BUB3

Gene Symbol BUB3
Entrez Gene 9184
Alt Symbol BUB3L, hBUB3
Species Human
Gene Type protein-coding
Description BUB3 mitotic checkpoint protein
Other Description BUB3 budding uninhibited by benzimidazoles 3 homolog|budding uninhibited by benomyl|budding uninhibited by benzimidazoles 3 homolog|mitotic checkpoint component|mitotic checkpoint protein BUB3
Swissprots O43684 O43685 B2R6E7 A6NJ42 D3DRE9
Accessions AAL17976 EAW49284 EAW49285 EAW49286 EAW49287 O43684 AF047472 AAC28438 AF047473 AAC28439 AF053304 AAC06258 AF081496 AAC36307 AK226060 AK293255 BAG56787 AK307958 AK312546 BAG35444 BC005138 AAH05138 BC022438 AAH22438 BM989334 BP328027 BU932619 BX647392 DQ891852 ABM82778 DQ895042 ABM85968 NM_001007793 NP_001007794 NM_004725 NP_004716
Function Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. {ECO:0000269|PubMed:10198256, ECO:0000269|PubMed:15525512, ECO:0000269|PubMed:18199686}.
Subcellular Location Nucleus. Chromosome, centromere, kinetochore {ECO:0000250}. Note=Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition. {ECO:0000250}.
Top Pathways Cell cycle, HTLV-I infection

Bub3 (D8G6) Rabbit mAb - 8194 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D8G6
Western blot analysis of extracts from various cell lines using Bub3 (D8G6) Rabbit mAb.

Bub3 (D4F9) Rabbit mAb - 8195 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4F9
Western blot analysis of extracts from various cell lines using Bub3 (D4F9) Rabbit mAb.

Bub3 Antibody - 3049 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using Bub3 Antibody.

BUB3 (E-7) - sc-376506 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
BUB3 (E-7): sc-376506. Western blot analysis of BUB3 expression in Jurkat (A) and HeLa (B) nuclear extracts.

BUB3 (C-18) - sc-19397 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

BUB3 (H-100) - sc-28258 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
BUB3 (H-100): sc-28258. Western blot analysis of BUB3 expression in Jurkat nuclear extract.

BUB3 (N-15) - sc-19395 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

BUB3 (31) - sc-136217 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
BUB3 (31): sc-136217. Western blot analysis of BUB3 expression in Jurkat (A) and NIH/3T3 (B) nuclear extracts.

Anti-Bub3 antibody - ab4180 from Abcam

Prices $392.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Bub3 antibody (ab4180) at 1/500 dilution + HeLa Nuclear Extract at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

Anti-Bub3 antibody - ab88015 from Abcam

Prices $359.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Bub3 antibody (ab88015) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysate

Anti-Bub3 antibody - ab69600 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Bub3 antibody (ab69600) at 1/500 dilutionLane 1 : Extract from HeLa cellsLane 2 : Extract from Jurkat cellsLane 3 : Extract from 293 cellsLane 4 : Extract from Jurkat cells with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.

Anti-Bub3 antibody - ab137421 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-Bub3 antibody (ab137421) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

Anti-Bub3 antibody [EPR5319(2)] - ab133699 from Abcam

Prices $385.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5319(2)
All lanes : Anti-Bub3 antibody [EPR5319(2)] (ab133699) at 1/10000 dilutionLane 1 : Jurkat cell lysateLane 2 : HeLa cell lysateLane 3 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti Rabbit at 1/2000 dilution

Anti-Bub3 antibody [EPR5319(2)] (Alexa Fluor® 488) - ab200803 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5319(2)
ab200803 staining Bub3 in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200803 at 1/200 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Bub3 antibody [EPR5319(2)] (HRP) - ab200805 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5319(2)
All lanes : Anti-Bub3 antibody [EPR5319(2)] (HRP) (ab200805) at 1/5000 dilutionLane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.developed using the ECL techniquePerformed under reducing conditions.

Anti-Bub3 antibody [EPR5320(2)] - ab131157 from Abcam

Prices $379.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5320(2)
All lanes : Anti-Bub3 antibody [EPR5320(2)] (ab131157) at 1/1000 dilutionLane 1 : Hela cell lysateLane 2 : Jurkat cell lysateLane 3 : 293T cell lysateLysates/proteins at 10 µg per lane.

Anti-BUB3 Antibody (C-Terminus) IHC-plus™ - LS-B1340 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-BUB3 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1340 concentration 5 ug/ml.

Anti-BUB3 Antibody IHC-plus™ - LS-B4858 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-BUB3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4858 concentration 10 ug/ml.

Anti-BUB3 Antibody (aa76-90) - LS-C312796 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
BUB3 antibody. IHC(P): Rat Testis Tissue.

Anti-BUB3 Antibody (aa1-328) - LS-C125210 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-BUB3 Antibody (N-Terminus) - LS-C136992 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-BUB3 Antibody (N-Terminus) - LS-C112219 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
BUB3 antibody LS-C112219 Western blot of PANC1 cell lysate.

Anti-BUB3 Antibody (aa279-328) - LS-C119398 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from HeLa cells/Jurkat cells/293 cells, using BUB3 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-BUB3 Antibody (Internal, clone EPR5320(2)) - LS-C154577 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5320(2)
Western blot analysis on (A) HeLa, (B) Jurkat, and (C) 293T cell lysates using anti-BUB3 antibody.

Anti-BUB3 Antibody (aa275-325) - LS-C286392 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human BUB3 by Immunohistochemistry. Sample: FFPE section of human prostate adenocarcinoma. Antibody: Affinity purified rabbit anti-BUB3 used at a dilution of 1:250.
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