AFAP1

Gene Symbol AFAP1
Entrez Gene 60312
Alt Symbol AFAP, AFAP-110, AFAP110
Species Human
Gene Type protein-coding
Description actin filament associated protein 1
Other Description 110 kDa actin filament-associated protein|actin filament-associated protein 1|actin filament-associated protein, 110 kDa
Swissprots Q59EY5 E9PDT7 B4DMU2 Q8N556 Q9HBY1 A8K442
Accessions EAW82360 Q8N556 AB209676 BAD92913 AF188700 AAG17055 AK127962 BAC87209 AK290807 BAF83496 AK297631 BAG60004 AL706541 BC015900 BC032777 AAH32777 BI561136 CA448961 CN308844 D25248 DA605903 DB450895 DQ585763 XM_006713908 XP_006713971 XM_006713909 XP_006713972 XM_011513544 XP_011511846 XM_011513545 XP_011511847 XM_011513546 XP_011511848 NM_001134647 NP_001128119 NM_198595 NP_940997 NM_021638
Function Can cross-link actin filaments into both network and bundle structures (By similarity). May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells. {ECO:0000250, ECO:0000269|PubMed:15485829}.
Subcellular Location Cytoplasm, cytoskeleton {ECO:0000269|PubMed:15485829}. Note=Localizes with stress fibers in quiescent cells, concentrated in cell motility structures such as lamellipodia, filopodia and membrane ruffles upon their induction.
Tissue Specificity Low expression in normal breast epithelial cell line MCF-10A and in tumorigenic breast cancer cell lines MCF- 7, T-47D and ZR-75-1. Highly expressed in the invasive breast cancer cell lines MDA-MB-231 and MDA-MB-435. Overexpressed in prostate carcinoma. {ECO:0000269|PubMed:17520695, ECO:0000269|PubMed:17885682}.

AFAP-110 (C-18) - sc-26272 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

AFAP-110 (D-11) - sc-374655 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
AFAP-110 (D-11): sc-374655. Western blot analysis of AFAP-110 expression in non-transfected: sc-117752 (A) and human AFAP-110 transfected: sc-114982 (B) 293T whole cell lysates.

AFAP-110 (E-15) - sc-26271 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
AFAP-110 (E-15): sc-26271. Western blot analysis of AFAP-110 expression in non-transfected: sc-117752 (A) and human AFAP-110 transfected: sc-114982 (B) 293T whole cell lysates.

AFAP-110 (H-71) - sc-98844 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
AFAP-110 (H-71): sc-98844. Western blot analysis of AFAP-110 expression in non-transfected: sc-117752 (A) and human AFAP-110 transfected: sc-114982 (B) 293T whole cell lysates.

AFAP-110 (N-18) - sc-26270 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-AFAP antibody - ab113037 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-AFAP antibody (ab113037) at 1/100 dilution + MDA-MB435 cell line lysates at 35 µg

Anti-AFAP antibody - ab72035 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-AFAP antibody (ab72035) at 1/500 dilutionLane 1 : AFAP transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-AFAP antibody - N-terminal - ab189972 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-AFAP antibody - N-terminal (ab189972) at 1 µg/mlLane 2 : Anti-AFAP antibody - N-terminal (ab189972) at 2 µg/mlLane 1 : HeLa cell lysateLane 2 : HeLa cell lysate

Anti-AFAP1 / AFAP Antibody (aa450-730) - LS-C125009 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-AFAP1 / AFAP Antibody (aa543-572, APC) - LS-C250995 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AFAP1 / AFAP Antibody (aa543-572, Biotin) - LS-C250996 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AFAP1 / AFAP Antibody (aa543-572, PE) - LS-C251000 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AFAP1 / AFAP Antibody (aa459-559) - LS-C306124 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-AFAP1 / AFAP Antibody (aa543-572, Azide-free, HRP) - LS-C250998 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AFAP1 / AFAP Antibody (aa543-572, FITC) - LS-C250997 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AFAP1 / AFAP Antibody (aa301-350) - LS-C112072 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
AFAP1 antibody LS-C112072 Western blot of Fetal Liver lysate.

Anti-AFAP1 / AFAP Antibody (aa543-572, AP) - LS-C250994 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AFAP1 / AFAP Antibody (aa550-580) - LS-C319361 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-AFAP1 / AFAP Antibody (aa543-572) - LS-C156129 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
AFAP1 Antibody western blot of MDA-MB435 cell line lysates (35 ug/lane). The AFAP1 antibody detected the AFAP1 protein (arrow).

Anti-AFAP1 / AFAP Antibody - LS-C332595 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using AFAP1 antibody.

Anti-AFAP1 / AFAP Antibody (aa100-386, clone 5G2) - LS-C336517 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5G2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1.

Anti-AFAP1 / AFAP Antibody (aa100-386, clone 2D1) - LS-C336518 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2D1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1.

Anti-AFAP1 / AFAP Antibody (aa100-386, clone 4A2) - LS-C338552 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4A2
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1.

Anti-AFAP1 / AFAP Antibody (aa100-386, clone 3C1) - LS-C336522 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3C1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1.

Anti-AFAP1 / AFAP Antibody (aa100-386, clone 2A3) - LS-C336523 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2A3
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1.
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