NEIL1
| Gene Symbol | NEIL1 |
|---|---|
| Entrez Gene | 79661 |
| Alt Symbol | FPG1, NEI1, hFPG1 |
| Species | Human |
| Gene Type | protein-coding |
| Description | nei endonuclease VIII-like 1 (E. coli) |
| Other Description | DNA glycosylase/AP lyase Neil1|DNA-(apurinic or apyrimidinic site) lyase Neil1|NEH1|endonuclease 8-like 1|endonuclease VIII|endonuclease VIII-like 1|nei homolog 1|nei-like protein 1 |
| Swissprots | Q96FI4 Q6ZRA7 Q86XW7 Q9H6C3 D3DW75 |
| Accessions | AAO74826 EAW99255 EAW99256 EAW99257 EAW99258 EAW99259 EAW99260 Q96FI4 AB079068 BAC06476 AK026055 BAB15337 AK026216 AK097008 AK128372 BC010876 AAH10876 DB282730 DQ891430 ABM82356 DQ894605 ABM85531 XM_005254659 XP_005254716 XM_006720677 XP_006720740 XM_006720678 XP_006720741 XM_006720679 XP_006720742 XM_006720680 XP_006720743 XM_006720681 XP_006720744 XM_011522001 XP_011520303 XM_011522002 XP_011520304 XM_011522003 XP_011520305 XM_011522004 XP_011520306 XM_011522005 XP_011520307 XM_011522006 XP_011520308 XM_011522007 XP_011520309 XM_011522008 XP_011520310 XR_243127 XR_429472 NM_001256552 NP_001243481 NM_024608 NP_078884 NR_046311 |
| Function | Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8- oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5- hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC. {ECO:0000269|PubMed:11904416, ECO:0000269|PubMed:12200441, ECO:0000269|PubMed:12509226, ECO:0000269|PubMed:14522990}. |
| Subcellular Location | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:17556049}. Nucleus {ECO:0000269|PubMed:17556049}. Chromosome {ECO:0000269|PubMed:17556049}. Note=During mitosis, associates with centrosomes and condensed chromatin. |
| Tissue Specificity | Ubiquitous. {ECO:0000269|PubMed:11904416}. |
| Top Pathways | Base excision repair |
NEIL1 CRISPR/Cas9 KO Plasmid (h) - sc-403778 from Santa Cruz Biotechnology
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NEIL1 HDR Plasmid (h) - sc-403778-HDR from Santa Cruz Biotechnology
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NEIL1 Double Nickase Plasmid (h) - sc-403778-NIC from Santa Cruz Biotechnology
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NEIL1 Double Nickase Plasmid (h2) - sc-403778-NIC-2 from Santa Cruz Biotechnology
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NEIL1 CRISPR Activation Plasmid (h) - sc-403778-ACT from Santa Cruz Biotechnology
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NEIL1 CRISPR Activation Plasmid (h2) - sc-403778-ACT-2 from Santa Cruz Biotechnology
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NEIL1 Lentiviral Activation Particles (h) - sc-403778-LAC from Santa Cruz Biotechnology
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NEIL1 Lentiviral Activation Particles (h2) - sc-403778-LAC-2 from Santa Cruz Biotechnology
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NEIL1 siRNA (h) - sc-61166 from Santa Cruz Biotechnology
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NEIL1 shRNA Plasmid (h) - sc-61166-SH from Santa Cruz Biotechnology
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NEIL1 shRNA (h) Lentiviral Particles - sc-61166-V from Santa Cruz Biotechnology
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NEIL1 RNAi - H00079661-R01 from Novus Biologicals
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MISSION® esiRNA - EHU081311 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN15195 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND15195 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR00327 from Sigma-Aldrich
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NEIL1 siRNA (Human) - CRJ2466 from Cohesion Biosciences
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shRNA set against Human NEIL1 (NM_024608.3) - SHH351160 from Creative biogene
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Human NEIL1 siRNA - orb272967 from Biorbyt
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NEIL1 shRNA Plasmid (Human) - CHJ2466 from Cohesion Biosciences
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NEIL1 miRNA 3'UTR clone - MiUTR3H-10263 from Creative biogene
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