RFK

Gene Symbol RFK
Entrez Gene 55312
Alt Symbol RIFK
Species Human
Gene Type protein-coding
Description riboflavin kinase
Other Description 0610038L10Rik|ATP:riboflavin 5'-phosphotransferase|flavokinase
Swissprots Q969G6 Q9NUT7 Q5JSG9
Accessions EAW62578 EAW62579 Q969G6 AF038172 AK002011 BAA92033 AK123933 AK307079 AK315734 BAG38089 BC007069 AAH07069 BF979572 DA154523 DB499473 DB531307 NM_018339 NP_060809
Function Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase. {ECO:0000269|PubMed:19641494}.
Subcellular Location Cytoplasm {ECO:0000250}.
Tissue Specificity Detected in brain, placenta and urinary bladder.
Top Pathways Riboflavin metabolism

Riboflavin kinase (E-7) - sc-398830 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Riboflavin kinase (E-7): sc-398830. Western blot analysis of Riboflavin kinase expression in non-transfected: sc-117752 (A) and mouse Riboflavin kinase transfected: sc-123131 (B) 293T whole cell lysates.

Riboflavin kinase (H-86) - sc-67308 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Riboflavin kinase (H-86): sc-67308. Western blot analysis of Riboflavin kinase expression in non-transfected: sc-117752 (A) and mouse Riboflavin kinase transfected: sc-123131 (B) 293T whole cell lysates.

Riboflavin kinase (G-20) - sc-66625 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Riboflavin kinase (G-20): sc-66625. Western blot analysis of Riboflavin kinase expression in non-transfected: sc-117752 (A) and mouse Riboflavin kinase transfected: sc-123131 (B) 293T whole cell lysates.

Riboflavin kinase (N-14) - sc-66627 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Riboflavin kinase (N-14): sc-66627. Western blot analysis of Riboflavin kinase expression in HeLa (A) and Jurkat (B) whole cell lysates.

Anti-RFK antibody - ab168450 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-RFK antibody (ab168450) at 1 µg/mlLane 1 : Transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-mouse HRP at 1/2500 dilution

Anti-RFK antibody - ab60214 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab60214 at 1/50 dilution, staining RFK in human breast carcinoma by Immunohistochemistry, Formalin fixed, Paraffin embedded tissue, followed by peroxidase conjugated secondary antibody and AEC staining.

Anti-RFK antibody - ab133974 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-RFK antibody (ab133974) at 1 µg/ml + HepG2 cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbitdeveloped using the ECL technique

Anti-RFK Antibody (aa48-97) - LS-C151786 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
RFK antibody Western Blot of HepG2.

Anti-RFK Antibody (N-Terminus, APC) - LS-C282202 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFK Antibody (N-Terminus, AP) - LS-C282201 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFK Antibody (N-Terminus, Biotin) - LS-C282203 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFK Antibody (N-Terminus, FITC) - LS-C282204 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFK Antibody (N-Terminus, Azide-free, HRP) - LS-C282205 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFK Antibody (N-Terminus, PE) - LS-C282206 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RFK Antibody (N-Terminus) - LS-C328519 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

RFK Antibody - NBP2-37962 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: RFK Antibody [NBP2-37962] - Immunofluorescent staining of human cell line A549 shows localization to the Golgi apparatus.

RFK Antibody - H00055312-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: RFK Antibody [H00055312-B01] - analysis of RFK expression in transfected 293T cell line by RFK MaxPab polyclonal antibody (B01). Lane 1: RFK transfected lysate (18.40 KDa). Lane 2: Non-transfected lysate.

RFK Antibody - H00055312-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal

RFK Antibody - H00055312-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: RFK Antibody [H00055312-B01P] - Analysis of RFK expression in transfected 293T cell line by RFK polyclonal antibody. Lane 1: RFK transfected lysate(17.82 KDa). Lane 2: Non-transfected lysate.

Anti-RFK (C-term) antibody produced in rabbit - SAB1300830 from Sigma-Aldrich

Prices $291.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RFK antibody produced in mouse - SAB1409492 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-RFK: <B>Cat. No. SAB1409492</B>: Antibody concentration: 0.1-5 μg/mL. Specific band of ~17.8 kDa using RFK transfected 293T lysate.<BR/><B>Lanes</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Anti-RFK (71-85) antibody produced in rabbit - R6405 from Sigma-Aldrich

Prices $342.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-RFK (71-85): <B>Cat. No. R6405</B>: Immunofluorescence of HUVEC cells using RFK (71-85) (RB),<B>Cat. No. R6405</B> (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-RFK (N-term) antibody produced in rabbit - SAB1300829 from Sigma-Aldrich

Prices $291.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-RFK (N-term) Antibody: <B>Cat. No. SAB1300829</B>: RFK in human breast carcinoma tissue with Anti-RFK (N-term) Antibody at 40 μg/mL.

RFK Antibody - middle region (ARP64673_P050) - ARP64673_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

RFK-Antibody-N-term - AP7183a from Abgent, a WuXi AppTec company

Prices $99.00, $295.00
Sizes 80 µl, 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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