UBE2W
| Gene Symbol | UBE2W |
|---|---|
| Entrez Gene | 55284 |
| Alt Symbol | UBC-16, UBC16 |
| Species | Human |
| Gene Type | protein-coding |
| Description | ubiquitin-conjugating enzyme E2W (putative) |
| Other Description | N-terminus-conjugating E2|ubiquitin carrier protein W|ubiquitin-conjugating enzyme 16|ubiquitin-conjugating enzyme 2W|ubiquitin-conjugating enzyme E2 W|ubiquitin-protein ligase W |
| Swissprots | B4DIV1 Q1XBE0 Q9NV07 Q9HAG6 Q96B02 Q9H823 |
| Accessions | EAW87009 EAW87010 EAW87011 EAW87012 Q96B02 AK001873 BAA91954 AK021735 BAB13883 AK024050 BAB14800 AK123531 AK225554 AK295792 BAG58613 AY948289 AAY24555 BC010900 AAH10900 BC016326 AAH16326 BU155172 BX538145 BX648427 CR457275 CAG33556 DA352184 EU446497 ABZ92026 NM_001001481 NP_001001481 NM_001271015 NP_001257944 NM_018299 NP_060769 NR_073119 NR_073120 NR_073121 NM_001001482 |
| Function | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Transfers ubiquitin in complex with RING/U-box type E3s that do not have active site cysteine residues to form thioester bonds with ubiquitin, and preferentially ubiquitinates the N-terminus of substrates, such as ATXN3, STUB1 and SUMO2. {ECO:0000269|PubMed:19111657, ECO:0 |
| Subcellular Location | Nucleus {ECO:0000269|PubMed:16368532}. Note=In the nucleus, colocalizes with FANCL. |
| Tissue Specificity | Widely expressed, with highest expression in brain, liver, pancreas and heart. {ECO:0000269|PubMed:16368532}. |
| Top Pathways | Ubiquitin mediated proteolysis |
UBE2W CRISPR/Cas9 KO Plasmid (h) - sc-418533 from Santa Cruz Biotechnology
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UBE2W HDR Plasmid (h) - sc-418533-HDR from Santa Cruz Biotechnology
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UBE2W Double Nickase Plasmid (h) - sc-418533-NIC from Santa Cruz Biotechnology
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UBE2W Double Nickase Plasmid (h2) - sc-418533-NIC-2 from Santa Cruz Biotechnology
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UBE2W CRISPR Activation Plasmid (h) - sc-418533-ACT from Santa Cruz Biotechnology
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UBE2W CRISPR Activation Plasmid (h2) - sc-418533-ACT-2 from Santa Cruz Biotechnology
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UBE2W Lentiviral Activation Particles (h) - sc-418533-LAC from Santa Cruz Biotechnology
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UBE2W Lentiviral Activation Particles (h2) - sc-418533-LAC-2 from Santa Cruz Biotechnology
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UBE2W siRNA (h) - sc-77861 from Santa Cruz Biotechnology
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UBE2W shRNA Plasmid (h) - sc-77861-SH from Santa Cruz Biotechnology
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UBE2W shRNA (h) Lentiviral Particles - sc-77861-V from Santa Cruz Biotechnology
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UBE2W RNAi - H00055284-R03 from Novus Biologicals
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UBE2W RNAi - H00055284-R02 from Novus Biologicals
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UBE2W RNAi - H00055284-R01 from Novus Biologicals
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MISSION® esiRNA - EHU077461 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN22390 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND22390 from Sigma-Aldrich
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UBE2W siRNA (Human) - CRJ0624 from Cohesion Biosciences
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UBE2W - MBS8207302 from MyBioSource
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shRNA set against Human UBE2W (NM_018299.4) - SHH439232 from Creative biogene
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UBE2W shRNA Plasmid (Human) - CHJ0624 from Cohesion Biosciences
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