ARFGAP3

Gene Symbol ARFGAP3
Entrez Gene 26286
Alt Symbol ARFGAP1
Species Human
Gene Type protein-coding
Description ADP-ribosylation factor GTPase activating protein 3
Other Description ADP-ribosylation factor GTPase activating protein 1|ADP-ribosylation factor GTPase-activating protein 3|ARF GAP 3
Swissprots Q9NT10 Q9BSC6 Q9H9J0 Q9Y4V3 Q9NP61 Q9NUP5 E9PB03 Q9Y4V4
Accessions EAW73275 EAW73276 Q9NP61 AF111847 AAF40310 AK002083 BAA92076 AK022768 BAB14236 AK127525 AK297635 BAH12636 AK300531 BAH13298 AK307967 AK315720 AL137598 CAB70834 AL159143 CAB76901 BC005122 AAH05122 BC041383 BI547713 BQ019944 CA431867 CR456382 CAG30268 CU012940 CAK54371 CU013228 CAK54670 DB458974 DQ893007 ABM83933 DQ896253 ABM87252 XM_005261525 XP_005261582 NM_001142293 NP_001135765 NM_014570 NP_055385
Function GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes. {ECO:0000269|PubMed:11172815}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:17760859}. Golgi apparatus membrane {ECO:0000269|PubMed:17760859}; Peripheral membrane protein {ECO:0000269|PubMed:17760859}; Cytoplasmic side {ECO:0000269|PubMed:17760859}. Note=Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment.
Tissue Specificity Widely expressed. Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary. {ECO:0000269|PubMed:10704287}.
Top Pathways Endocytosis

ARFGAP1/3 (D-8) - sc-365418 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ARFGAP1/3 (D-8): sc-365418. Western blot analysis of ARFGAP1/3 expression in HeLa whole cell lysate.

ARFGAP1/3 (G-11) - sc-374328 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ARFGAP1/3 (G-11): sc-374328. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

ARFGAP1/2/3 (F-3) - sc-390955 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ARFGAP1/2/3 (F-3): sc-390955. Western blot analysis of ARFGAP1/2/3 expression in HeLa (A) and ZR-75-1 (B) whole cell lysates.

ARFGAP1/2/3 (H-84) - sc-292277 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ARFGAP1/2/3 (H-84): 292277. Western blot analysis of ARFGAP1/2/3 expression in mouse kidney tissue extract.

ARFGAP1/3 (N-20) - sc-46784 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ARFGAP1/3 (N-20): sc-46784. Western blot analysis of ARFGAP3 expression in HeLa whole cell lysate (A) and mouse testis tissue extract (B).

ARFGAP1/3 (C-15) - sc-46779 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-ARFGAP3 antibody - ab85971 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ARFGAP3 antibody (ab85971) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µg

Anti-ARFGAP3 antibody - ab181632 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ARFGAP3 antibody (ab181632) at 1/1000 dilutionLane 1 : HeLa whole cell lysateLane 2 : HeLa whole cell lysate siRNA treatedLysates/proteins at 10 µg per lane.SecondaryIRDye800™ anti-rabbit at 1/10000 dilution

Anti-ARFGAP3 antibody - ab1308 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ARFGAP3 staining (2µg/ml) of Mouse Brain extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence

Anti-ARFGAP3 antibody - ab122987 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-ARFGAP3 antibody (ab122987) at 0.125 µg/ml + Human fetal lung lysate at 10 µg

Anti-ARFGAP3 antibody - C-terminal - ab153811 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-ARFGAP3 antibody - C-terminal (ab153811) at 1/500 dilution + 293T whole cell lysate at 30 µg

Anti-ARFGAP3 Antibody (aa445-473) - LS-C156455 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ARFGAP3 Antibody western blot of T47D cell line lysates (35 ug/lane). The ARFGAP3 antibody detected the ARFGAP3 protein (arrow).

Anti-ARFGAP3 Antibody (N-Terminus) - LS-C155439 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Sample (30 ug of whole cell lysate) A: A549 B: HeLa 7.5% SDS PAGE ARFGAP3 antibody diluted at 1:1000

Anti-ARFGAP3 Antibody - LS-C209178 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescence Microscopy of Rabbit Anti-ArfGAP3 Antibody. Tissue: HeLa Whole Cell. Fixation: MeOH. Antigen retrieval: not required. Primary antibody: ArfGAP3 antibody at 1:100 for 1 h at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ArfGAP3 is cytoplasmic. Staining: ArfGAP3 as green fluorescent signal.

Anti-ARFGAP3 Antibody (aa466-516) - LS-C288790 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human ArfGAP3 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-ArfGAP3 antibody used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

Anti-ARFGAP3 Antibody (aa777-787) - LS-C121389 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-ARFGAP3 Antibody (aa445-473, AP) - LS-C232796 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARFGAP3 Antibody (aa445-473, APC) - LS-C232797 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARFGAP3 Antibody (aa445-473, Biotin) - LS-C232798 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARFGAP3 Antibody (aa91-140) - LS-C145570 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ARFGAP3 antibody LS-C145570 Western blot of 721_B Cell lysate. Antibody concentration 1 ug/ml.

Anti-ARFGAP3 Antibody (aa377-426) - LS-C145995 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ARFGAP3 antibody LS-C145995 Western blot of Fetal Lung lysate. Antibody concentration 1 ug/ml.

Anti-ARFGAP3 Antibody (aa445-473, Azide-free, HRP) - LS-C249450 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARFGAP3 Antibody (aa445-473, PE) - LS-C232804 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-ARFGAP3 Antibody (aa504-516) - LS-C54528 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody staining (2 ug/ml) of mouse brain extracts (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Anti-ARFGAP3 Antibody (aa445-473, FITC) - LS-C232799 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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