| Gene Symbol | DDIT4 |
|---|---|
| Entrez Gene | 54541 |
| Alt Symbol | Dig2, REDD-1, REDD1 |
| Species | Human |
| Gene Type | protein-coding |
| Description | DNA-damage-inducible transcript 4 |
| Other Description | DNA damage-inducible transcript 4 protein|HIF-1 responsive protein RTP801|protein regulated in development and DNA damage response 1 |
| Swissprots | Q9H0S3 Q9NX09 |
| Accessions | EAW54452 EAW54453 Q9NX09 AF335324 AAL38424 AK000507 BAA91214 AL136668 CAB66603 AM392607 CAL37485 AY090097 AAM10442 BC000708 AAH00708 BC007714 AAH07714 BC015236 AAH15236 BE882343 BM843387 CN428398 DQ890984 ABM81910 DQ894159 ABM85085 NM_019058 NP_061931 |
| Function | Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type; it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes (By similarity). Required for mTORC1-mediated defense against viral protein synthesis and virus replication (By similarity). Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death. {ECO:0000250, ECO:0000 |
| Subcellular Location | Mitochondrion {ECO:0000250}. Cytoplasm, cytosol {ECO:0000269|PubMed:12453409}. |
| Tissue Specificity | Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients (at protein level). {ECO:0000269|PubMed:11884613, ECO:0000269|PubMed:12453409, ECO:0000269|PubMed:17005863, ECO:0000269|PubMed:17379067}. |
| Top Pathways | PI3K-Akt signaling pathway, MicroRNAs in cancer, mTOR signaling pathway |
REDD-1 CRISPR/Cas9 KO Plasmid (h) - sc-401797 from Santa Cruz Biotechnology
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REDD-1 HDR Plasmid (h) - sc-401797-HDR from Santa Cruz Biotechnology
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REDD-1 Double Nickase Plasmid (h) - sc-401797-NIC from Santa Cruz Biotechnology
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REDD-1 Double Nickase Plasmid (h2) - sc-401797-NIC-2 from Santa Cruz Biotechnology
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REDD-1 CRISPR Activation Plasmid (h) - sc-401797-ACT from Santa Cruz Biotechnology
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REDD-1 CRISPR Activation Plasmid (h2) - sc-401797-ACT-2 from Santa Cruz Biotechnology
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REDD-1 Lentiviral Activation Particles (h) - sc-401797-LAC from Santa Cruz Biotechnology
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REDD-1 Lentiviral Activation Particles (h2) - sc-401797-LAC-2 from Santa Cruz Biotechnology
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REDD-1 siRNA (h) - sc-45806 from Santa Cruz Biotechnology
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REDD-1 shRNA Plasmid (h) - sc-45806-SH from Santa Cruz Biotechnology
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REDD-1 shRNA (h) Lentiviral Particles - sc-45806-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU009051 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN7026 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND7026 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR06567 from Sigma-Aldrich
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DDIT4 siRNA (Human) - CRJ0124 from Cohesion Biosciences
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DDIT4 - MBS8219530 from MyBioSource
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REDD-1 CRISPR/Cas9 KO Plasmid (h2) - sc-401797-KO-2 from Santa Cruz Biotechnology
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REDD-1 HDR Plasmid (h2) - sc-401797-HDR-2 from Santa Cruz Biotechnology
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shRNA set against Human DDIT4 (NM_019058.2) - SHH275665 from Creative biogene
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shRNA set against Human DDIT4(NM_019058.2) - SHG226009 from Creative biogene
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DDIT4 shRNA Plasmid (Human) - CHJ0124 from Cohesion Biosciences
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DDIT4 miRNA 3'UTR clone - MiUTR3H-09426 from Creative biogene
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