SMYD2

Gene Symbol SMYD2
Entrez Gene 56950
Alt Symbol HSKM-B, KMT3C, ZMYND14
Species Human
Gene Type protein-coding
Description SET and MYND domain containing 2
Other Description N-lysine methyltransferase SMYD2|SET and MYND domain-containing protein 2|histone methyltransferase SMYD2|lysine N-methyltransferase 3C|zinc finger, MYND domain containing 14
Swissprots I6L9H7 Q9NRG4 Q96AI4 Q5VSH9 Q4V765 B2R9P9
Accessions EAW93355 EAW93356 EAW93357 EAW93358 Q9NRG4 AF070592 AF226053 AAF86953 AK091590 AK098683 AK313868 BAG36596 AW474469 BC017080 AAH17080 BC028104 AAH28104 BC039890 BC049367 AAH49367 BC057793 BC068511 BC094747 BC098133 AAH98133 BC098276 AAH98276 BC098305 AAH98305 BC098335 AAH98335 BG723585 XR_426790 NM_020197 NP_064582
Function Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins, including p53/TP53 and RB1. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Shows even higher methyltransferase activity on p53/TP53. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates RB1 at 'Lys-860'. {ECO:0000269|PubMed:17108971, ECO:0000269|PubMed:17805299, ECO:0000269|PubMed:18065756, ECO:0000269|PubMed:20870719, ECO:0000269|PubMed:21782458, ECO:0000269|PubMed:21880715}.
Subcellular Location Cytoplasm, cytosol {ECO:0000250}. Nucleus {ECO:0000250}.

SMYD2 (D14H7) Rabbit mAb - 9734 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D14H7
Western blot analysis of extracts from various cell lines using SMYD2 (D14H7) Rabbit mAb.

SMYD2 Antibody - 4251 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using SMYD2 Antibody.

SMYD2 (F-9) - sc-393827 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SMYD2 (F-9): sc-393827. Western blot analysis of SMYD2 expression in human heart tissue extract (A) and Hep G2 (B), HeLa (C), Jurkat (D) and RT-4 (E) whole cell lysates.

SMYD2 (H-252) - sc-367580 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SMYD2 (H-252): sc-367580. Western blot analysis of SMYD2 expression in non-transfected: sc-117750 (A) and mouse SMYD2 transfected: sc-123669 (B) whole cell lysates.

SMYD2 (N-14) - sc-79086 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SMYD2 (N-14): sc-79086. Western blot analysis of SMYD2 expression in non-transfected: sc-117752 (A) and mouse SMYD2 transfected: sc-123669 (B) 293T whole cell lysates.

SMYD2 (C-20) - sc-79084 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SMYD2 (C-20): sc-79084. Western blot analysis of SMYD2 expression in non-transfected: sc-117752 (A) and mouse SMYD2 transfected: sc-123669 (B) 293T whole cell lysates.

SMYD2 (Y-22) - sc-130879 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
SMYD2 (Y-22): sc-130879. Western blot analysis of SMYD2 expression in non-transfected (A) and human SMYD2 transfected (B) 293 whole cell lysates.

Anti-KMT3C / SMYD2 antibody - ab135849 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-KMT3C / SMYD2 antibody (ab135849) at 1/500 dilution + HT29 cell extracts at 30 µg

Anti-KMT3C / SMYD2 antibody [1G10-E11-H9] - ab151183 from Abcam

Host Mouse
Clonality Monoclonal
Clone 1G10-E11-H9
All lanes : Anti-KMT3C / SMYD2 antibody [1G10-E11-H9] (ab151183) at 1/1000 dilutionLane 1 : 3T3 cell lysateLane 2 : HT1080 cell lysateLane 3 : A431 cell lysateLane 4 : Jurkat cell lysate

Anti-KMT3C / SMYD2 antibody - N-terminal - ab71235 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-KMT3C / SMYD2 antibody - N-terminal (ab71235) at 1/50 dilutionLane 1 : 293 cell lysates (non transfected)Lane 2 : 293 cell lysates transiently transfected with the KMT3C / SMYD2 geneLysates/proteins at 2 µg per lane.

Anti-SMYD2 Antibody (Leu148) - LS-C178248 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of SMYD2 (L148) pAb in extracts from HT29 cells.

Anti-SMYD2 Antibody (aa383-433) - LS-C289716 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human and Mouse SMYD2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SMYD2 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SMYD2 was also immunoprecipitated by rabbit anti-SMYD2 antibody BL10995, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Anti-SMYD2 Antibody (aa107-156) - LS-C108014 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SMYD2 antibody LS-C108014 Western blot of ACHN lysate.

Anti-SMYD2 Antibody (C-Terminus) - LS-C111286 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SMYD2 antibody LS-C111286 Western blot of SH-SYSY lysate.

Anti-SMYD2 Antibody (aa99-148) - LS-C200005 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of SMYD2 antibody in extracts from HT29 cells.

Anti-SMYD2 Antibody - LS-C334744 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using SMYD2 antibody.

Anti-SMYD2 Antibody (aa120-152) - LS-C96587 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
SMYD2 Antibody western blot of mouse Neuro-2a cell line lysates (35 ug/lane). The SMYD2 antibody detected the SMYD2 protein (arrow).

Anti-SMYD2 Antibody - LS-C332065 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-SMYD2 Antibody - LS-C342035 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

SMYD2 Antibody - NBP2-20429 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: SMYD2 Antibody [NBP2-20429] - Sample (30 ug of whole cell lysate) A: Hela B: HCT116 10% SDS PAGE gel, diluted at 1:1000.

SMYD2 Antibody - NBP1-50043 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SMYD2 Antibody - NBP2-13034 from Novus Biologicals

Prices $349.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: SMYD2 Antibody [NBP2-13034] - Extracts from HT29 cells.

SMYD2 Antibody - NBP1-83186 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

SMYD2 Antibody - H00056950-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: SMYD2 Antibody [H00056950-B01P] - Analysis of SMYD2 expression in transfected 293T cell line by SMYD2 polyclonal antibody. Lane 1: SMYD2 transfected lysate(47.63 KDa). Lane 2: Non-transfected lysate.

SMYD2 Antibody - H00056950-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: SMYD2 Antibody [H00056950-D01P] - Analysis of SMYD2 expression in transfected 293T cell line by SMYD2 polyclonal antibody.Lane 1: SMYD2 transfected lysate(49.70 KDa).Lane 2: Non-transfected lysate.
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