Gene Symbol | ALKBH1 |
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Entrez Gene | 8846 |
Alt Symbol | ABH, ABH1, ALKBH, alkB, hABH |
Species | Human |
Gene Type | protein-coding |
Description | alkB, alkylation repair homolog 1 (E. coli) |
Other Description | DNA lyase ABH1|DNA oxidative demethylase ALKBH1|alkylated DNA repair protein alkB homolog 1|alkylation repair, alkB homolog|alpha-ketoglutarate-dependent dioxygenase ABH1 |
Swissprots | Q9ULA7 Q13686 Q8TAU1 |
Accessions | AAF01478 EAW81300 EAW81301 EAW81302 Q13686 BC015024 AAH15024 BC025787 AAH25787 BX537699 DQ890543 ABM92160 DQ893703 ABM84629 X91992 CAA63047 XM_005268165 XP_005268222 NM_006020 NP_006011 NM_001039506 |
Function | Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron. {ECO:0000250, ECO:0000269|PubMed:18603530, ECO:0000269|PubMed:19959401}. |
Subcellular Location | Mitochondrion {ECO:0000269|PubMed:17979886, ECO:0000269|PubMed:18603530}. Nucleus {ECO:0000250}. Note=Mainly localizes in euchromatin, largely excluded from heterochromatin and nucleoli. {ECO:0000250}. |
Tissue Specificity | Ubiquitous. {ECO:0000269|PubMed:17979886, ECO:0000269|PubMed:18603530}. |
ALKB CRISPR/Cas9 KO Plasmid (h) - sc-408412 from Santa Cruz Biotechnology
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ALKB HDR Plasmid (h) - sc-408412-HDR from Santa Cruz Biotechnology
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ALKB Double Nickase Plasmid (h) - sc-408412-NIC from Santa Cruz Biotechnology
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ALKB Double Nickase Plasmid (h2) - sc-408412-NIC-2 from Santa Cruz Biotechnology
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ALKB CRISPR Activation Plasmid (h) - sc-408412-ACT from Santa Cruz Biotechnology
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ALKB CRISPR Activation Plasmid (h2) - sc-408412-ACT-2 from Santa Cruz Biotechnology
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ALKB Lentiviral Activation Particles (h) - sc-408412-LAC from Santa Cruz Biotechnology
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ALKB Lentiviral Activation Particles (h2) - sc-408412-LAC-2 from Santa Cruz Biotechnology
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ALKB siRNA (h) - sc-60153 from Santa Cruz Biotechnology
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ALKB shRNA Plasmid (h) - sc-60153-SH from Santa Cruz Biotechnology
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ALKB shRNA (h) Lentiviral Particles - sc-60153-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU135521 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN3066 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND3066 from Sigma-Aldrich
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MISSION® 3′UTR Lenti GoClone™ - HUTR09518 from Sigma-Aldrich
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ALKBH1 siRNA (Human) - CRH5841 from Cohesion Biosciences
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ALKBH1 - MBS8217842 from MyBioSource
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shRNA set against Human ALKBH1 (NM_006020.2) - SHH235214 from Creative biogene
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Human ALKBH1 siRNA - orb266343 from Biorbyt
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ALKBH1 shRNA Plasmid (Human) - CHH5841 from Cohesion Biosciences
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ALKBH1 miRNA 3'UTR clone - MiUTR1H-00306 from Creative biogene
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