Gene Symbol | AFAP1 |
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Entrez Gene | 60312 |
Alt Symbol | AFAP, AFAP-110, AFAP110 |
Species | Human |
Gene Type | protein-coding |
Description | actin filament associated protein 1 |
Other Description | 110 kDa actin filament-associated protein|actin filament-associated protein 1|actin filament-associated protein, 110 kDa |
Swissprots | Q59EY5 E9PDT7 B4DMU2 Q8N556 Q9HBY1 A8K442 |
Accessions | EAW82360 Q8N556 AB209676 BAD92913 AF188700 AAG17055 AK127962 BAC87209 AK290807 BAF83496 AK297631 BAG60004 AL706541 BC015900 BC032777 AAH32777 BI561136 CA448961 CN308844 D25248 DA605903 DB450895 DQ585763 XM_006713908 XP_006713971 XM_006713909 XP_006713972 XM_011513544 XP_011511846 XM_011513545 XP_011511847 XM_011513546 XP_011511848 NM_001134647 NP_001128119 NM_198595 NP_940997 NM_021638 |
Function | Can cross-link actin filaments into both network and bundle structures (By similarity). May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells. {ECO:0000250, ECO:0000269|PubMed:15485829}. |
Subcellular Location | Cytoplasm, cytoskeleton {ECO:0000269|PubMed:15485829}. Note=Localizes with stress fibers in quiescent cells, concentrated in cell motility structures such as lamellipodia, filopodia and membrane ruffles upon their induction. |
Tissue Specificity | Low expression in normal breast epithelial cell line MCF-10A and in tumorigenic breast cancer cell lines MCF- 7, T-47D and ZR-75-1. Highly expressed in the invasive breast cancer cell lines MDA-MB-231 and MDA-MB-435. Overexpressed in prostate carcinoma. {ECO:0000269|PubMed:17520695, ECO:0000269|PubMed:17885682}. |
AFAP-110 CRISPR/Cas9 KO Plasmid (h) - sc-405669 from Santa Cruz Biotechnology
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AFAP-110 HDR Plasmid (h) - sc-405669-HDR from Santa Cruz Biotechnology
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AFAP-110 Double Nickase Plasmid (h) - sc-405669-NIC from Santa Cruz Biotechnology
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AFAP-110 Double Nickase Plasmid (h2) - sc-405669-NIC-2 from Santa Cruz Biotechnology
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AFAP-110 CRISPR Activation Plasmid (h) - sc-405669-ACT from Santa Cruz Biotechnology
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AFAP-110 CRISPR Activation Plasmid (h2) - sc-405669-ACT-2 from Santa Cruz Biotechnology
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AFAP-110 Lentiviral Activation Particles (h) - sc-405669-LAC from Santa Cruz Biotechnology
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AFAP-110 Lentiviral Activation Particles (h2) - sc-405669-LAC-2 from Santa Cruz Biotechnology
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AFAP-110 siRNA (h) - sc-40954 from Santa Cruz Biotechnology
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AFAP-110 shRNA Plasmid (h) - sc-40954-SH from Santa Cruz Biotechnology
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AFAP-110 shRNA (h) Lentiviral Particles - sc-40954-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU082151 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN2912 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND2912 from Sigma-Aldrich
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AFAP1 siRNA (Human) - CRJ1785 from Cohesion Biosciences
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AFAP1 - MBS8218922 from MyBioSource
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shRNA set against Human AFAP1 (NM_198595.2) - SHH232934 from Creative biogene
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AFAP1 shRNA Plasmid (Human) - CHJ1785 from Cohesion Biosciences
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