HCAR2

Gene Symbol	HCAR2
Entrez Gene	338442
Alt Symbol	GPR109A, HCA2, HM74a, HM74b, NIACR1, PUMAG, Puma-g
Species	Human
Gene Type	protein-coding
Description	hydroxycarboxylic acid receptor 2
Other Description	G protein-coupled receptor 109A\|G protein-coupled receptor HM74a\|G-protein coupled receptor 109A\|G-protein coupled receptor HM74A\|hydroxy-carboxylic acid receptor 2\|niacin receptor 1\|nicotinic acid receptor
Swissprots	Q8TDS4 A0PJL5 A7LGG3
Accessions	ABS29270 BAB89345 BAC05891 BAC06094 CAD69365 Q8TDS4 AK290915 BAF83604 AL572062 AL698846 AY148884 AAN71621 BC027965 AAH27965 BC056419 AAH56419 BC063461 CD364466 NM_177551 NP_808219
Function	Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5- methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide. {ECO:0000269\|PubMed:17932499}.
Subcellular Location	Cell membrane {ECO:0000269\|PubMed:17932499}; Multi-pass membrane protein {ECO:0000269\|PubMed:17932499}.
Tissue Specificity	Expression largely restricted to adipose tissue and spleen. Expressed on mature neutrophils but not on immature neutrophils or eosinophils. {ECO:0000269\|PubMed:12522134, ECO:0000269\|PubMed:17932499}.
Top Pathways	cAMP signaling pathway

HM74 (C-18) - sc-48537 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

HM74 (C-18): sc-48537. Western blot analysis of HM74 expression in non-transfected: sc-117752 (A) and human HM74 transfected: sc-112381 (B) 293T whole cell lysates.

Anti-Puma gamma antibody - ab198693 from Abcam

Prices	$359.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling Puma gamma using ab198693 at a 1/40 dilution.

Anti-Puma gamma antibody - ab81825 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-Puma gamma antibody (ab81825) at 1 µg/ml + Liver (Human) Tissue Lysate - adult normal tissue (ab29889) at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Puma gamma antibody [MM0329-6S24] - ab89298 from Abcam

Prices	$370.00
Sizes	50 µg
Host	Rat
Clonality	Monoclonal
Clone	MM0329-6S24

Anti-HCAR2 / NIACR1 Antibody (aa207-236, Biotin) - LS-C238030 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HCAR2 / NIACR1 Antibody (aa207-236, FITC) - LS-C238031 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HCAR2 / NIACR1 Antibody (aa207-236, AP) - LS-C238028 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HCAR2 / NIACR1 Antibody (aa207-236, PE) - LS-C238033 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HCAR2 / NIACR1 Antibody (aa207-236, APC) - LS-C238029 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HCAR2 / NIACR1 Antibody (aa207-236) - LS-C329895 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HCAR2 / NIACR1 Antibody (aa207-236, Azide-free, HRP) - LS-C248913 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-HCAR2 / NIACR1 Antibody (aa200-228) - LS-C164871 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

NIACR1 Antibody western blot of NCI-H292 cell line lysates (35 ug/lane). The NIACR1 antibody detected the NIACR1 protein (arrow).

Anti-Niacin Receptor (PUMAG/NIACR1/ Antibody (aa285-334) - LS-C120604 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Immunofluorescence of MCF7 cells, using GPR109 Antibody. The picture on the right is treated with the synthesized peptide.

HM74A/PUMA-G/GPR109A/NIACR1 Antibody (MM0329-6S24) - NBP2-12358 from Novus Biologicals

Prices	$349.00
Sizes	100 µg
Host	Rat
Clonality	Monoclonal
Clone	MM0329-6S24

HM74A/PUMA-G/GPR109A/NIACR1 Antibody (245106) [Allophycocyanin] - FAB2760A from Novus Biologicals

Prices	$359.00
Sizes	100 tests
Host	Rat
Clonality	Monoclonal
Clone	245106

Human peripheral blood neutrophils were stained with Rat Anti-Human HM74A/PUMA-G/GPR109A APC-conjugated Monoclonal Antibody (Catalog # FAB2760A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

HM74A/PUMA-G/GPR109A/NIACR1 Antibody (245106) [Phycoerythrin] - FAB2760P from Novus Biologicals

Prices	$309.00
Sizes	100 tests
Host	Rat
Clonality	Monoclonal
Clone	245106

Human peripheral blood neutrophils were stained with Rat Anti-Human HM74A/PUMA-G/GPR109A PE-conjugated Monoclonal Antibody (Catalog # FAB2760P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

HM74A/PUMA-G/GPR109A/NIACR1 Antibody - NBP1-92180 from Novus Biologicals

Prices	$419.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunocytochemistry/Immunofluorescence: HM74A/PUMA-G/GPR109A/NIACR1 Antibody [NBP1-92180] - Immunofluorescent staining of human cell line RT4 shows localization to cell junctions.

HM74A/PUMA-G/GPR109A/NIACR1 Antibody (245106) [Fluorescein] - FAB2760F from Novus Biologicals

Prices	$249.00
Sizes	100 tests
Host	Rat
Clonality	Monoclonal
Clone	245106

Human neutrophils were stained with Rat Anti-Human HM74A/PUMA-G/GPR109A Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB2760F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). View our protocol for Staining Membrane-associated Proteins.

HM74A/PUMA-G/GPR109A/NIACR1 Antibody (MM0329-6S24) [DyLight 680] - NBP2-12358FR from Novus Biologicals

Host	Rat
Clonality	Monoclonal
Clone	MM0329-6S24

HM74A/PUMA-G/GPR109A/NIACR1 Antibody (MM0329-6S24) [DyLight 350] - NBP2-12358UV from Novus Biologicals

Host	Rat
Clonality	Monoclonal
Clone	MM0329-6S24

HM74A/PUMA-G/GPR109A/NIACR1 Antibody (MM0329-6S24) [DyLight 405] - NBP2-12358V from Novus Biologicals

Host	Rat
Clonality	Monoclonal
Clone	MM0329-6S24

HM74A/PUMA-G/GPR109A/NIACR1 Antibody (MM0329-6S24) [PE] - NBP2-12358PE from Novus Biologicals

Host	Rat
Clonality	Monoclonal
Clone	MM0329-6S24

HM74A/PUMA-G/GPR109A/NIACR1 Antibody (MM0329-6S24) [7C] - NBP2-12358V2 from Novus Biologicals

Host	Rat
Clonality	Monoclonal
Clone	MM0329-6S24

HM74A/PUMA-G/GPR109A/NIACR1 Antibody (MM0329-6S24) [PerCP] - NBP2-12358PCP from Novus Biologicals

Host	Rat
Clonality	Monoclonal
Clone	MM0329-6S24

HM74A/PUMA-G/GPR109A/NIACR1 Antibody (MM0329-6S24) [DyLight 488] - NBP2-12358G from Novus Biologicals

Host	Rat
Clonality	Monoclonal
Clone	MM0329-6S24

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