POLR1E

Gene Symbol POLR1E
Entrez Gene 64425
Alt Symbol PAF53, PRAF1
Species Human
Gene Type protein-coding
Description polymerase (RNA) I polypeptide E, 53kDa
Other Description DNA-directed RNA polymerase I subunit E|DNA-directed RNA polymerase I subunit RPA49|RNA polymerase I subunit A49|RNA polymerase I-associated factor 53|polymerase (RNA) I associated factor 1
Swissprots Q5VZT3 Q96L20 Q8NBA9 Q9GZS1
Accessions EAW58280 EAW58281 Q9GZS1 AK023452 BAB14579 AK024032 BAB14791 AK091294 BAC03629 AK125471 AK225050 AK301347 BAG62895 AK302345 BAG63673 AK303167 BAG64267 BC001337 AAH01337 BC009757 BC014180 BC014331 AAH14331 BC017766 CR457313 CAG33594 XM_005251547 XP_005251604 NM_001282766 NP_001269695 NM_022490 NP_071935
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF (By similarity). {ECO:0000250}.
Subcellular Location Nucleus, nucleolus {ECO:0000269|PubMed:12429849}.
Top Pathways Purine metabolism, RNA polymerase, Pyrimidine metabolism

POLR1E (C-11) - sc-398270 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
POLR1E (C-11): sc-398270. Western blot analysis of POLR1E expression in JAR (A), HeLa (B), K-562 (C) and Jurkat (D) whole cell lysates and HeLa nuclear extract (E).

POLR1E (H-106) - sc-368534 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
POLR1E (H-106): sc-368534. Western blot analysis of POLR1E expression in A549 (A) and A-431 (B) whole cell lysates.

POLR1E (E-14) - sc-107947 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

POLR1E (S-14) - sc-107949 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
POLR1E (S-14): sc-107949. Western blot analysis of POLR1E expression in Jurkat (A) and A549 (B) whole cell lysates.

Anti-PRAF1 antibody - ab88623 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-PRAF1 antibody (ab88623) at 1 µg/ml + U-2 OS at 50 µg

Anti-PRAF1 antibody - ab130572 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-PRAF1 antibody (ab130572) at 1/100 dilution + K562 cell line lysate at 35 µg

Anti-PRAF1 antibody - ab68293 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-PRAF1 antibody (ab68293) at 1/500 dilutionLane 1 : PRAF1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-POLR1E Antibody (aa171-198, AP) - LS-C226980 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POLR1E Antibody (aa171-198, APC) - LS-C226981 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POLR1E Antibody (aa171-198, Biotin) - LS-C226982 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POLR1E Antibody (aa171-198, FITC) - LS-C226983 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POLR1E Antibody (aa171-198, Azide-free, HRP) - LS-C226984 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POLR1E Antibody (aa170-198) - LS-C324166 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POLR1E Antibody (aa171-198, PE) - LS-C226985 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-POLR1E Antibody (aa35-84) - LS-C116719 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
POLR1E / PRAF1 antibody LS-C116719 Western blot of HeLa lysate.

Anti-POLR1E Antibody (aa171-198) - LS-C163847 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
POLR1E Antibody western blot of K562 cell line lysates (35 ug/lane). The POLR1E antibody detected the POLR1E protein (arrow).

PRAF1 Antibody - NBP1-89867 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PRAF1 Antibody [NBP1-89867] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

PRAF1 Antibody - NBP1-56449 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PRAF1 Antibody [NBP1-56449] - Lanes: Lane1: 50 ug mouse heptoma cell lysate Primary Antibody Dilution: 1:1000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:5000 Gene Name: PORL1E

PRAF1 Antibody - H00064425-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: PRAF1 Antibody [H00064425-B01] - analysis of PRAF1 expression in transfected 293T cell line by PRAF1 MaxPab polyclonal antibody (B01). Lane 1: PRAF1 transfected lysate (47.30 KDa). Lane 2: Non-transfected lysate.

PRAF1 Antibody - H00064425-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: PRAF1 Antibody [H00064425-B01P] - Analysis of POLR1E expression in U-2 OS.

PRAF1 Antibody - NBP2-19921 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PRAF1 Antibody [NBP2-19921] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:1000.

Anti-POLR1E antibody produced in rabbit - HPA022527 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-POLR1E: <B>Cat. No. HPA022527</B>: Immunohistochemical staining of human rectum shows nucleolar and cytoplasmic positivity in glandular cells.

Anti-POLR1E, (N-terminal) antibody produced in rabbit - SAB2106152 from Sigma-Aldrich

Prices $347.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-POLR1E (N-Terminal): <B>Cat. No. SAB2106152</B>: POLR1E in Hela cell lysates with POLR1E antibody at 1.0 μg/mL.

POLR1E antibody - N-terminal region (ARP57639_P050) - ARP57639_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

POLR1E-Antibody-Center - AP17099c from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
POLR1E Antibody (Center) (Cat. #AP17099c) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the POLR1E antibody detected the POLR1E protein (arrow).
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