Gene Symbol | ARHGEF1 |
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Entrez Gene | 9138 |
Alt Symbol | GEF1, LBCL2, LSC, P115-RHOGEF, SUB1.5 |
Species | Human |
Gene Type | protein-coding |
Description | Rho guanine nucleotide exchange factor (GEF) 1 |
Other Description | 115 kDa guanine nucleotide exchange factor|115-kD protein|Lsc homolog|p115RhoGEF|rho guanine nucleotide exchange factor 1 |
Swissprots | Q96F17 Q6NX52 Q8N4J4 Q9BSB1 O00513 Q92888 Q96BF4 |
Accessions | EAW57077 EAW57078 EAW57079 EAW57080 EAW57081 EAW57082 EAW57083 EAW57084 Q92888 AK026781 AL832294 BC005155 AAH05155 BC011726 AAH11726 BC015652 BC034013 AAH34013 BC035127 AAH35127 BC064996 BC067262 AAH67262 BM147791 BQ944132 BT007421 AAP36089 DQ893592 ABM84518 DQ895004 ABM85930 EU832644 ACE87735 EU832721 ACE87053 U64105 AAB17896 XM_005259386 XP_005259443 XM_005259387 XP_005259444 XM_005259388 XP_005259445 XM_005259389 XP_005259446 XM_005259390 XP_005259447 XM_006723463 XP_006723526 XM_011527468 XP_011525770 NM_004706 NP_004697 NM_198977 NP_945328 NM_199002 NP_945353 |
Function | Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2- induced RhoA activation. {ECO:0000269|PubMed:20098430, ECO:0000269|PubMed:8810315, ECO:0000269|PubMed:9641915, ECO:0000269|PubMed:9641916}. |
Subcellular Location | Cytoplasm {ECO:0000269|PubMed:10747909}. Membrane {ECO:0000269|PubMed:10747909}. Note=Translocated to the membrane by activated GNA13 or LPA stimulation. |
Tissue Specificity | Ubiquitously expressed. {ECO:0000269|PubMed:8810315, ECO:0000269|PubMed:9135076}. |
Top Pathways | Vascular smooth muscle contraction, Proteoglycans in cancer, Regulation of actin cytoskeleton |
RhoGEF p115 CRISPR/Cas9 KO Plasmid (h) - sc-402495 from Santa Cruz Biotechnology
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RhoGEF p115 HDR Plasmid (h) - sc-402495-HDR from Santa Cruz Biotechnology
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RhoGEF p115 Double Nickase Plasmid (h) - sc-402495-NIC from Santa Cruz Biotechnology
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RhoGEF p115 Double Nickase Plasmid (h2) - sc-402495-NIC-2 from Santa Cruz Biotechnology
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RhoGEF p115 CRISPR Activation Plasmid (h) - sc-402495-ACT from Santa Cruz Biotechnology
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RhoGEF p115 CRISPR Activation Plasmid (h2) - sc-402495-ACT-2 from Santa Cruz Biotechnology
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RhoGEF p115 Lentiviral Activation Particles (h) - sc-402495-LAC from Santa Cruz Biotechnology
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RhoGEF p115 Lentiviral Activation Particles (h2) - sc-402495-LAC-2 from Santa Cruz Biotechnology
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RhoGEF p115 siRNA (h) - sc-41734 from Santa Cruz Biotechnology
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RhoGEF p115 shRNA Plasmid (h) - sc-41734-SH from Santa Cruz Biotechnology
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RhoGEF p115 shRNA (h) Lentiviral Particles - sc-41734-V from Santa Cruz Biotechnology
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ARHGEF1 RNAi - H00009138-R01 from Novus Biologicals
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ARHGEF1 RNAi - H00009138-R02 from Novus Biologicals
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ARHGEF1 RNAi - H00009138-R03 from Novus Biologicals
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MISSION® esiRNA - EHU133091 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN3441 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND3441 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR02534 from Sigma-Aldrich
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ARHGEF1 siRNA (Human) - CRH6044 from Cohesion Biosciences
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ARHGEF1 - MBS8219554 from MyBioSource
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shRNA set against Human ARHGEF1 (NM_004706.3) - SHH239674 from Creative biogene
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shRNA set against Human ARHGEF1(NM_004706.3) - SHG070543 from Creative biogene
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shRNA set against Human ARHGEF1(NM_198977.1) - SHG070561 from Creative biogene
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shRNA set against Human ARHGEF1(NM_199002.1) - SHG070621 from Creative biogene
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shRNA set against Human ARHGEF1(NM_198977.1) - SHG070657 from Creative biogene
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