Gene Symbol | NIT2 |
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Entrez Gene | 56954 |
Alt Symbol | HEL-S-8a |
Species | Human |
Gene Type | protein-coding |
Description | nitrilase family, member 2 |
Other Description | Nit protein 2|epididymis secretory sperm binding protein Li 8a|nitrilase homolog 2|omega-amidase NIT2 |
Swissprots | Q9NQR4 B2R9A3 Q8WUF0 D3DN47 |
Accessions | EAW79824 EAW79825 Q9NQR4 AF260334 AAG44665 AF284574 AAF87103 AK225979 AK294010 BAG57372 AK295835 BAH12195 AK313704 BAG36450 BC020620 AAH20620 BC107890 AAI07891 BM748852 DB466042 DQ893135 ABM84061 DQ896416 ABM87415 FJ224326 ACI46018 NM_020202 NP_064587 |
Function | Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha- ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle. {ECO:0000269|PubMed:17488281, ECO:0000269|PubMed:19595734}. |
Subcellular Location | Cytoplasm {ECO:0000269|PubMed:17488281}. |
Tissue Specificity | Detected in fetal brain (at protein level). Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, prostate, spleen, thymus, prostate, testis, ovary, small intestine and colon. {ECO:0000269|PubMed:14528910, ECO:0000269|PubMed:17488281}. |
Top Pathways | Alanine, aspartate and glutamate metabolism |
NIT2 CRISPR/Cas9 KO Plasmid (h) - sc-412737 from Santa Cruz Biotechnology
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NIT2 HDR Plasmid (h) - sc-412737-HDR from Santa Cruz Biotechnology
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NIT2 Double Nickase Plasmid (h) - sc-412737-NIC from Santa Cruz Biotechnology
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NIT2 Double Nickase Plasmid (h2) - sc-412737-NIC-2 from Santa Cruz Biotechnology
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NIT2 CRISPR Activation Plasmid (h) - sc-412737-ACT from Santa Cruz Biotechnology
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NIT2 CRISPR Activation Plasmid (h2) - sc-412737-ACT-2 from Santa Cruz Biotechnology
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NIT2 Lentiviral Activation Particles (h) - sc-412737-LAC from Santa Cruz Biotechnology
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NIT2 Lentiviral Activation Particles (h2) - sc-412737-LAC-2 from Santa Cruz Biotechnology
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NIT2 siRNA (h) - sc-78047 from Santa Cruz Biotechnology
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NIT2 shRNA Plasmid (h) - sc-78047-SH from Santa Cruz Biotechnology
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NIT2 shRNA (h) Lentiviral Particles - sc-78047-V from Santa Cruz Biotechnology
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CompoZr® Knockout ZFN Kit - CKOZFN15312 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND15312 from Sigma-Aldrich
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NIT2 siRNA (Human) - CRJ1253 from Cohesion Biosciences
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shRNA set against Human NIT2 (NM_020202.4) - SHH352608 from Creative biogene
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Human NIT2 siRNA - orb271754 from Biorbyt
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NIT2 shRNA Plasmid (Human) - CHJ1253 from Cohesion Biosciences
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NIT2 miRNA 3'UTR clone - MiUTR3H-15588 from Creative biogene
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