Gene Symbol | SRD5A3 |
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Entrez Gene | 79644 |
Alt Symbol | CDG1P, CDG1Q, KRIZI, SRD5A2L, SRD5A2L1 |
Species | Human |
Gene Type | protein-coding |
Description | steroid 5 alpha-reductase 3 |
Other Description | 3-oxo-5-alpha-steroid 4-dehydrogenase 3|S5AR 3|SR type 3|polyprenol reductase|probable polyprenol reductase |
Swissprots | Q4W5Q6 Q9H8P0 |
Accessions | AAY40904 EAX05465 Q9H8P0 AK023414 BAB14568 BC002480 AAH02480 CR457312 CAG33593 CX763068 DB532427 HQ447832 ADQ32316 XM_005265766 XP_005265823 XM_005265767 XP_005265824 NM_024592 NP_078868 |
Function | Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. Also able to convert testosterone (T) into 5-alpha-dihydrotestosterone (DHT). {ECO:0000269|PubMed:17986282, ECO:0000269|PubMed:20637498}. |
Subcellular Location | Endoplasmic reticulum membrane {ECO:0000305|PubMed:20637498}; Multi-pass membrane protein {ECO:0000305|PubMed:20637498}. |
Tissue Specificity | Overexpressed in hormone-refractory prostate cancers (HRPC). Almost no or little expression in normal adult organs. {ECO:0000269|PubMed:17986282}. |
Top Pathways | Steroid hormone biosynthesis |
SRD5A2L CRISPR/Cas9 KO Plasmid (h) - sc-413197 from Santa Cruz Biotechnology
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SRD5A2L HDR Plasmid (h) - sc-413197-HDR from Santa Cruz Biotechnology
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SRD5A2L Double Nickase Plasmid (h) - sc-413197-NIC from Santa Cruz Biotechnology
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SRD5A2L Double Nickase Plasmid (h2) - sc-413197-NIC-2 from Santa Cruz Biotechnology
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SRD5A2L CRISPR Activation Plasmid (h) - sc-413197-ACT from Santa Cruz Biotechnology
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SRD5A2L CRISPR Activation Plasmid (h2) - sc-413197-ACT-2 from Santa Cruz Biotechnology
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SRD5A2L Lentiviral Activation Particles (h) - sc-413197-LAC from Santa Cruz Biotechnology
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SRD5A2L Lentiviral Activation Particles (h2) - sc-413197-LAC-2 from Santa Cruz Biotechnology
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SRD5A2L siRNA (h) - sc-89215 from Santa Cruz Biotechnology
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SRD5A2L shRNA Plasmid (h) - sc-89215-SH from Santa Cruz Biotechnology
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SRD5A2L shRNA (h) Lentiviral Particles - sc-89215-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU111731 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN20562 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND20562 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR08577 from Sigma-Aldrich
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SRD5A3 siRNA (Human) - CRJ2451 from Cohesion Biosciences
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SRD5A3 - MBS8239496 from MyBioSource
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shRNA set against Human SRD5A3 (NM_024592.4) - SHH417716 from Creative biogene
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Human SRD5A3 siRNA - orb272952 from Biorbyt
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SRD5A3 shRNA Plasmid (Human) - CHJ2451 from Cohesion Biosciences
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SRD5A3 miRNA 3'UTR clone - MiUTR3H-12060 from Creative biogene
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