CHST15

Gene Symbol	CHST15
Entrez Gene	51363
Alt Symbol	BRAG, GALNAC4S-6ST
Species	Human
Gene Type	protein-coding
Description	carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15
Other Description	B cell RAG associated protein (GALNAC4S-6ST)\|B-cell RAG-associated gene protein\|carbohydrate sulfotransferase 15\|hBRAG
Swissprots	Q86VM4 O60474 O60338 Q7LFX5
Accessions	BAA83686 EAW49273 EAW49274 EAW49275 EAW49276 Q7LFX5 AB011170 BAA25524 AB062423 BAB72145 AF026477 AAC71691 AK294961 BAG58035 AK299546 BAG61492 BC027908 AAH27908 BC050540 AAH50540 BC075813 AAH75813 BM193389 BM977716 CR749804 CAH18664 XM_005269891 XP_005269948 XM_005269892 XP_005269949 XM_005269893 XP_005269950 XM_005269894 XP_005269951 XM_006717891 XP_006717954 XM_011539857 XP_011538159 XR_945773 NM_001270764 NP_001257693 NM_001270765 NP_001257694 NM_014863 NP_055678 NM_015892 NP_056976
Function	Sulfotransferase that transfers sulfate from 3'- phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non- reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B- cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo. {ECO:0000269\|PubMed:11572857, ECO:0000269\|PubMed:12874280}.
Subcellular Location	Golgi apparatus membrane {ECO:0000305\|PubMed:10749872}; Single-pass type II membrane protein {ECO:0000305\|PubMed:10749872}. Note=A small fraction may also be present at the cell surface, where it acts as a B-cell receptor.
Tissue Specificity	Expressed in B-cell-enriched tissues but not in fetal or adult thymus. Expressed in fetal and adult spleen, lymph node, tonsil, bone marrow and peripheral leukocytes. Not expressed in T-cells. In pro-B, pre-B, and mature B-cell lines, it colocalizes with RAG1. {ECO:0000269\|PubMed:9754571}.
Top Pathways	Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate

Human BRAG gene - orb91575 from Biorbyt

Prices	$519.00
Sizes	10 μg
Species	Human

Human-GALNAC4S-6ST-cDNA-Clone - DC08966 from Abgent, a WuXi AppTec company

Prices	$757.35
Sizes	10 µg
Species	Human
Accession	BC075813

Human CHST15 ORF Clone(NM_014863.3) - CDCR298396 from Creative Biomart

Species	Human
Accession	NM_014863.3

Human CHST15 ORF Clone(NM_014863.3) - CDCR298397 from Creative Biomart

Species	Human
Accession	NM_014863.3

Human GALNAC4S-6ST/CHST15 Gene ORF cDNA clone in cloning vector - HG11033-M from Sino Biological

Species	Human

Human GALNAC4S-6ST/CHST15 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag - HG11033-ACG from Sino Biological

Species	Human

Human GALNAC4S-6ST/CHST15 Gene ORF cDNA clone expression plasmid, C-His tag - HG11033-CH from Sino Biological

Species	Human

Human GALNAC4S-6ST/CHST15 Gene ORF cDNA clone expression plasmid, N-Flag tag - HG11033-NF from Sino Biological

Species	Human

Human GALNAC4S-6ST/CHST15 Gene ORF cDNA clone expression plasmid, N-Myc tag - HG11033-NM from Sino Biological

Species	Human

Human GALNAC4S-6ST/CHST15 Gene ORF cDNA clone expression plasmid, C-Flag tag - HG11033-CF from Sino Biological

Species	Human

Human CHST15 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged - HG11033-M-N from Sino Biological

Species	Human
Accession	NM_015892.2

Human GALNAC4S-6ST/CHST15 Gene ORF cDNA clone expression plasmid, C-Myc tag - HG11033-CM from Sino Biological

Species	Human

Human GALNAC4S-6ST/CHST15 Gene ORF cDNA clone expression plasmid, C-HA tag - HG11033-CY from Sino Biological

Species	Human

Human GALNAC4S-6ST/CHST15 Gene ORF cDNA clone expression plasmid, N-HA tag - HG11033-NY from Sino Biological

Species	Human

Human GALNAC4S-6ST/CHST15 Gene ORF cDNA clone expression plasmid, C-Flag tag - HG11033-M-F from Sino Biological

Species	Human

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

Human GALNAC4S-6ST/CHST15 Gene ORF cDNA clone expression plasmid, N-His tag - HG11033-NH from Sino Biological

Species	Human

Human GALNAC4S-6ST/CHST15 Gene ORF cDNA clone expression plasmid - HG11033-UT from Sino Biological

Species	Human

Human CHST15 ORF Clone(NM_014863.3) - CDCR298396 from Creative biogene

Species	Human

Human CHST15 ORF Clone (BC075813) - CDCS418338 from Creative biogene

Species	Human
Accession	Q7LFX5

Human CHST15 ORF clone (NM_015892.2) - CDCB157477 from Creative biogene

Species	Human

Human GALNAC4S-6ST/CHST15 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag - HG11033-ACR from Sino Biological

Species	Human

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