Gene Symbol | PPP4R2 |
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Entrez Gene | 151987 |
Alt Symbol | PP4R2 |
Species | Human |
Gene Type | protein-coding |
Description | protein phosphatase 4, regulatory subunit 2 |
Other Description | serine/threonine-protein phosphatase 4 regulatory subunit 2 |
Swissprots | A8K1I6 Q8WXX6 Q498B8 Q9NY27 Q2TAJ9 |
Accessions | EAW65530 Q9NY27 AF327345 AAL56006 AJ271448 CAB93534 AK289901 BAF82590 AL833216 BC100281 AAI00282 BC110889 AAI10890 BC128136 AAI28137 BC128137 AAI28138 NM_174907 NP_777567 |
Function | Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Mediates RPA2 dephosphorylation by recruiting PPP4C to RPA2 in a DNA damage-dependent manner. RPA2 dephosphorylation is required for the efficient RPA2-mediated recruitment of RAD51 to chromatin following double strand breaks, an essential step for DNA repair. {ECO:0000269|PubMed:10769191, ECO:0000269|PubMed:12668731, ECO:0000269|PubMed:18614045, ECO:0000269|PubMed:20154705}. |
Subcellular Location | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Note=Ionizing radiation induces relocalization to nuclear foci and colocalization with RPA2. |
Tissue Specificity | Widely expressed. {ECO:0000269|PubMed:12668731}. |
PP4R2 CRISPR/Cas9 KO Plasmid (h) - sc-407439 from Santa Cruz Biotechnology
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PP4R2 HDR Plasmid (h) - sc-407439-HDR from Santa Cruz Biotechnology
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PP4R2 Double Nickase Plasmid (h) - sc-407439-NIC from Santa Cruz Biotechnology
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PP4R2 Double Nickase Plasmid (h2) - sc-407439-NIC-2 from Santa Cruz Biotechnology
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PP4R2 CRISPR Activation Plasmid (h) - sc-407439-ACT from Santa Cruz Biotechnology
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PP4R2 CRISPR Activation Plasmid (h2) - sc-407439-ACT-2 from Santa Cruz Biotechnology
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PP4R2 Lentiviral Activation Particles (h) - sc-407439-LAC from Santa Cruz Biotechnology
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PP4R2 Lentiviral Activation Particles (h2) - sc-407439-LAC-2 from Santa Cruz Biotechnology
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PP4R2 siRNA (h) - sc-78526 from Santa Cruz Biotechnology
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PP4R2 shRNA Plasmid (h) - sc-78526-SH from Santa Cruz Biotechnology
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PP4R2 shRNA (h) Lentiviral Particles - sc-78526-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU133151 from Sigma-Aldrich
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MISSION® Human Phosphatase - SIHP0673 from Sigma-Aldrich
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MISSION® Human Phosphatase - SIHP0675 from Sigma-Aldrich
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MISSION® Human Phosphatase - SIHP0674 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN17312 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND17312 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR00206 from Sigma-Aldrich
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PPP4R2 siRNA (Human) - CRJ5720 from Cohesion Biosciences
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shRNA set against Human PPP4R2(NM_174907.2) - SHR135732 from Creative biogene
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Human PPP4R2 siRNA - orb276221 from Biorbyt
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PPP4R2 shRNA Plasmid (Human) - CHJ5720 from Cohesion Biosciences
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PPP4R2 miRNA 3'UTR clone - MiUTR1H-08199 from Creative biogene
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