CPSF3

Gene Symbol CPSF3
Entrez Gene 51692
Alt Symbol CPSF-73, CPSF73
Species Human
Gene Type protein-coding
Description cleavage and polyadenylation specific factor 3, 73kDa
Other Description cleavage and polyadenylation specificity factor subunit 3|mRNA 3'-end-processing endonuclease CPSF-73
Swissprots Q53RS2 Q96F36 O14769 Q9UKF6
Accessions AAY14858 EAX00989 EAX00990 Q9UKF6 AA635764 AF017269 AAB70268 AF171877 AAF00224 AK025440 AK223487 BAD97207 AK298918 BAG61024 BC011654 AAH11654 BC014106 BC020211 AAH20211 BC030988 AAH30988 BC043432 DC420903 EF036508 ABO65094 EU176402 ABW03853 XM_005246167 XP_005246224 XM_005246168 XP_005246225 XM_011510362 XP_011508664 NM_016207 NP_057291
Function Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP- dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete remov
Subcellular Location Nucleus {ECO:0000269|PubMed:15037765}.
Top Pathways mRNA surveillance pathway

CPSF3 (C-20) - sc-26662 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

CPSF3 (C-3) - sc-393001 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CPSF3 (C-3): sc-393001. Western blot analysis of CPSF3 expression in HeLa (A), Hep G2 (B) and NIH/3T3 (C) whole cell lysates.

CPSF3 (H-145) - sc-292505 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

CPSF3 (T-20) - sc-26661 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-CPSF73 antibody - ab57205 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-CPSF73 antibody [EPR7468] - ab131245 from Abcam

Prices $99.00
Sizes 10 µl
Host Rabbit
Clonality Monoclonal
Clone EPR7468
All lanes : Anti-CPSF73 antibody [EPR7468] (ab131245) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : Jurkat cell lysateLane 4 : Caco-2 cell lysateLane 5 : RAW 264.7 cell lysateLane 6 : PC-12 cell lysateLane 7 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-CPSF3 / CPSF Antibody (aa667-717) - LS-C63946 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-CPSF3 / CPSF Antibody (aa1-50) - LS-C79861 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-CPSF3 / CPSF Antibody (aa634-684) - LS-C79862 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-CPSF3 / CPSF Antibody (aa189-238) - LS-C31377 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
A: Marker, B: Jurkat Cell Lysate

Anti-CPSF3 / CPSF Antibody (aa191-221, AP) - LS-C234179 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-CPSF3 / CPSF Antibody (aa585-685, clone 10B2590, Azide-free) - LS-C183535 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 10B2590

Anti-CPSF3 / CPSF Antibody (C-Terminus) - LS-C31378 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
CPSF3 antibody LS-C31378 was used in IHC to stain formalin-fixed, paraffin-embedded human liver.

Anti-CPSF3 / CPSF Antibody (aa191-221, Biotin) - LS-C234181 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-CPSF3 / CPSF Antibody (aa191-221, APC) - LS-C234180 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-CPSF3 / CPSF Antibody (aa191-221, FITC) - LS-C234182 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-CPSF3 / CPSF Antibody (aa191-221, PE) - LS-C234187 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-CPSF3 / CPSF Antibody (aa191-221, Azide-free, HRP) - LS-C249743 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-CPSF3 / CPSF Antibody - LS-C309980 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-CPSF3 / CPSF Antibody (aa191-221) - LS-C320568 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-CPSF3 / CPSF Antibody - LS-C307076 from LifeSpan Bioscience

Host Mouse
Clonality Polyclonal

Anti-CPSF3 / CPSF Antibody (Internal, clone EPR7468) - LS-C154497 from LifeSpan Bioscience

Host Rabbit
Clonality Monoclonal
Clone EPR7468
Immunofluorescent staining of HeLa cells using anti-CPSF3 antibody.

Anti-CPSF3 / CPSF Antibody (aa634-684) IHC-plus™ - LS-B11044 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Anti-CPSF3 / CPSF antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B11044 dilution 1:100.

Anti-CPSF3 / CPSF Antibody (aa191-221) - LS-C167003 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
CPSF3 Antibody western blot of A375 cell line lysates (35 ug/lane). The CPSF3 antibody detected the CPSF3 protein (arrow).

Anti-CPSF3 / CPSF Antibody (clone 1H8) - LS-C139136 from LifeSpan Bioscience

Host Mouse
Clonality Monoclonal
Clone 1H8
CPSF3 monoclonal antibody (M06), clone 1H8. Western blot of CPSF3 expression in Raw 264.7.
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