| Gene Symbol | HCST |
|---|---|
| Entrez Gene | 10870 |
| Alt Symbol | DAP10, KAP10, PIK3AP |
| Species | Human |
| Gene Type | protein-coding |
| Description | hematopoietic cell signal transducer |
| Other Description | DNAX-activation protein 10|kinase assoc pro of ~10kDa|kinase assoc protein|membrane protein DAP10|phosphoinositide-3-kinase adaptor protein|transmembrane adapter protein KAP10 |
| Swissprots | Q9UBK5 Q9Y3Y0 Q9UBS1 |
| Accessions | AAD46987 Q9UBK5 AF072844 AAD46986 AF122904 AAD47911 AF172929 AAD50293 AF285447 AAG29425 AL050163 CAB43303 AY359058 AAQ89417 BC035931 AAH35931 BC046348 AAH46348 BC065224 AAH65224 NM_001007469 NP_001007470 NM_014266 NP_055081 |
| Function | Transmembrane adapter protein which associates with KLRK1 to form an activation receptor KLRK1-HCST in lymphoid and myeloid cells; this receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs); these ligands are up-regulated by stress conditions and pathological state such as viral infection and tumor transformation. Functions as docking site for PI3-kinase PIK3R1 and GRB2. Interaction of ULBPs with KLRK1-HCST triggers calcium mobilization and activation of the PIK3R1, MAP2K/ERK, and JAK2/STAT5 signaling pathways. Both PIK3R1 and GRB2 are required for full KLRK1-HCST-mediated activation and ultimate killing of target cells. In NK cells, KLRK1-HCST signaling directly induces cytotoxicity and enhances cytokine production initiated via DAP12/TYROBP-associated receptors. In T-cells, it provides primarily costimulation for TCR-induced signals. KLRK1-HCST rece |
| Subcellular Location | Membrane {ECO:0000269|PubMed:10426994}; Single-pass type I membrane protein {ECO:0000269|PubMed:10426994}. |
| Tissue Specificity | Predominantly expressed in hemopoietic cells such as NK cells, subset of T-cells and monocytes. Detected in leukocytes, spleen, and thymus. {ECO:0000269|PubMed:10528161}. |
| Top Pathways | Natural killer cell mediated cytotoxicity |
DAP10 CRISPR/Cas9 KO Plasmid (h) - sc-402426 from Santa Cruz Biotechnology
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DAP10 HDR Plasmid (h) - sc-402426-HDR from Santa Cruz Biotechnology
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DAP10 Double Nickase Plasmid (h) - sc-402426-NIC from Santa Cruz Biotechnology
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DAP10 Double Nickase Plasmid (h2) - sc-402426-NIC-2 from Santa Cruz Biotechnology
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DAP10 CRISPR Activation Plasmid (h) - sc-402426-ACT from Santa Cruz Biotechnology
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DAP10 CRISPR Activation Plasmid (h2) - sc-402426-ACT-2 from Santa Cruz Biotechnology
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DAP10 Lentiviral Activation Particles (h) - sc-402426-LAC from Santa Cruz Biotechnology
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DAP10 Lentiviral Activation Particles (h2) - sc-402426-LAC-2 from Santa Cruz Biotechnology
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DAP10 siRNA (h) - sc-35171 from Santa Cruz Biotechnology
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DAP10 shRNA Plasmid (h) - sc-35171-SH from Santa Cruz Biotechnology
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DAP10 shRNA (h) Lentiviral Particles - sc-35171-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU122191 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN9931 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND9931 from Sigma-Aldrich
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HCST siRNA (Human) - CRH7403 from Cohesion Biosciences
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HCST - MBS8222041 from MyBioSource
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shRNA set against Human Hc (NM_014266.3) - SHH310705 from Creative biogene
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shRNA set against Human HCST(NM_001007469.1) - SHH116039 from Creative biogene
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shRNA set against Human HCST(NM_014266.3) - SHH116075 from Creative biogene
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Human HCST siRNA - orb267905 from Biorbyt
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HCST shRNA Plasmid (Human) - CHH7403 from Cohesion Biosciences
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HCST miRNA 3'UTR clone - MiUTR1H-04411 from Creative biogene
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HCST miRNA 3'UTR clone - MiUTR1H-04412 from Creative biogene
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