NEIL1

Gene Symbol NEIL1
Entrez Gene 79661
Alt Symbol FPG1, NEI1, hFPG1
Species Human
Gene Type protein-coding
Description nei endonuclease VIII-like 1 (E. coli)
Other Description DNA glycosylase/AP lyase Neil1|DNA-(apurinic or apyrimidinic site) lyase Neil1|NEH1|endonuclease 8-like 1|endonuclease VIII|endonuclease VIII-like 1|nei homolog 1|nei-like protein 1
Swissprots Q96FI4 Q6ZRA7 Q86XW7 Q9H6C3 D3DW75
Accessions AAO74826 EAW99255 EAW99256 EAW99257 EAW99258 EAW99259 EAW99260 Q96FI4 AB079068 BAC06476 AK026055 BAB15337 AK026216 AK097008 AK128372 BC010876 AAH10876 DB282730 DQ891430 ABM82356 DQ894605 ABM85531 XM_005254659 XP_005254716 XM_006720677 XP_006720740 XM_006720678 XP_006720741 XM_006720679 XP_006720742 XM_006720680 XP_006720743 XM_006720681 XP_006720744 XM_011522001 XP_011520303 XM_011522002 XP_011520304 XM_011522003 XP_011520305 XM_011522004 XP_011520306 XM_011522005 XP_011520307 XM_011522006 XP_011520308 XM_011522007 XP_011520309 XM_011522008 XP_011520310 XR_243127 XR_429472 NM_001256552 NP_001243481 NM_024608 NP_078884 NR_046311
Function Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8- oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5- hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC. {ECO:0000269|PubMed:11904416, ECO:0000269|PubMed:12200441, ECO:0000269|PubMed:12509226, ECO:0000269|PubMed:14522990}.
Subcellular Location Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:17556049}. Nucleus {ECO:0000269|PubMed:17556049}. Chromosome {ECO:0000269|PubMed:17556049}. Note=During mitosis, associates with centrosomes and condensed chromatin.
Tissue Specificity Ubiquitous. {ECO:0000269|PubMed:11904416}.
Top Pathways Base excision repair

NEIL1 (B-1) - sc-271167 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NEIL1 (B-1): sc-271167. Western blot analysis of NEIL1 expression in non-transfected: sc-110760 (A) and human NEIL1 transfected: sc-111234 (B) 293 whole cell lysates.

NEIL1 (D-4) - sc-365302 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NEIL1 (D-4): sc-365302. Western blot analysis of NEIL1 expression in non-transfected: sc-110760 (A) and human NEIL1 transfected: sc-111234 (B) 293 whole cell lysates.

NEIL1 (D-18) - sc-47608 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

NEIL1 (D-1) - sc-271164 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
NEIL1 (D-1): sc-271164. Western blot analysis of NEIL1 expression in non-transfected: sc-117752 (A) and human NEIL1 transfected: sc-171612 (B) 293T whole cell lysates.

NEIL1 (H-49) - sc-134547 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
NEIL1 (H-49): sc-134547. Western blot analysis of NEIL1 expression in non-transfected: sc-110760 (A) and human NEIL1 transfected: sc-111234 (B) 293 whole cell lysates.

NEIL1 (S-17) - sc-47611 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
NEIL1 (S-17): sc-47611. Western blot analysis of NEIL1 expression in non-transfected 293: sc-110760 (A), human NEIL1 transfected 293: sc-111234 (B) and HeLa (C) whole cell lysates.

Anti-NEIL1 antibody - ab21337 from Abcam

Prices $392.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-NEIL1 antibody (ab21337) at 1/1000 dilution + Mouse ES whole cell lysate at 100 µgSecondaryHRP Goat anti-rabbit IgG polyclonal at 1/2000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-NEIL1 antibody - ab14753 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
ab14753 at 5ug/ml staining NEIL1 in human placental tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). A AP-staining procedure was used for detection.

Anti-NEIL1 antibody - ab192517 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-NEIL1 antibody - ab103137 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-NEIL1 antibody (ab103137) at 1/50 dilution + A2058 cell lysate at 35 µg

Anti-NEIL1 antibody - ab128294 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-NEIL1 antibody (ab128294) at 1 µg/ml + PANC-1 cell lysate at 10 µg

Anti-NEIL1 antibody - ab169598 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-NEIL1 antibody (ab169598) at 1 µg/mlLane 1 : NEIL1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique

Anti-NEIL1 antibody [1D1] - ab156786 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1D1
All lanes : Anti-NEIL1 antibody [1D1] (ab156786) at 1/200 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cells transfected with pCMV6-ENTRY NEIL1 cDNALysates/proteins at 5 µg per lane.

Anti-NEIL1 Antibody (aa130-159) - LS-C164822 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of NEIL1 Antibody in A2058 cell line lysates (35 ug/lane). NEIL1 (arrow) was detected using the purified antibody.

Anti-NEIL1 Antibody (aa291-340) - LS-C119957 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts from HeLa cells, using NEIL1 Antibody. The lane on the right is treated with the synthesized peptide.

Anti-NEIL1 Antibody - LS-C334322 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of A375 cell line, using NEIL1 antibody.

Anti-NEIL1 Antibody (aa186-390, clone 1D1) - LS-C336954 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1D1
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEIL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEIL1.

Anti-NEIL1 Antibody (aa282-331) - LS-C200265 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of the lysates from HeLa cells using NEIL1 antibody.

Anti-NEIL1 Antibody (aa378-390) IHC-plus™ - LS-B2278 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-NEIL1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2278 concentration 5 ug/ml.

Anti-NEIL1 Antibody - LS-C73214 from LifeSpan Bioscience

Prices $485.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-NEIL1 Antibody - LS-C318411 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-NEIL1 Antibody (FITC) - LS-C318413 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-NEIL1 Antibody (Biotin) - LS-C318414 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-NEIL1 Antibody (HRP) - LS-C318416 from LifeSpan Bioscience

Prices $305.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-NEIL1 Antibody (aa270-319) - LS-C151557 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
NEIL1 antibody Western Blot of PANC1.
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