HCAR2
| Gene Symbol | HCAR2 |
|---|---|
| Entrez Gene | 338442 |
| Alt Symbol | GPR109A, HCA2, HM74a, HM74b, NIACR1, PUMAG, Puma-g |
| Species | Human |
| Gene Type | protein-coding |
| Description | hydroxycarboxylic acid receptor 2 |
| Other Description | G protein-coupled receptor 109A|G protein-coupled receptor HM74a|G-protein coupled receptor 109A|G-protein coupled receptor HM74A|hydroxy-carboxylic acid receptor 2|niacin receptor 1|nicotinic acid receptor |
| Swissprots | Q8TDS4 A0PJL5 A7LGG3 |
| Accessions | ABS29270 BAB89345 BAC05891 BAC06094 CAD69365 Q8TDS4 AK290915 BAF83604 AL572062 AL698846 AY148884 AAN71621 BC027965 AAH27965 BC056419 AAH56419 BC063461 CD364466 NM_177551 NP_808219 |
| Function | Acts as a high affinity receptor for both nicotinic acid (also known as niacin) and (D)-beta-hydroxybutyrate and mediates increased adiponectin secretion and decreased lipolysis through G(i)-protein-mediated inhibition of adenylyl cyclase. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet. Mediates nicotinic acid-induced apoptosis in mature neutrophils. Receptor activation by nicotinic acid results in reduced cAMP levels which may affect activity of cAMP-dependent protein kinase A and phosphorylation of target proteins, leading to neutrophil apoptosis. The rank order of potency for the displacement of nicotinic acid binding is 5- methyl pyrazole-3-carboxylic acid = pyridine-3-acetic acid > acifran > 5-methyl nicotinic acid = acipimox >> nicotinuric acid = nicotinamide. {ECO:0000269|PubMed:17932499}. |
| Subcellular Location | Cell membrane {ECO:0000269|PubMed:17932499}; Multi-pass membrane protein {ECO:0000269|PubMed:17932499}. |
| Tissue Specificity | Expression largely restricted to adipose tissue and spleen. Expressed on mature neutrophils but not on immature neutrophils or eosinophils. {ECO:0000269|PubMed:12522134, ECO:0000269|PubMed:17932499}. |
| Top Pathways | cAMP signaling pathway |
HM74 CRISPR/Cas9 KO Plasmid (h) - sc-405312 from Santa Cruz Biotechnology
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HM74 HDR Plasmid (h) - sc-405312-HDR from Santa Cruz Biotechnology
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HM74 Double Nickase Plasmid (h) - sc-405312-NIC from Santa Cruz Biotechnology
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HM74 Double Nickase Plasmid (h2) - sc-405312-NIC-2 from Santa Cruz Biotechnology
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HM74 CRISPR Activation Plasmid (h) - sc-405312-ACT from Santa Cruz Biotechnology
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HM74 CRISPR Activation Plasmid (h2) - sc-405312-ACT-2 from Santa Cruz Biotechnology
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HM74 Lentiviral Activation Particles (h) - sc-405312-LAC from Santa Cruz Biotechnology
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HM74 Lentiviral Activation Particles (h2) - sc-405312-LAC-2 from Santa Cruz Biotechnology
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HM74A siRNA (h) - sc-60792 from Santa Cruz Biotechnology
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HM74A shRNA Plasmid (h) - sc-60792-SH from Santa Cruz Biotechnology
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HM74A shRNA (h) Lentiviral Particles - sc-60792-V from Santa Cruz Biotechnology
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HM74A/PUMA-G/GPR109A/NIACR1 RNAi - H00338442-R02 from Novus Biologicals
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CompoZr® Knockout ZFN Kit - CKOZFN9541 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND9541 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR06765 from Sigma-Aldrich
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HCAR2 siRNA (Human) - CRJ7240 from Cohesion Biosciences
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HCAR2 - MBS8219975 from MyBioSource
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shRNA set against Human HCAR2 (NM_177551.3) - SHH310721 from Creative biogene
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shRNA set against Human GPR109A(NM_177551.3) - SHH097103 from Creative biogene
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Human GPR109A siRNA - orb277741 from Biorbyt
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HCAR2 shRNA Plasmid (Human) - CHJ7240 from Cohesion Biosciences
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HCAR2 miRNA 3'UTR clone - MiUTR1H-04175 from Creative biogene
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