DDIT4

Gene Symbol DDIT4
Entrez Gene 54541
Alt Symbol Dig2, REDD-1, REDD1
Species Human
Gene Type protein-coding
Description DNA-damage-inducible transcript 4
Other Description DNA damage-inducible transcript 4 protein|HIF-1 responsive protein RTP801|protein regulated in development and DNA damage response 1
Swissprots Q9H0S3 Q9NX09
Accessions EAW54452 EAW54453 Q9NX09 AF335324 AAL38424 AK000507 BAA91214 AL136668 CAB66603 AM392607 CAL37485 AY090097 AAM10442 BC000708 AAH00708 BC007714 AAH07714 BC015236 AAH15236 BE882343 BM843387 CN428398 DQ890984 ABM81910 DQ894159 ABM85085 NM_019058 NP_061931
Function Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes (By similarity). Required for mTORC1-mediated defense against viral protein synthesis and virus replication (By similarity). Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death. {ECO:0000250, ECO:0000
Subcellular Location Mitochondrion {ECO:0000250}. Cytoplasm, cytosol {ECO:0000269|PubMed:12453409}.
Tissue Specificity Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients (at protein level). {ECO:0000269|PubMed:11884613, ECO:0000269|PubMed:12453409, ECO:0000269|PubMed:17005863, ECO:0000269|PubMed:17379067}.
Top Pathways PI3K-Akt signaling pathway, MicroRNAs in cancer, mTOR signaling pathway

REDD1 Antibody - 2516 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from COS-7 cells, transfected with HA-REDD1 or untransfected (mock), using REDD1 Antibody.

REDD-1 (A-4) - sc-271158 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
REDD-1 (A-4): sc-271158. Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells.

REDD-1 (H-110) - sc-67051 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
REDD-1 (H-110): sc-67051. Western blot analysis of REDD-1 expression in non-transfected: sc-110760 (A) and human REDD-1 transfected: sc-111360 (B) 293 whole cell lysates.

REDD-1 (B-3) - sc-376671 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
REDD-1 (B-3): sc-376671. Western blot analysis of REDD-1 expression in non-transfected: sc-110760 (A) and human REDD-1 transfected: sc-111360 (B) 293 whole cell lysates.

REDD-1 (K-14) - sc-46033 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

REDD-1 (N-20) - sc-46034 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
REDD-1 (N-20): sc-46034. Western blot analysis of REDD-1 expression in non-transfected: sc-110760 (A) and human REDD-1 transfected: sc-111360 (B) 293 whole cell lysates.

Anti-DDIT4 (phospho T23 + T25) antibody - ab183468 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DDIT4 (phospho T23 + T25) antibody (ab183468) at 1/500 dilutionLane 1 : Jurkat cell lysateLane 2 : Jurkat cell lysate with immunizing phosphopeptide

Anti-DDIT4 antibody - ab63059 from Abcam

Prices $390.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-DDIT4 antibody (ab63059) at 1/60 dilution + Jurkat cell line lysates at 35 µg

Anti-DDIT4 antibody - ab106356 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-DDIT4 antibody (ab106356) at 0.5 µg/mlLane 2 : Anti-DDIT4 antibody (ab106356) at 1 µg/mlLane 1 : Human kidney tissue lysateLane 2 : Human kidney tissue lysateLysates/proteins at 15 µg per lane.

Anti-DDIT4 antibody - ab124213 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-DDIT4 antibody (ab124213) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-DDIT4 antibody - ab99929 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunostaining of Human DDIT4 in NBF-fixed asynchronous HeLa cells. Cobalt treated cells (left) or mock treated cells (right). ab99929 used at a dilution of 1/100. Detection: Red fluorescent goat anti-rabbit IgG H&L cross-adsorbed Antibody DyLight®594.

Anti-DDIT4 antibody - ab172787 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-DDIT4 antibody (ab172787) at 1 µg/mlLane 1 : DDIT4 transfected 293T cell line lysateLane 2 : Non-transfected 293T cell line lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG at 1/2500 dilution

Anti-DDIT4 antibody - N-terminal - ab157543 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-DDIT4 antibody - N-terminal (ab157543) at 1 µg/ml + Rat thymus lysate at 10 µg

Anti-DDIT4 / REDD1 Antibody (aa1-50) - LS-C288925 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human REDD1 by Immunocytochemistry. Sample: NBF-fixed asynchronous, Cobalt-treated HeLa cells. Antibody: Affinity purified rabbit anti-REDD1 used at a dilution of 1:500 (2 ug/ml). Detection: Red-fluorescent goat anti-rabbit IgG H&L cross-adsorbed Antibody DyLight594(A120-601D4) used at a dilution of 1:100.

Anti-DDIT4 / REDD1 Antibody (aa1-50) - LS-C286755 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human REDD1 by Immunocytochemistry. Sample: NBF-fixed asynchronous HeLa cells. Cobalt treated cells (left) or mock treated cells (right) immunostained for REDD1. Antibody: Affinity purified rabbit anti-REDD1 used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG H&L cross-adsorbed Antibody DyLight594(A120-601D4) used at a dilution of 1:100.

Anti-DDIT4 / REDD1 Antibody (aa198-227) - LS-C167322 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
DDIT4 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human pancreas tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-DDIT4 / REDD1 Antibody (Asn60) - LS-C75353 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-DDIT4 / REDD1 Antibody (aa50-100) - LS-C148959 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-DDIT4 / REDD1 Antibody (aa54-71) - LS-C148985 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-DDIT4 / REDD1 Antibody (C-Terminus, AP) - LS-C255951 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DDIT4 / REDD1 Antibody (C-Terminus, APC) - LS-C255952 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DDIT4 / REDD1 Antibody (N-Terminus) - LS-C53272 from LifeSpan Bioscience

Prices $325.00, $890.00
Sizes 50 µg, 500 µg
Host Rabbit
Clonality Polyclonal
Western blot of RTP801 in 293 cell lysate with RTP801 antibody at (A) 2 and (B) 4 ug/ml.

Anti-DDIT4 / REDD1 Antibody (Internal) - LS-C53268 from LifeSpan Bioscience

Prices $345.00, $1,035.00
Sizes 50 µg, 500 µg
Host Rabbit
Clonality Polyclonal
Western blot of RTP801 in human kidney tissue lysate with RTP801 antibody at (A) 0.5 and (B) 1 ug/ml.

Anti-DDIT4 / REDD1 Antibody (C-Terminus, Biotin) - LS-C255953 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-DDIT4 / REDD1 Antibody (C-Terminus, FITC) - LS-C255956 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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