| Gene Symbol | PHF8 |
|---|---|
| Entrez Gene | 23133 |
| Alt Symbol | JHDM1F, MRXSSD, ZNF422 |
| Species | Human |
| Gene Type | protein-coding |
| Description | PHD finger protein 8 |
| Other Description | histone lysine demethylase PHF8|jumonji C domain-containing histone demethylase 1F |
| Swissprots | Q7Z6D4 Q9HAH2 Q5JPS3 Q5JPS0 Q5JPR9 Q5VUJ4 Q5JPS2 Q5H9U5 Q9UPP1 B3KMV4 B7Z911 |
| Accessions | EAW93167 EAW93168 EAW93169 EAW93170 Q9UPP1 AB029034 BAA83063 AF091081 AAC72950 AK021696 BAB13877 AK022788 BAG51116 AK027229 AK304272 BAH14147 BC017720 BC042108 BC053861 AAH53861 BE676640 CR933612 CAI45929 DA458639 DB079048 XM_005261996 XP_005262053 XM_005261997 XP_005262054 XM_005261999 XP_005262056 XM_005262000 XP_005262057 XM_006724585 XP_006724648 XM_011530778 XP_011529080 NM_001184896 NP_001171825 NM_001184897 NP_001171826 NM_001184898 NP_001171827 NM_015107 NP_055922 |
| Function | Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in |
| Subcellular Location | Nucleus. Nucleus, nucleolus. Note=Recruited to H3K4me3 sites on chromatin during interphase. Dissociates from chromatin when cells enter mitosis. |
PHF8 CRISPR/Cas9 KO Plasmid (h) - sc-407306 from Santa Cruz Biotechnology
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PHF8 HDR Plasmid (h) - sc-407306-HDR from Santa Cruz Biotechnology
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PHF8 Double Nickase Plasmid (h) - sc-407306-NIC from Santa Cruz Biotechnology
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PHF8 Double Nickase Plasmid (h2) - sc-407306-NIC-2 from Santa Cruz Biotechnology
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PHF8 CRISPR Activation Plasmid (h) - sc-407306-ACT from Santa Cruz Biotechnology
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PHF8 CRISPR Activation Plasmid (h2) - sc-407306-ACT-2 from Santa Cruz Biotechnology
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PHF8 Lentiviral Activation Particles (h) - sc-407306-LAC from Santa Cruz Biotechnology
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PHF8 Lentiviral Activation Particles (h2) - sc-407306-LAC-2 from Santa Cruz Biotechnology
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PHF8 siRNA (h) - sc-91011 from Santa Cruz Biotechnology
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PHF8 shRNA Plasmid (h) - sc-91011-SH from Santa Cruz Biotechnology
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PHF8 shRNA (h) Lentiviral Particles - sc-91011-V from Santa Cruz Biotechnology
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PHF8 RNAi - H00023133-R01 from Novus Biologicals
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MISSION® esiRNA - EHU062731 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN16735 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND16735 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR12932 from Sigma-Aldrich
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PHF8 siRNA (Human) - CRH8022 from Cohesion Biosciences
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PHF8 CRISPR/Cas9 KO Plasmid (h2) - sc-407306-KO-2 from Santa Cruz Biotechnology
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PHF8 HDR Plasmid (h2) - sc-407306-HDR-2 from Santa Cruz Biotechnology
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shRNA set against Human PHF8 (NM_015107.2) - SHH374832 from Creative biogene
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Human PHF8 siRNA - orb268524 from Biorbyt
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PHF8 shRNA Plasmid (Human) - CHH8022 from Cohesion Biosciences
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PHF8 miRNA 3'UTR clone - MiUTR4H-TG06645 from Creative biogene
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