Gene Symbol | NTAN1 |
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Entrez Gene | 123803 |
Alt Symbol | PNAA, PNAD |
Species | Human |
Gene Type | protein-coding |
Description | N-terminal asparagine amidase |
Other Description | protein N-terminal Asn amidase|protein N-terminal asparagine amidase|protein N-terminal asparagine amidohydrolase|protein NH2-terminal asparagine deamidase|protein NTN-amidase |
Swissprots | Q7Z4Z0 Q96AB6 |
Accessions | EAW54749 Q96AB6 AF092440 AAP97215 AK092915 BC017336 AAH17336 BF939373 DC383993 HQ447215 ADQ31702 HY036316 XM_011522355 XP_011520657 XM_011546694 XP_011544996 NM_001270766 NP_001257695 NM_001270767 NP_001257696 NM_173474 NP_775745 |
Function | Side-chain deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N- terminal asparagine to aspartate by PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position, nor on acetylated N- terminal peptidyl Asn. {ECO:0000269|PubMed:21375249}. |
Subcellular Location | Cytoplasm {ECO:0000250}. |
PNAd CRISPR/Cas9 KO Plasmid (h) - sc-410272 from Santa Cruz Biotechnology
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PNAd HDR Plasmid (h) - sc-410272-HDR from Santa Cruz Biotechnology
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PNAd Double Nickase Plasmid (h) - sc-410272-NIC from Santa Cruz Biotechnology
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PNAd Double Nickase Plasmid (h2) - sc-410272-NIC-2 from Santa Cruz Biotechnology
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PNAd CRISPR Activation Plasmid (h) - sc-410272-ACT from Santa Cruz Biotechnology
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PNAd CRISPR Activation Plasmid (h2) - sc-410272-ACT-2 from Santa Cruz Biotechnology
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PNAd Lentiviral Activation Particles (h) - sc-410272-LAC from Santa Cruz Biotechnology
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PNAd Lentiviral Activation Particles (h2) - sc-410272-LAC-2 from Santa Cruz Biotechnology
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PNAd siRNA (h) - sc-62832 from Santa Cruz Biotechnology
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PNAd shRNA Plasmid (h) - sc-62832-SH from Santa Cruz Biotechnology
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PNAd shRNA (h) Lentiviral Particles - sc-62832-V from Santa Cruz Biotechnology
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MISSION® esiRNA - EHU010681 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit - CKOZFN15557 from Sigma-Aldrich
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CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND15557 from Sigma-Aldrich
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NTAN1 siRNA (Human) - CRJ4741 from Cohesion Biosciences
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shRNA set against Human NTAN1 (NM_173474.3) - SHH355640 from Creative biogene
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Human NTAN1 siRNA - orb275242 from Biorbyt
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NTAN1 shRNA Plasmid (Human) - CHJ4741 from Cohesion Biosciences
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NTAN1 miRNA 3'UTR clone - MiUTR3H-15617 from Creative biogene
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