CHMP4B

Gene Symbol CHMP4B
Entrez Gene 128866
Alt Symbol C20orf178, CHMP4A, CTPP3, CTRCT31, SNF7, SNF7-2, Shax1, VPS32B, Vps32-2, dJ553F4.4
Species Human
Gene Type protein-coding
Description charged multivesicular body protein 4B
Other Description SNF7 homolog associated with Alix 1|Snf7 homologue associated with Alix 1|charged multivesicular body protein 4b|chromatin modifying protein 4B|chromatin-modifying protein 4b|hSnf7-2|hVps32-2|vacuolar protein sorting-associated protein 32-2|vacuolar protein-sorting-associated protein 32-2
Swissprots Q9H444 E1P5N4 Q53ZD6
Accessions EAW76293 EAW76294 Q9H444 AB100261 BAC79375 AY329085 AAQ91194 AY726594 BC033859 AAH33859 BQ926070 BU150721 CT001913 NM_176812 NP_789782
Function Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities,
Subcellular Location Cytoplasm, cytosol. Late endosome membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
Tissue Specificity Widely expressed. Expressed at higher level in heart and skeletal muscle. Also expressed in brain, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung and peripheral blood lymphocytes. {ECO:0000269|PubMed:14678797}.
Top Pathways Endocytosis

CHMP4B (C-12) - sc-82556 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
CHMP4B (C-12): sc-82556. Western blot analysis of CHMP4B expression in NIH/3T3 whole cell lysate.

CHMP4B (H-40) - sc-134946 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CHMP4B (H-40): sc-134946. Western blot analysis of CHMP4B expression in EOC 20 (A) and MH-S (B) whole cell lysates and mouse brain tissue extract (C).

CHMP4B (N-12) - sc-82557 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
CHMP4B (N-12): sc-82557. Immunoperoxidase staining of formalin fixed, paraffin-embedded human rectum tissue showing cytoplasmic staining of glandular cells.

Anti-CHMP4B / CHMP4C antibody - ab76334 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-CHMP4B / CHMP4C antibody (ab76334) at 1/300 dilutionLane 1 : Rat reticulocyte whole cell lysate (0 hours after purification) at 50 µgLane 2 : Rat reticulocyte whole cell lysate (7 days after purification) at 50 µgLane 3 : Rat exosomes (36 hours culture) at 9 µgLane 4 : Rat exosomes (84 hours culture) at 9 µgLane 5 : Rat exosomes (156 hours culture) at 9 µgSecondaryHRP-conjugated anti-rabbit IgG at 1/10000 dilutiondeveloped using the ECL technique

Anti-CHMP4B antibody - ab105767 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab105767 staining CHMP4B (red) in HeLa cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed with 4% paraformaldehyden and after three washes in PBS, cells were permeabilized with PBS containing 0.5% Triton X-100 in blocking solution (PBS containing 5% goat pre-immune serum) for 30 minutes. ab105767 was diluted in blocking solution and then incubated for 1 hour. After washing in PBS, cells were incubated for 1 hour with the secondary antibody.

Anti-CHMP4B antibody - ab135154 from Abcam

Prices $372.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CHMP4B antibody - ab105768 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-CHMP4B antibody (ab105768) at 1 µg/ml + Jurkat Cell Lysate at 10 µg

Anti-CHMP4B Antibody - LS-C153395 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-CHMP4B Antibody (aa58-87, APC) - LS-C233798 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHMP4B Antibody (aa58-87, AP) - LS-C233799 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHMP4B Antibody (aa58-87, Biotin) - LS-C233800 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHMP4B Antibody (aa58-87, FITC) - LS-C233801 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHMP4B Antibody (aa58-87, PE) - LS-C233802 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHMP4B Antibody (aa58-87, Azide-free, HRP) - LS-C249667 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHMP4B Antibody (aa58-87) - LS-C320358 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-CHMP4B Antibody (aa143-192) - LS-C116541 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
CHMP4B antibody LS-C116541 Western blot of THP-1 cell lysate.

Anti-CHMP4B Antibody (aa71-120) - LS-C116540 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
CHMP4B antibody LS-C116540 Western blot of Jurkat lysate.

Anti-CHMP4B Antibody (aa58-87) - LS-C168740 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with CHMP4B Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

CHMP4B Antibody - NBP1-91782 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CHMP4B Antibody [NBP1-91782] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

CHMP4B Antibody - NBP1-56995 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: CHMP4B Antibody [NBP1-56995] - Jurkat cell lysate, concentration 0.2-1 ug/ml.

CHMP4B Antibody - NBP1-03388 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

CHMP4B+CHMP4C Polyclonal Antibody - bs-7744R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin embedded rat brain labeled with Anti-CHMP4B/CHMP4C Polyclonal Antibody, Unconjugated (bs-7744R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Anti-CHMP4B antibody produced in rabbit - SAB2105901 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-CHMP4B: <B>Cat. No. SAB2105901</B>: CHMP4B in Jurkat cell lysates with CHMP4B antibody at 1.0 μg/mL.

Anti-CHMP4B antibody produced in rabbit - SAB2105902 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-CHMP4B: <B>Cat. No. SAB2105902</B>: CHMP4B in THP-1 cell lysates with CHMP4B antibody at 1.0 μg/mL.

Anti-CHMP4B antibody produced in rabbit - HPA041401 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-CHMP4B: <B>Cat. No. HPA041401</B>: Immunohistochemical staining of human rectum shows strong granular cytoplasmic positivity in glandular cells.
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