Gene Symbol | NDST3 |
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Entrez Gene | 9348 |
Alt Symbol | HSST3 |
Species | Human |
Gene Type | protein-coding |
Description | N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 |
Other Description | GlcNAc N-deacetylase/ N-sulfotransferase 3|N-HSST 3|N-heparan sulfate sulfotransferase 3|NDST-3|bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 3|glucosaminyl N-deacetylase/N-sulfotransferase 3|hNDST-3 |
Swissprots | Q4W5C1 B4DI67 Q9UP21 O95803 Q6UWC5 Q4W5D0 |
Accessions | AAY41001 AAY41056 EAX06313 O95803 AF074924 AAD15978 AF076605 AAD46061 AK295439 BAG58379 AY358852 AAQ89211 BC034037 BC109309 AAI09310 BC109310 AAI09311 BE348441 HQ234312 XM_006714416 XP_006714479 XM_011532414 XP_011530716 XM_011532415 XP_011530717 XM_011532416 XP_011530718 NM_004784 NP_004775 |
Function | Essential bifunctional enzyme that catalyzes both the N- deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has high deacetylase activity but low sulfotransferase activity. {ECO:0000269|PubMed:9915799}. |
Subcellular Location | Golgi apparatus membrane {ECO:0000250}; Single-pass type II membrane protein {ECO:0000250}. |
Tissue Specificity | Expressed in brain, kidney, liver, fetal and adult lung, adult pancreas, placenta, fetal spleen and fetal thymus. Not detected in adult/ fetal heart and skeletal muscle. {ECO:0000269|PubMed:9915799}. |
Top Pathways | Glycosaminoglycan biosynthesis - heparan sulfate / heparin |
NDST3 CRISPR/Cas9 KO Plasmid (h) - sc-411272 from Santa Cruz Biotechnology
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NDST3 HDR Plasmid (h) - sc-411272-HDR from Santa Cruz Biotechnology
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NDST3 Double Nickase Plasmid (h) - sc-411272-NIC from Santa Cruz Biotechnology
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NDST3 Double Nickase Plasmid (h2) - sc-411272-NIC-2 from Santa Cruz Biotechnology
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NDST3 CRISPR Activation Plasmid (h) - sc-411272-ACT from Santa Cruz Biotechnology
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NDST3 CRISPR Activation Plasmid (h2) - sc-411272-ACT-2 from Santa Cruz Biotechnology
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NDST3 Lentiviral Activation Particles (h) - sc-411272-LAC from Santa Cruz Biotechnology
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NDST3 Lentiviral Activation Particles (h2) - sc-411272-LAC-2 from Santa Cruz Biotechnology
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NDST3 siRNA (h) - sc-40765 from Santa Cruz Biotechnology
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NDST3 shRNA Plasmid (h) - sc-40765-SH from Santa Cruz Biotechnology
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NDST3 shRNA (h) Lentiviral Particles - sc-40765-V from Santa Cruz Biotechnology
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NDST3 RNAi - H00009348-R01 from Novus Biologicals
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MISSION® esiRNA - EHU057001 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN15132 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND15132 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR00247 from Sigma-Aldrich
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NDST3 siRNA (Human) - CRH6186 from Cohesion Biosciences
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Human NDST3 siRNA - orb266688 from Biorbyt
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NDST3 shRNA Plasmid (Human) - CHH6186 from Cohesion Biosciences
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NDST3 miRNA 3'UTR clone - MiUTR4H-TG05907 from Creative biogene
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