| Gene Symbol | MB21D1 |
|---|---|
| Entrez Gene | 115004 |
| Alt Symbol | C6orf150, cGAS, h-cGAS |
| Species | Human |
| Gene Type | protein-coding |
| Description | Mab-21 domain containing 1 |
| Other Description | cGAMP synthase|cyclic GMP-AMP synthase|mab-21 domain-containing protein 1|protein MB21D1 |
| Swissprots | Q8N884 Q96E45 Q32NC9 Q5SWL0 L0L2J9 Q5SWL1 Q14CV6 |
| Accessions | EAW48769 Q8N884 AK097148 BAC04965 BC012928 AAH12928 BC108714 AAI08715 BC113606 AAI13607 BC113608 AAI13609 BC143694 AAI43695 DA375117 HQ258303 ADR83057 KC294566 AGB51853 XM_006715327 XP_006715390 NM_138441 NP_612450 |
| Function | Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double- stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production. {ECO:0000269|PubMed:21478870, ECO:0000269|PubMed:23258413, ECO:0000269|PubMed:23707061, ECO:0000269|PubMed:23707065, ECO:0000269|PubMed:23722159, ECO:0000269|PubMed:24116191, ECO:0000269|PubMed:24462292, ECO:0000269|PubMed:25131990}. |
| Subcellular Location | Cytoplasm, cytosol {ECO:0000269|PubMed:23258413}. |
| Tissue Specificity | Expressed in the monocytic cell line THP1. {ECO:0000269|PubMed:23258413}. |
| Top Pathways | Cytosolic DNA-sensing pathway |
C6orf150 CRISPR/Cas9 KO Plasmid (h) - sc-403354 from Santa Cruz Biotechnology
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C6orf150 HDR Plasmid (h) - sc-403354-HDR from Santa Cruz Biotechnology
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C6orf150 Double Nickase Plasmid (h) - sc-403354-NIC from Santa Cruz Biotechnology
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C6orf150 Double Nickase Plasmid (h2) - sc-403354-NIC-2 from Santa Cruz Biotechnology
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C6orf150 CRISPR Activation Plasmid (h) - sc-403354-ACT from Santa Cruz Biotechnology
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C6orf150 CRISPR Activation Plasmid (h2) - sc-403354-ACT-2 from Santa Cruz Biotechnology
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C6orf150 Lentiviral Activation Particles (h) - sc-403354-LAC from Santa Cruz Biotechnology
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C6orf150 Lentiviral Activation Particles (h2) - sc-403354-LAC-2 from Santa Cruz Biotechnology
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C6orf150 siRNA (h) - sc-95512 from Santa Cruz Biotechnology
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C6orf150 shRNA Plasmid (h) - sc-95512-SH from Santa Cruz Biotechnology
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C6orf150 shRNA (h) Lentiviral Particles - sc-95512-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU015231 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN5033 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND5033 from Sigma-Aldrich
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MB21D1 siRNA (Human) - CRJ4425 from Cohesion Biosciences
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cGAS CRISPR/Cas9 KO Plasmid (h2) - sc-403354-KO-2 from Santa Cruz Biotechnology
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cGAS HDR Plasmid (h2) - sc-403354-HDR-2 from Santa Cruz Biotechnology
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shRNA set against Human MB21D1 (NM_138441.2) - SHH338731 from Creative biogene
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shRNA set against Human C6orf150(NM_138441.2) - SHG127159 from Creative biogene
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MB21D1 shRNA Plasmid (Human) - CHJ4425 from Cohesion Biosciences
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MB21D1 miRNA 3'UTR clone - MiUTR1H-01446 from Creative biogene
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