| Gene Symbol | CHST15 |
|---|---|
| Entrez Gene | 51363 |
| Alt Symbol | BRAG, GALNAC4S-6ST |
| Species | Human |
| Gene Type | protein-coding |
| Description | carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15 |
| Other Description | B cell RAG associated protein (GALNAC4S-6ST)|B-cell RAG-associated gene protein|carbohydrate sulfotransferase 15|hBRAG |
| Swissprots | Q86VM4 O60474 O60338 Q7LFX5 |
| Accessions | BAA83686 EAW49273 EAW49274 EAW49275 EAW49276 Q7LFX5 AB011170 BAA25524 AB062423 BAB72145 AF026477 AAC71691 AK294961 BAG58035 AK299546 BAG61492 BC027908 AAH27908 BC050540 AAH50540 BC075813 AAH75813 BM193389 BM977716 CR749804 CAH18664 XM_005269891 XP_005269948 XM_005269892 XP_005269949 XM_005269893 XP_005269950 XM_005269894 XP_005269951 XM_006717891 XP_006717954 XM_011539857 XP_011538159 XR_945773 NM_001270764 NP_001257693 NM_001270765 NP_001257694 NM_014863 NP_055678 NM_015892 NP_056976 |
| Function | Sulfotransferase that transfers sulfate from 3'- phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non- reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B- cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo. {ECO:0000269|PubMed:11572857, ECO:0000269|PubMed:12874280}. |
| Subcellular Location | Golgi apparatus membrane {ECO:0000305|PubMed:10749872}; Single-pass type II membrane protein {ECO:0000305|PubMed:10749872}. Note=A small fraction may also be present at the cell surface, where it acts as a B-cell receptor. |
| Tissue Specificity | Expressed in B-cell-enriched tissues but not in fetal or adult thymus. Expressed in fetal and adult spleen, lymph node, tonsil, bone marrow and peripheral leukocytes. Not expressed in T-cells. In pro-B, pre-B, and mature B-cell lines, it colocalizes with RAG1. {ECO:0000269|PubMed:9754571}. |
| Top Pathways | Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate |
GalNAc4S-6ST CRISPR/Cas9 KO Plasmid (h) - sc-407793 from Santa Cruz Biotechnology
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GalNAc4S-6ST HDR Plasmid (h) - sc-407793-HDR from Santa Cruz Biotechnology
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GalNAc4S-6ST Double Nickase Plasmid (h) - sc-407793-NIC from Santa Cruz Biotechnology
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GalNAc4S-6ST Double Nickase Plasmid (h2) - sc-407793-NIC-2 from Santa Cruz Biotechnology
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GalNAc4S-6ST CRISPR Activation Plasmid (h) - sc-407793-ACT from Santa Cruz Biotechnology
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GalNAc4S-6ST CRISPR Activation Plasmid (h2) - sc-407793-ACT-2 from Santa Cruz Biotechnology
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GalNAc4S-6ST Lentiviral Activation Particles (h) - sc-407793-LAC from Santa Cruz Biotechnology
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GalNAc4S-6ST Lentiviral Activation Particles (h2) - sc-407793-LAC-2 from Santa Cruz Biotechnology
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GalNAc4S-6ST siRNA (h) - sc-90417 from Santa Cruz Biotechnology
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GalNAc4S-6ST shRNA Plasmid (h) - sc-90417-SH from Santa Cruz Biotechnology
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GalNAc4S-6ST shRNA (h) Lentiviral Particles - sc-90417-V from Santa Cruz Biotechnology
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CompoZr® Knockout ZFN Kit - CKOZFN6109 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND6109 from Sigma-Aldrich
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CHST15 siRNA (Human) - CRH9722 from Cohesion Biosciences
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CHST15 - MBS828502 from MyBioSource
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shRNA set against Human CHST15 (NM_015892.4) - SHH263777 from Creative biogene
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Human CHST15 siRNA - orb270224 from Biorbyt
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CHST15 shRNA Plasmid (Human) - CHH9722 from Cohesion Biosciences
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CHST15 miRNA 3'UTR clone - MiUTR4H-TG02107 from Creative biogene
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