CPSF3
| Gene Symbol | CPSF3 |
|---|---|
| Entrez Gene | 51692 |
| Alt Symbol | CPSF-73, CPSF73 |
| Species | Human |
| Gene Type | protein-coding |
| Description | cleavage and polyadenylation specific factor 3, 73kDa |
| Other Description | cleavage and polyadenylation specificity factor subunit 3|mRNA 3'-end-processing endonuclease CPSF-73 |
| Swissprots | Q53RS2 Q96F36 O14769 Q9UKF6 |
| Accessions | AAY14858 EAX00989 EAX00990 Q9UKF6 AA635764 AF017269 AAB70268 AF171877 AAF00224 AK025440 AK223487 BAD97207 AK298918 BAG61024 BC011654 AAH11654 BC014106 BC020211 AAH20211 BC030988 AAH30988 BC043432 DC420903 EF036508 ABO65094 EU176402 ABW03853 XM_005246167 XP_005246224 XM_005246168 XP_005246225 XM_011510362 XP_011508664 NM_016207 NP_057291 |
| Function | Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as mRNA 3'-end-processing endonuclease. Also involved in the histone 3'-end pre-mRNA processing. U7 snRNP- dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete remov |
| Subcellular Location | Nucleus {ECO:0000269|PubMed:15037765}. |
| Top Pathways | mRNA surveillance pathway |
CPSF3 CRISPR/Cas9 KO Plasmid (h) - sc-407314 from Santa Cruz Biotechnology
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CPSF3 HDR Plasmid (h) - sc-407314-HDR from Santa Cruz Biotechnology
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CPSF3 Double Nickase Plasmid (h) - sc-407314-NIC from Santa Cruz Biotechnology
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CPSF3 Double Nickase Plasmid (h2) - sc-407314-NIC-2 from Santa Cruz Biotechnology
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CPSF3 CRISPR Activation Plasmid (h) - sc-407314-ACT from Santa Cruz Biotechnology
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CPSF3 CRISPR Activation Plasmid (h2) - sc-407314-ACT-2 from Santa Cruz Biotechnology
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CPSF3 Lentiviral Activation Particles (h) - sc-407314-LAC from Santa Cruz Biotechnology
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CPSF3 Lentiviral Activation Particles (h2) - sc-407314-LAC-2 from Santa Cruz Biotechnology
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CPSF3 siRNA (h) - sc-105243 from Santa Cruz Biotechnology
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CPSF3 shRNA Plasmid (h) - sc-105243-SH from Santa Cruz Biotechnology
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CPSF3 shRNA (h) Lentiviral Particles - sc-105243-V from Santa Cruz Biotechnology
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CPSF73 RNAi - H00051692-R01 from Novus Biologicals
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MISSION® esiRNA - EHU010081 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN6524 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND6524 from Sigma-Aldrich
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CPSF3 siRNA (Human) - CRH9902 from Cohesion Biosciences
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CPSF3 - MBS8227343 from MyBioSource
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shRNA set against Human CPSF3 (NM_016207.3) - SHH268933 from Creative biogene
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Human CPSF3 siRNA - orb270404 from Biorbyt
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CPSF3 shRNA Plasmid (Human) - CHH9902 from Cohesion Biosciences
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CPSF3 miRNA 3'UTR clone - MiUTR3H-08154 from Creative biogene
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