NM_052936 NP_443168 NM_178270 NP_840054 NM_178271
Function	Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms. {ECO:0000269\|PubMed:12473658, ECO:0000269\|PubMed:15169837, ECO:0000269\|PubMed:17347651, ECO:0000269\|PubMed:21177865, ECO:0000269\|PubMed:21245471, ECO:0000269\|PubMed:22302004}.
Subcellular Location	Cytoplasm {ECO:0000250}.
Tissue Specificity	Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver. {ECO:0000269\|PubMed:12446702, ECO:0000269\|PubMed:15169837}.
Top Pathways	Regulation of autophagy

Atg4A (D62C10) Rabbit mAb - 7613 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	D62C10

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-) or SignalSilence ® Atg4A siRNA I #6427 (+), using Atg4A (D62C10) Rabbit mAb (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The Atg4A (D62C10) Rabbit mAb confirms silencing of Atg4A expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

Atg4a (D-14) - sc-131432 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Atg4a (D-14): sc-131432. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

Atg4a (E-12) - sc-131433 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Atg4a (Q-12) - sc-131435 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-ATG4A antibody - ab187508 from Abcam

Prices	$370.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat brain tissue labelling ATG4A with ab187508 at 1/200. Magnification: 400x.

Anti-ATG4A antibody - ab64743 from Abcam

Host	Sheep
Clonality	Polyclonal

Anti-ATG4A antibody [EPR4122] - ab108322 from Abcam

Prices	$357.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR4122

All lanes : Anti-ATG4A antibody [EPR4122] (ab108322) at 1/1000 dilutionLane 1 : Fetal liver lysateLane 2 : HepG2 lysateLane 3 : Jurkat lysateLane 4 : Fetal brain lysateLysates/proteins at 10 µg per lane.SecondaryStandard HRP labelled goat anti-rabbit at 1/2000 dilution

Anti-ATG4A antibody - N-terminal - ab173791 from Abcam

Prices	$370.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-ATG4A antibody - N-terminal (ab173791) at 1/100 dilutionLane 1 : Mouse kidney tissue lysateLane 2 : Mouse heart tissue lysateLysates/proteins at 12.5 µg per lane.

Anti-ATG4A Antibody (aa2-398) - LS-C34999 from LifeSpan Bioscience

Prices	$605.00
Sizes	100 µg
Host	Sheep
Clonality	Polyclonal

Anti-ATG4A Antibody (aa2-398) - LS-C125090 from LifeSpan Bioscience

Prices	$465.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Anti-ATG4A Antibody (aa100-114) - LS-C188038 from LifeSpan Bioscience

Prices	$395.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ATG4A Antibody (aa81-130) - LS-C119886 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

IHC of paraffin-embedded human breast carcinoma tissue, using ATG4A Antibody. The picture on the right is treated with the synthesized peptide.

Anti-ATG4A Antibody (aa251-300) - LS-C116218 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

ATG4A antibody LS-C116218 Western blot of Fetal Spleen lysate.

Anti-ATG4A Antibody - LS-C341755 from LifeSpan Bioscience

Prices	$385.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-ATG4A Antibody (aa91-140) - LS-C201726 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of Human A549. ATG4A antibody dilution 1.0 ug/ml.

Anti-ATG4A Antibody (aa7-37) - LS-C156583 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Anti-ATG4A Antibody (C-Terminus) IHC-plus™ - LS-B12105 from LifeSpan Bioscience

Prices	$395.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

Anti-ATG4A Antibody - LS-C332196 from LifeSpan Bioscience

Prices	$255.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry of paraffin-embedded rat brain using ATG4A antibody at dilution of 1:200 (400x lens).

Anti-ATG4A Antibody (aa369-398) - LS-C156585 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with Autophagy APG4A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-ATG4A Antibody (Internal, clone EPR4122) IHC-plus™ - LS-B6242 from LifeSpan Bioscience

Prices	$395.00
Sizes	50 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR4122

Anti-ATG4A antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6242 dilution 1:50.

ATG4A Antibody (514914) - MAB4324 from Novus Biologicals

Prices	$99.00, $249.00
Sizes	25 µg, 100 µg
Host	Mouse
Clonality	Monoclonal
Clone	514914

ATG4A Antibody - NBP1-89044 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: ATG4A Antibody [NBP1-89044] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-50

ATG4A Antibody [Unconjugated] - AF4324 from Novus Biologicals

Prices	$99.00, $359.00
Sizes	25 µg, 100 µg
Host	Sheep
Clonality	Polyclonal

Western blot shows lysates of HUVEC human umbilical vein endothelial cells, MCF‑7 human breast cancer cell line, and human skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human ATG4A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4324) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ATG4A at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

ATG4A Antibody - NB110-74831 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Sheep
Clonality	Polyclonal

ATG4A Polyclonal Antibody - bs-3873R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

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