ASAP2

Gene Symbol ASAP2
Entrez Gene 8853
Alt Symbol AMAP2, CENTB3, DDEF2, PAG3, PAP, Pap-alpha, SHAG1
Species Human
Gene Type protein-coding
Description ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
Other Description PYK2 C terminus-associated protein|arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2|centaurin, beta 3|development and differentiation enhancing factor 2|development and differentiation-enhancing factor 2|paxillin-associated protein with ARF GAP activity 3|pyk2 C-terminus-associated protein
Swissprots D6W4Y8 O43150
Accessions AAY14856 AAY14904 AAY24112 EAX01002 EAX01003 EAX01004 EAX01005 EAX01006 O43150 AA918883 AB007860 BAA23696 AK091121 BAC03588 AK123185 AK124900 BC063308 AAH63308 XM_006711898 XP_006711961 XM_006711901 XP_006711964 XM_006711902 XP_006711965 XM_011510403 XP_011508705 XM_011510404 XP_011508706 XM_011510405 XP_011508707 XM_011510406 XP_011508708 XM_011510407 XP_011508709 XM_011510408 XP_011508710 XM_011510409 XP_011508711 NM_001135191 NP_001128663 NM_003887 NP_003878
Function Activates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration. {ECO:0000269|PubMed:10022920, ECO:0000269|PubMed:10749932, ECO:0000269|PubMed:11304556}.
Subcellular Location Cytoplasm. Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein. Note=Colocalizes with F-actin and ARF6 in phagocytic cups.
Tissue Specificity Detected in heart, brain, placenta, kidney, monocytes and pancreas. {ECO:0000269|PubMed:10022920}.
Top Pathways Fc gamma R-mediated phagocytosis, Endocytosis

DDEF2 (C-9) - sc-374323 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
DDEF2 (C-9): sc-374323. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and membrane localization.

DDEF2 (H-300) - sc-15357 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
DDEF2 (H-300): sc-15357. Immunofluorescence staining of normal mouse intestine frozen section showing membrane staining.

DDEF2 (N-18) - sc-11539 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
DDEF2 (N-18): sc-11539. Western blot analysis of DDEF2 expression in KNRK whole cell lysate.

DDEF2 (V-16) - sc-11541 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
DDEF2 (V-16): sc-11541. Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes.

Anti-PAG3 (phospho Y724) antibody - ab18031 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-PAG3 antibody - ab200681 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PAG3 antibody (ab200681)Lane 1 : HEK293T whole cell lysateLane 2 : sp2/0 whole cell lysateLane 3 : H9C2 whole cell lysate

Anti-PAG3 antibody - ab117760 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PAG3 antibody (ab117760) at 0.1 µg/mlLane 1 : Hela whole cell lysate at 50 µgLane 2 : Hela whole cell lysate at 15 µgLane 3 : 293T whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-PAG3 antibody - ab169603 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-PAG3 antibody (ab169603) at 1/500 dilutionLane 1 : PAG3 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique

Anti-PAG3 antibody - N-terminal - ab137514 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PAG3 antibody - N-terminal (ab137514) at 1/1000 dilutionLane 1 : A431 whole cell lysate Lane 2 : Raji whole cell lysate Lysates/proteins at 30 µg per lane.

Anti-ASAP2 / DDEF2 Antibody (phospho-Tyr724) - LS-C38032 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-ASAP2 / DDEF2 Antibody (N-Terminus) - LS-C345175 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of ASAP2 Antibody - N-terminal region with human MCF7 cells lysate.

Anti-ASAP2 / DDEF2 Antibody (aa700-750) - LS-C148569 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of ASAP2 in human brain lysate using 1) preimmune sera at 1:5000 and 2) protein A purified LS-C148569 at 1.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.

Anti-ASAP2 / DDEF2 Antibody (aa900-950) - LS-C289997 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human ASAP2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ASAP2 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. ASAP2 was also immunoprecipitated by rabbit anti-ASAP2 antibody BL11866, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

PAG3 Antibody - NBP2-24614 from Novus Biologicals

Prices $99.00, $229.00
Sizes 25 µg, 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: PAG3 Antibody [NBP2-24614] - Analysis of ASAP2 in human brain lysate using 1) preimmune sera at 1:5000 and 2) protein A purified this antibody. 1.0 ug/ml.

PAG3 Antibody - NBP2-19678 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PAG3 Antibody [NBP2-19678] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.

PAG3 Antibody - NBP1-78214 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PAG3 Antibody [NBP1-78214] - Whole cell lysate from HeLa (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Affinity purified rabbit anti-ASAP2 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. ASAP2 was also immunoprecipitated by another rabbit anti-ASAP2 antibody which recognizes an upstream epitope.

PAG3 Antibody - H00008853-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: PAG3 Antibody [H00008853-A01] - DDEF2 polyclonal antibody (A01), Lot # 060501JCS1 Western Blot analysis of DDEF2 expression in SJCRH30 ( Cat # L027V1 ).

PAG3 Antibody - H00008853-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: PAG3 Antibody [H00008853-B01] - analysis of DDEF2 expression in transfected 293T cell line by DDEF2 MaxPab polyclonal antibody (B01). Lane1: DDEF2 transfected lysate (111.70 KDa). Lane2: Non-transfected lysate.

Anti-ASAP2 (712-725) antibody produced in rabbit - SAB1104238 from Sigma-Aldrich

Prices $285.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells was separated on SDS-PAGE, blotted with Anti-ASAP2 (712-725) (<B>Cat. No. SAB1104238</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500

Anti-ASAP2 (N-terminal) antibody produced in rabbit - SAB2700509 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-ASAP2 (N-terminal): <B>Cat. No. SAB2700509</B>: Western Blot analysis of ASAP2 in extracts from <B>Lanes</B><BR/>A. A431 <BR/>B. Raji using <B>Cat. No. SAB2700509</B> at a 1:1000 dilution.

ASAP2 Antibody - N-terminal region (ARP67972_P050) - ARP67972_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

DDEF2 antibody [N1N2], N-term - GTX115146 from GeneTex

Prices $119.00
Sizes 25 μl
Host Rabbit
Clonality Polyclonal
Anti-DDEF2 antibody [N1N2], N-term used in Western blot. GTX115146

PAG3 (phospho Tyr724) antibody - GTX18031 from GeneTex

Prices $319.00
Sizes 50 μl
Host Rabbit
Clonality Polyclonal
Anti-PAG3 (phospho Tyr724) antibody used in Western blot. GTX18031

DDEF2 Antibody - abx121077 from Abbexa

Host Rabbit
Clonality Polyclonal
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