MB21D1

Gene Symbol MB21D1
Entrez Gene 115004
Alt Symbol C6orf150, cGAS, h-cGAS
Species Human
Gene Type protein-coding
Description Mab-21 domain containing 1
Other Description cGAMP synthase|cyclic GMP-AMP synthase|mab-21 domain-containing protein 1|protein MB21D1
Swissprots Q8N884 Q96E45 Q32NC9 Q5SWL0 L0L2J9 Q5SWL1 Q14CV6
Accessions EAW48769 Q8N884 AK097148 BAC04965 BC012928 AAH12928 BC108714 AAI08715 BC113606 AAI13607 BC113608 AAI13609 BC143694 AAI43695 DA375117 HQ258303 ADR83057 KC294566 AGB51853 XM_006715327 XP_006715390 NM_138441 NP_612450
Function Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double- stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production. {ECO:0000269|PubMed:21478870, ECO:0000269|PubMed:23258413, ECO:0000269|PubMed:23707061, ECO:0000269|PubMed:23707065, ECO:0000269|PubMed:23722159, ECO:0000269|PubMed:24116191, ECO:0000269|PubMed:24462292, ECO:0000269|PubMed:25131990}.
Subcellular Location Cytoplasm, cytosol {ECO:0000269|PubMed:23258413}.
Tissue Specificity Expressed in the monocytic cell line THP1. {ECO:0000269|PubMed:23258413}.
Top Pathways Cytosolic DNA-sensing pathway

C6orf150 (C-20) - sc-245860 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

C6orf150 (N-17) - sc-245858 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
C6orf150 (N-17): sc-245858. Western blot analysis of C6orf150 expression in Jurkat (A), MDA-MB-231 (B) and RT-4 (C) whole cell lysates and human tonsil tissue extract (D).

Anti-C6orf150 antibody - ab176177 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-C6orf150 antibody (ab176177) at 1/500 dilution + THP1 cells positive control at 50 µg

Anti-C6orf150 antibody - ab106985 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-C6orf150 antibody (ab106985) at 1/100 dilution + MDA-MB231 cell line lysates at 35 µg

Anti-C6orf150 / MB21D1 Antibody (aa266-295, AP) - LS-C247015 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-C6orf150 / MB21D1 Antibody (aa266-295, APC) - LS-C247016 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-C6orf150 / MB21D1 Antibody (aa266-295, Biotin) - LS-C247017 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-C6orf150 / MB21D1 Antibody (aa266-295, Azide-free, HRP) - LS-C249657 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-C6orf150 / MB21D1 Antibody (aa266-295, FITC) - LS-C247018 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-C6orf150 / MB21D1 Antibody (aa266-295, PE) - LS-C247019 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-C6orf150 / MB21D1 Antibody (aa273-303) - LS-C320335 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-C6orf150 / MB21D1 Antibody (aa266-295) - LS-C168697 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
CF150 Antibody western blot of MDA-MB231 cell line lysates (35 ug/lane). The CF150 antibody detected the CF150 protein (arrow).

cGAS Antibody - NBP1-86761 from Novus Biologicals

Prices $129.00, $419.00
Sizes 25 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: cGAS Antibody [NBP1-86761] - Analysis in human cell line THP-1.

cGAS Antibody - NBP1-70755 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

ANTI-CF150 (CENTER) antibody produced in rabbit - SAB1306094 from Sigma-Aldrich

Prices $326.00
Sizes 40 tst
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-CF150 (Center): <B>Cat. No. SAB1306094</B>: CF150 Antibody (Center) western blot analysis in MDA-MB231 cell line lysates (35 μg/lane).

Anti-C6ORF150 antibody produced in rabbit - SAB2100310 from Sigma-Aldrich

Prices $347.50
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-C6ORF150: <B>Cat. No.SAB2100310</B>: C6orf150 in Jurkat cell lysate with C6orf150 antibody at 1.0 μg/mL.

Anti-MB21D1 antibody produced in rabbit - HPA031700 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-C6ORF150: <B>Cat. No. HPA031700</B>: Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

C6orf150 antibody - middle region (ARP52607_P050) - ARP52607_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

C6orf150 Antibody - middle region (OAAB00523) - OAAB00523 from Aviva Systems Biology

Prices $289.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal

CF150-Antibody-Center - AP10510c from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Flow cytometric analysis of A549 cells using CF150 Antibody (Center) (green, Cat#AP10510c) compared to an isotype control of rabbit IgG(blue). AP10510c was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

CF150-Antibody-Center - AW5397 from Abgent, a WuXi AppTec company

Prices $110.00, $325.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-CF150 Antibody (Center) at 1:1000 dilution Lane 1: human cerebellum lysates Lane 2: human spleen lysates Lane 3: HT-29 whole cell lysates Lane 4: MCF-7 whole cell lysates Lane 5: THP-1 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

C6ORF150 antibody, Internal - GTX45743 from GeneTex

Prices $319.00
Sizes 50 μg
Host Rabbit
Clonality Polyclonal
Anti-C6ORF150 antibody, Internal used in Western blot. GTX45743

C6ORF150 antibody - 70R-4145 from Fitzgerald

Prices $345.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti -CF150, ID (MB21D1, C6orf150, Protein MB21D1, Mab-21 domain-containing protein 1) - 033804 from USBiological Life Science

Host Rabbit
Clonality Polyclonal
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