| Gene Symbol | BUB3 |
|---|---|
| Entrez Gene | 9184 |
| Alt Symbol | BUB3L, hBUB3 |
| Species | Human |
| Gene Type | protein-coding |
| Description | BUB3 mitotic checkpoint protein |
| Other Description | BUB3 budding uninhibited by benzimidazoles 3 homolog|budding uninhibited by benomyl|budding uninhibited by benzimidazoles 3 homolog|mitotic checkpoint component|mitotic checkpoint protein BUB3 |
| Swissprots | O43684 O43685 B2R6E7 A6NJ42 D3DRE9 |
| Accessions | AAL17976 EAW49284 EAW49285 EAW49286 EAW49287 O43684 AF047472 AAC28438 AF047473 AAC28439 AF053304 AAC06258 AF081496 AAC36307 AK226060 AK293255 BAG56787 AK307958 AK312546 BAG35444 BC005138 AAH05138 BC022438 AAH22438 BM989334 BP328027 BU932619 BX647392 DQ891852 ABM82778 DQ895042 ABM85968 NM_001007793 NP_001007794 NM_004725 NP_004716 |
| Function | Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. {ECO:0000269|PubMed:10198256, ECO:0000269|PubMed:15525512, ECO:0000269|PubMed:18199686}. |
| Subcellular Location | Nucleus. Chromosome, centromere, kinetochore {ECO:0000250}. Note=Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition. {ECO:0000250}. |
| Top Pathways | Cell cycle, HTLV-I infection |
BUB3 CRISPR/Cas9 KO Plasmid (h) - sc-402439 from Santa Cruz Biotechnology
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BUB3 HDR Plasmid (h) - sc-402439-HDR from Santa Cruz Biotechnology
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BUB3 Double Nickase Plasmid (h) - sc-402439-NIC from Santa Cruz Biotechnology
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BUB3 Double Nickase Plasmid (h2) - sc-402439-NIC-2 from Santa Cruz Biotechnology
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BUB3 CRISPR Activation Plasmid (h) - sc-402439-ACT from Santa Cruz Biotechnology
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BUB3 CRISPR Activation Plasmid (h2) - sc-402439-ACT-2 from Santa Cruz Biotechnology
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BUB3 Lentiviral Activation Particles (h) - sc-402439-LAC from Santa Cruz Biotechnology
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BUB3 Lentiviral Activation Particles (h2) - sc-402439-LAC-2 from Santa Cruz Biotechnology
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BUB3 siRNA (h) - sc-37540 from Santa Cruz Biotechnology
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BUB3 shRNA Plasmid (h) - sc-37540-SH from Santa Cruz Biotechnology
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BUB3 shRNA (h) Lentiviral Particles - sc-37540-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU155761 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN4121 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND4121 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR09540 from Sigma-Aldrich
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BUB3 siRNA (Human) - CRH6078 from Cohesion Biosciences
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BUB3 - MBS8226599 from MyBioSource
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shRNA set against Human BUB3 (NM_004725.3) - SHH248458 from Creative biogene
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Human BUB3 siRNA - orb266580 from Biorbyt
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BUB3 shRNA Plasmid (Human) - CHH6078 from Cohesion Biosciences
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BUB3 miRNA 3'UTR clone - MiUTR3H-06211 from Creative biogene
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BUB3 miRNA 3'UTR clone - MiUTR3H-06212 from Creative biogene
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