RHEB

Gene Symbol RHEB
Entrez Gene 6009
Alt Symbol RHEB2
Species Human
Gene Type protein-coding
Description Ras homolog enriched in brain
Other Description GTP-binding protein Rheb|Ras homolog enriched in brain 2
Swissprots Q99444 B3KWN6 D3DX13 Q53Y56 Q15382
Accessions AAD15548 CDL93493 EAL24515 EAW53986 EAW53987 EAW53988 EAW53989 Q15382 AF148645 AAF73125 AF493921 AAM12635 AK125446 BAG54198 AK289370 BAF82059 BC009638 BC016155 AAH16155 BC066307 AAH66307 BC107705 AAI07706 BG702213 BM670621 BT006958 AAP35604 D78132 BAA11211 DA475169 Z29677 CAA82774 XM_011516457 XP_011514759 NM_005614 NP_005605
Function Activates the protein kinase activity of mTORC1, and thereby plays a role in the regulation of apoptosis. Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Has low intrinsic GTPase activity. {ECO:0000269|PubMed:12271141, ECO:0000269|PubMed:12869586, ECO:0000269|PubMed:15340059, ECO:0000269|PubMed:15854902, ECO:0000269|PubMed:16098514, ECO:0000269|PubMed:20381137}.
Subcellular Location Endomembrane system {ECO:0000269|PubMed:22002721, ECO:0000305}; Lipid-anchor {ECO:0000269|PubMed:22002721, ECO:0000305}; Cytoplasmic side {ECO:0000269|PubMed:15489334, ECO:0000305}. Golgi apparatus membrane {ECO:0000269|PubMed:22002721}; Lipid-anchor {ECO:0000305|PubMed:22002721}; Cytoplasmic side {ECO:0000305|PubMed:22002721}. Cytoplasm, cytosol {ECO:0000269|PubMed:22002721}. Endoplasmic reticulum membrane {ECO:0000269|PubMed:22002721}; Lipid-anchor {ECO:0000269|PubMed:22002721}; Cytoplasmic side {ECO:0000269|PubMed:22002721}.
Tissue Specificity Ubiquitous. Highest levels observed in skeletal and cardiac muscle. {ECO:0000269|PubMed:8543055}.
Top Pathways Insulin signaling pathway, PI3K-Akt signaling pathway, AMPK signaling pathway, Choline metabolism in cancer, mTOR signaling pathway

Rheb (E1G1R) Rabbit mAb - 13879 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone E1G1R
Western blot analysis of extracts from various cell lines using Rheb (E1G1R) Rabbit mAb.

Rheb Antibody - 4935 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Rheb (B-12) - sc-271509 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rheb (B-12): sc-271509. Western blot analysis of Rheb expression in non-transfected 293T: sc-117752 (A), mouse Rheb transfected 293T: sc-123114 (B) and SH-SY5Y (C) whole cell lysates.

Rheb (C-19) - sc-6341 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rheb (C-19): sc-6341. Western blot analysis of Rheb expression in SK-N-SH (A) and SH-SY5Y (B) whole cell lysates.

Rheb (H-70) - sc-33205 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Rheb (H-70): sc-33205. Western blot analysis of Rheb expression in mouse brain tissue extract.

Rheb (N-19) - sc-6342 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Rheb (N-19): sc-6342. Western blot analysis of Rheb expression in non-transfected: sc-117752 (A) and mouse Rheb transfected: sc-123114 (B) 293T whole cell lysates.

Rheb (80-R) - sc-130398 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Rheb (80-R): sc-130398. Western blot analysis of Rheb expression in Jurkat whole cell lysate.

Anti-RHEB antibody - ab25873 from Abcam

Prices $384.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-RHEB antibody - ab180838 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-RHEB antibody (ab180838) at 1/500 dilutionLane 1 : brain cells lysateLane 2 : HeLa cell lysate

Anti-RHEB antibody - ab76629 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-RHEB antibody (ab76629) at 1 µg/ml + Recombinant tagged full length human RHEB at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

Anti-RHEB antibody - ab25976 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-RHEB antibody (ab25976) at 2 µg/mlLane 2 : Anti-RHEB antibody (ab25976) at 4 µg/mlLane 1 : Rat heart cell lysateLane 2 : Rat heart cell lysate

Anti-RHEB antibody [3H6] (HRP) - ab187825 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 3H6

Anti-RHEB antibody [EPR2971] - ab92313 from Abcam

Prices $387.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2971
Overlay histogram showing Raji cells stained with ab92313 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92313, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Raji cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-RHEB antibody [Rheb 3H6] - ab97894 from Abcam

Prices $372.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone Rheb 3H6

Anti-RHEB antibody [Rheb 5D5] - ab97896 from Abcam

Prices $379.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone Rheb 5D5
LLC-PK1 cells were grown on Transwell cell culture inserts, serum-starved overnight, and then kept in the absence (a) or presence (b) of 20.0 mg/ml albumin for 20 minutes. Blue (DAPI) - nuclei, Green - RAPTOR, Red - RHEB labelled with ab97896. Scale bar = 5 μm.

Anti-RHEB Antibody (aa155-169) - LS-C75448 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RHEB Antibody - LS-C194037 from LifeSpan Bioscience

Prices $905.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-RHEB Antibody (Asn50) IHC-plus™ - LS-B4945 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-RHEB antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4945 dilution 1:100.

Anti-RHEB Antibody (N-Terminus) - LS-C19504 from LifeSpan Bioscience

Prices $325.00, $890.00
Sizes 100 µg, 500 µg
Host Rabbit
Clonality Polyclonal
Western blot of Rheb in rat heart cell lysate with Rheb antibody at (A) 2 and (B) 4 ug/ml.

Anti-RHEB Antibody - LS-C192419 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of RHEB pAb in extracts from mouse brain tissue and Hela cells.

Anti-RHEB Antibody (aa104-134) - LS-C162588 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
RHEB Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human skeletal muscle followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-RHEB Antibody (Internal, clone EPR2971) - LS-C105583 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2971
IHC of paraffin-embedded human brain tissue using anti-Rheb antibody (LS-C105583).

Anti-RHEB Antibody - LS-C331313 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded human liver cancer using RHEB antibody at dilution of 1:200 (x400 lens)

Anti-RHEB Antibody (clone 2C11) - LS-C139165 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 2C11
RHEB monoclonal antibody (M01), clone 2C11. Western blot of RHEB expression in Jurkat.

Anti-RHEB Antibody (aa107-156) IHC-plus™ - LS-B11962 from LifeSpan Bioscience

Prices $450.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)
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