NTAN1

Gene Symbol NTAN1
Entrez Gene 123803
Alt Symbol PNAA, PNAD
Species Human
Gene Type protein-coding
Description N-terminal asparagine amidase
Other Description protein N-terminal Asn amidase|protein N-terminal asparagine amidase|protein N-terminal asparagine amidohydrolase|protein NH2-terminal asparagine deamidase|protein NTN-amidase
Swissprots Q7Z4Z0 Q96AB6
Accessions EAW54749 Q96AB6 AF092440 AAP97215 AK092915 BC017336 AAH17336 BF939373 DC383993 HQ447215 ADQ31702 HY036316 XM_011522355 XP_011520657 XM_011546694 XP_011544996 NM_001270766 NP_001257695 NM_001270767 NP_001257696 NM_173474 NP_775745
Function Side-chain deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N- terminal asparagine to aspartate by PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position, nor on acetylated N- terminal peptidyl Asn. {ECO:0000269|PubMed:21375249}.
Subcellular Location Cytoplasm {ECO:0000250}.

PNAd (S-19) - sc-54991 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PNAd (S-19): sc-54991. Western blot analysis of PNAd expression in non-transfected: sc-117752 (A) and human PNAd transfected: sc-370403 (B) 293T whole cell lysates.

PNAd (H-140) - sc-67404 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

PNAd (E-11) - sc-166493 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PNAd (E-11): sc-166493. Western blot analysis of PNAd expression in Hep G2 (A) and WI 38 (B) whole cell lysates.

PNAd (L-20) - sc-54990 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PNAd (E-17) - sc-54989 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PNAd (C-14) - sc-54988 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-NTAN1 antibody - ab111710 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-NTAN1 antibody (ab111710) at 1/1000 dilution + 293T whole cell lysate at 30 µg

Anti-NTAN1 antibody - ab197324 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-NTAN1 antibody (ab197324) at 1/500 dilution + HepG2 cell lysate

Anti-NTAN1 antibody - ab67472 from Abcam

Prices $370.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
All lanes : Anti-NTAN1 antibody (ab67472) at 1/500 dilutionLane 1 : NTAN1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg/ml per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-NTAN1 antibody - C-terminal - ab173565 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-NTAN1 antibody - C-terminal (ab173565) at 1/100 dilution + T47D cell line lysate at 35 µg

Anti-NTAN1 / PNAD Antibody (aa203-230) - LS-C158428 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
NTAN1 Antibody western blot of T47D cell line lysates (35 ug/lane). The NTAN1 antibody detected the NTAN1 protein (arrow).

Anti-NTAN1 / PNAD Antibody (aa202-230, AP) - LS-C238188 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NTAN1 / PNAD Antibody (aa202-230, APC) - LS-C238189 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NTAN1 / PNAD Antibody (aa202-230, Biotin) - LS-C238191 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NTAN1 / PNAD Antibody (aa202-230, PE) - LS-C238193 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NTAN1 / PNAD Antibody (aa202-230, FITC) - LS-C238192 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NTAN1 / PNAD Antibody (aa202-230, Azide-free, HRP) - LS-C248957 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-NTAN1 / PNAD Antibody (aa202-230) - LS-C329918 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

NTAN1 Antibody - NBP2-33615 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: NTAN1 Antibody [NBP2-33615] - pancreas

NTAN1 Antibody - NBP2-15046 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NTAN1 Antibody [NBP2-15046] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE; antibody diluted at 1:1000.

NTAN1 Antibody - H00123803-B01 from Novus Biologicals

Prices $349.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot analysis of NTAN1 expression in transfected 293T cell line by NTAN1 MaxPab polyclonal antibody (B01). Lane 1: NTAN1 transfected lysate (34.70 KDa). Lane 2: Non-transfected lysate.

Anti-NTAN1 (293-305) antibody produced in rabbit - SAB1102874 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human NTAN1 was separated on SDS-PAGE, blotted with Anti-NTAN1 (293-305) (<B>Cat. No. SAB1102874</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:1,000

Anti-NTAN1 antibody produced in rabbit - SAB2701408 from Sigma-Aldrich

Prices $326.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-NTAN1: <B>Cat. No. SAB2701408</B>: Immunohistochemical analysis of paraffin-embedded Hepatoma, using NTAN1 antibody at 1:500 dilution.

Anti-NTAN1 antibody produced in rabbit - HPA051418 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-NTAN1: <B>Cat. No. HPA051418</B>: Immunohistochemical staining of Human lung shows strong cytoplasmic positivity in pneumocytes.

Anti-NTAN1 (187-200) antibody produced in rabbit - SAB1102873 from Sigma-Aldrich

Prices $298.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human NTAN1 was separated on SDS-PAGE, blotted with Anti-NTAN1 (187-200) (<B>Cat. No.SAB1102873</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500<BR/>2. Antibody dilution 1:500 + NTAN1 immunizing peptide (human, 187-200)
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