HUS1

Gene Symbol HUS1
Entrez Gene 3364
Alt Symbol hHUS1
Species Human
Gene Type protein-coding
Description HUS1 checkpoint homolog (S. pombe)
Other Description checkpoint protein HUS1|hus1+-like protein
Swissprots B4DFI9 O60921
Accessions AAM18968 EAL23807 EAW61001 EAW61002 O60921 AF076844 AAC95526 AF110393 AAD25350 AJ227901 AK290459 BAF83148 AK294117 BAG57450 BC007013 AAH07013 BG286955 BT019481 AAV38288 BT019482 AAV38289 CA438762 CR536552 CAG38789 Y16893 CAA76518 NM_004507 NP_004498 NR_037917
Function Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. {ECO:0000269|PubMed:21659603}.
Subcellular Location Nucleus. Cytoplasm. Note=In discrete nuclear foci upon DNA damage. According to PubMed:14500360, localized also in the cytoplasm. DNA damage induces its nuclear translocation. Shuttles between the nucleus and the cytoplasm.
Tissue Specificity Ubiquitous. {ECO:0000269|PubMed:10777662}.

Hus1 (E-21) - sc-30543 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Hus1 (E-21): sc-30543. Western blot analysis of Hus1 expression in K-562 (A), HeLa (B), PMA-treated A-431 (C) and UV-treated HeLa (D) whole cell lysates and HeLa nuclear extract (E).

Hus1 (M-281) - sc-8323 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Hus1 (M-281): sc-8323. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic and nuclear staining of cells in seminiferous ducts and Leydig cells.

Hus1 (G-3) - sc-166440 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Hus1 (G-3): sc-166440. Western blot analysis of Hus1 expression in NIH/3T3 nuclear extract (A) and NIH/3T3 whole cell lysate (B).

Hus1 (S-21) - sc-30542 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Hus1 (S-21): sc-30542. Western blot analysis of Hus1 expression in K-562 (A), HeLa (B), HEK293 (C) and Jurkat (D) whole cell lysates.

Anti-HUS1 antibody - ab186850 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186850. Blue DAPI for nuclear staining.

Anti-HUS1 antibody - ab130520 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-HUS1 antibody (ab130520) at 1/100 dilution + NCI-H460 cell line lysate at 35 µg

Anti-HUS1 antibody [EPR5132] - ab109371 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5132
Overlay histogram showing HeLa cells stained with ab109371 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109371, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-HUS1 Antibody (aa199-226, AP) - LS-C215198 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HUS1 Antibody (aa199-226, APC) - LS-C215199 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HUS1 Antibody (aa199-226, Biotin) - LS-C215200 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HUS1 Antibody (aa199-226, FITC) - LS-C215201 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HUS1 Antibody (aa199-226, Azide-free, HRP) - LS-C215202 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HUS1 Antibody (aa199-226, PE) - LS-C215204 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HUS1 Antibody - LS-C308354 from LifeSpan Bioscience

Prices $455.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-HUS1 Antibody - LS-C310672 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HUS1 Antibody (Biotin) - LS-C317651 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-HUS1 Antibody - LS-C310673 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-HUS1 Antibody (FITC) - LS-C317652 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-HUS1 Antibody (aa199-226) - LS-C165090 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
HUS1 Antibody western blot of NCI-H460 cell line lysates (35 ug/lane). The HUS1 antibody detected the HUS1 protein (arrow).

Anti-HUS1 Antibody (clone 2B12) - LS-C197476 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2B12
Immunoprecipitation of HUS1 transfected lysate using anti-HUS1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HUS1 rabbit polyclonal antibody.

Anti-HUS1 Antibody (HRP) - LS-C317663 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-HUS1 Antibody (aa198-226) - LS-C322127 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-HUS1 Antibody - LS-C341608 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-HUS1 Antibody (Internal, clone EPR5132) - LS-C138290 from LifeSpan Bioscience

Prices $450.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5132
IHC of paraffin-embedded human breast carcinoma tissue using anti-HUS1 antibody.

Anti-HUS1 Antibody (aa1-273) - LS-C186327 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded CA922 xenograft using HUS1 antibody at 1:500 dilution.
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