HCST

Gene Symbol HCST
Entrez Gene 10870
Alt Symbol DAP10, KAP10, PIK3AP
Species Human
Gene Type protein-coding
Description hematopoietic cell signal transducer
Other Description DNAX-activation protein 10|kinase assoc pro of ~10kDa|kinase assoc protein|membrane protein DAP10|phosphoinositide-3-kinase adaptor protein|transmembrane adapter protein KAP10
Swissprots Q9UBK5 Q9Y3Y0 Q9UBS1
Accessions AAD46987 Q9UBK5 AF072844 AAD46986 AF122904 AAD47911 AF172929 AAD50293 AF285447 AAG29425 AL050163 CAB43303 AY359058 AAQ89417 BC035931 AAH35931 BC046348 AAH46348 BC065224 AAH65224 NM_001007469 NP_001007470 NM_014266 NP_055081
Function Transmembrane adapter protein which associates with KLRK1 to form an activation receptor KLRK1-HCST in lymphoid and myeloid cells; this receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs); these ligands are up-regulated by stress conditions and pathological state such as viral infection and tumor transformation. Functions as docking site for PI3-kinase PIK3R1 and GRB2. Interaction of ULBPs with KLRK1-HCST triggers calcium mobilization and activation of the PIK3R1, MAP2K/ERK, and JAK2/STAT5 signaling pathways. Both PIK3R1 and GRB2 are required for full KLRK1-HCST-mediated activation and ultimate killing of target cells. In NK cells, KLRK1-HCST signaling directly induces cytotoxicity and enhances cytokine production initiated via DAP12/TYROBP-associated receptors. In T-cells, it provides primarily costimulation for TCR-induced signals. KLRK1-HCST rece
Subcellular Location Membrane {ECO:0000269|PubMed:10426994}; Single-pass type I membrane protein {ECO:0000269|PubMed:10426994}.
Tissue Specificity Predominantly expressed in hemopoietic cells such as NK cells, subset of T-cells and monocytes. Detected in leukocytes, spleen, and thymus. {ECO:0000269|PubMed:10528161}.
Top Pathways Natural killer cell mediated cytotoxicity

DAP10 (C-1) - sc-374038 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DAP10 (C-1): sc-374038. Western blot analysis of DAP10 expression in non-transfected (A) and human DAP10 transfected (B) CMV (transcript variant 1) whole cell lysates.

DAP10 (C-20) - sc-10531 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
DAP10 (C-20): sc-10531. Western blot analysis of DAP10/NKG2-D complex expression in human PBL whole cell lysate.

DAP10 (C-5) - sc-374037 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DAP10 (C-5): sc-374037. Western blot analysis of DAP10 expression in non-transfected (A) and human DAP10 transfected (B) CMV (transcript variant 1) whole cell lysates.

DAP10 (H-3) - sc-374196 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DAP10 (H-3): sc-374196. Western blot analysis of DAP10 expression in non-transfected: sc-117752 (A) and human DAP10 transfected: sc-116977 (B) 293T whole cell lysates.

DAP10 (H-2) - sc-133173 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DAP10 (H-2): sc-133173. Western blot analysis of DAP10 expression in non-transfected: sc-117752 (A) and human DAP10 transfected: sc-116977 (B) 293T whole cell lysates.

DAP10 (FL-93) - sc-25623 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
DAP10 (FL-93): sc-25623. Western blot analysis of DAP10 expression in non-transfected: sc-117752 (A) and human DAP10 transfected: sc-116977 (B) 293T whole cell lysates.

DAP10 (N-17) - sc-10529 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
DAP10 (N-17): sc-10529. Western blot analysis of human recombinant DAP10.

Anti-HCST antibody - ab58596 from Abcam

Prices $370.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-HCST antibody (ab58596) at 0.2 µg/ml + Human spleen lysate at 35 µg

Anti-DAP10 Antibody (aa76-88) - LS-C54702 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Antibody (0.2 ug/ml) staining of human spleen lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HCST Antibody - NB100-55403 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: HCST Antibody [NB100-55403] - (0.2ug/ml) staining of human spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HCST Polyclonal Antibody - bs-15430R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Paraformaldehyde-fixed, paraffin embedded rat spleen tissue; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HCST Polyclonal Antibody, Unconjugated (bs-15430R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Anti-DAP10/HCST antibody produced in goat - SAB2500292 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-DAP10 / HCST: <B>Cat. No. SAB2500292</B>: (0.2 μg/mL) staining of human spleen lysate (35 μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

DAP10--HCST-Antibody - AF1299a from Abgent, a WuXi AppTec company

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
AF1299a (0.2 µg/ml) staining of human spleen lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

DAP10 / HCST antibody, C-term - GTX89210 from GeneTex

Prices $319.00
Sizes 100 μg
Host Goat
Clonality Polyclonal
Anti-DAP10 / HCST antibody, C-term used in Western blot. GTX89210

HCST antibody - orb19379 from Biorbyt

Prices $408.00
Sizes 100 μg
Host Goat
Clonality Polyclonal
orb19379 (0.2ug/ml) staining of human spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Goat Anti-DAP10 / HCST Antibody (OAEB01186) - OAEB01186 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
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