PLN

Gene Symbol	PLN
Entrez Gene	5350
Alt Symbol	CMD1P, CMH18, PLB
Species	Human
Gene Type	protein-coding
Description	phospholamban
Other Description	cardiac phospholamban
Swissprots	P26678
Accessions	AAD55950 EAW48198 P26678 AK311799 BAG34742 BC005269 AAH05269 BP259965 DQ893034 ABM83960 DQ896146 ABM87145 M60411 AAA60109 M63603 AAA60083 NM_002667 NP_002658
Function	Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation. {ECO:0000269\|PubMed:22427649, ECO:0000269\|PubMed:22707725}.
Subcellular Location	Sarcoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane {ECO:0000250}; Single- pass membrane protein {ECO:0000250}. Endoplasmic reticulum membrane; Single-pass membrane protein.
Tissue Specificity	Heart muscle (at protein level). {ECO:0000269\|PubMed:17241641}.
Top Pathways	Calcium signaling pathway, Dilated cardiomyopathy, Adrenergic signaling in cardiomyocytes, cGMP-PKG signaling pathway, cAMP signaling pathway

Phospho-Phospholamban (Ser16/Thr17) Antibody - 8496 from Cell Signaling Technology

Prices	$287.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of extracts from 16-month old control (WKY) and spontaneous hypertensive (SHR) rat hearts using Phospho-Phospholamban (Ser16/Thr17) Antibody (left), Phospholamban Antibody #8495 (middle), or GAPDH (14C10) Rabbit mAb #2118 (right).

Phospholamban Antibody - 8495 from Cell Signaling Technology

Prices	$239.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Polyclonal

phospholamban (F-7) - sc-393990 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Mouse
Clonality	Monoclonal

phospholamban (F-7): sc-393990. Western blot analysis of phospholamban expression in mouse heart (A) and human heart (B) tissue extracts.

phospholamban (L-15) - sc-21923 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

phospholamban (L-15): sc-21923. Western blot analysis of phospholamban expression in rat heart (A) and mouse heart (B) tissue extracts.

phospholamban (FL-52) - sc-30142 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

p-phospholamban (Ser 16) - sc-12963 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Western blot analysis of phospholamban phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) mouse heart tissue extract. Antibodies tested include p-phospholamban (Ser 16)-R: sc-12963-R (A,B) and phospholamban (L-15): sc-21923 (C,D).

Anti-Phospholamban (phospho S16 + T17) antibody - ab62170 from Abcam

Prices	$370.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Immunofluorescence analysis of HuvEc cells, using Phospholamban (phospho S16 + T17) antibody (ab62170) at 1/500 - 1/1000 dilution, in the presence (right panel) and absence (left panel) of immunising phosphopeptide.

Anti-Phospholamban (phospho S16) antibody - ab15000 from Abcam

Prices	$394.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Phospholamban (phospho S16) antibody (ab15000) at 0.5 µg/mlLane 1 : Bovine cardiac preparation treated with PKALane 2 : Untreated bovine cardiac preparation

Anti-Phospholamban (phospho S16) antibody [EPR1911] - ab92697 from Abcam

Prices	$378.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR1911

All lanes : Anti-Phospholamban (phospho S16) antibody [EPR1911] (ab92697) at 1/200000 dilutionLane 1 : Fetal heart lysate (untreated)Lane 2 : Fetal heart lysate (treated with LP)Lysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti-Rabbit antibody at 1/2000 dilution

Anti-Phospholamban antibody - ab85146 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Phospholamban antibody (ab85146) at 1 µg/mlLane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 2 : Heart (Mouse) Tissue LysateLane 3 : Heart (Rat) Tissue Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Phospholamban antibody - ab126174 from Abcam

Prices	$376.00
Sizes	50 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Phospholamban antibody (ab126174) at 1/5000 dilution + Mouse heart whole cell lysate at 50 µg

Anti-Phospholamban antibody - ab103316 from Abcam

Prices	$359.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-Phospholamban antibody (ab103316) at 1/100 dilution + A375 cell lysate at 35 µg

Anti-Phospholamban antibody - ab103237 from Abcam

Prices	$376.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Anti-Phospholamban antibody (ab103237) at 1/100 dilution + Jurkat cell line lysate at 35 µg

Anti-Phospholamban antibody - ab86930 from Abcam

Prices	$376.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

All lanes : Anti-Phospholamban antibody (ab86930) at 1 µg/mlLane 1 : Rat heart tissue lysateLane 2 : Rat heart tissue lysate

Anti-Phospholamban antibody [2D12] - ab2865 from Abcam

Prices	$359.00
Sizes	100 µl
Host	Mouse
Clonality	Monoclonal
Clone	2D12

Ab2865 staining human normal liver. Staining is localized to the cytoplasm.Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-PLN / Phospholamban Antibody - LS-C38228 from LifeSpan Bioscience

Host	Mouse
Clonality	Monoclonal

Anti-PLN / Phospholamban Antibody (clone A1) - LS-C85366 from LifeSpan Bioscience

Host	Mouse
Clonality	Monoclonal
Clone	A1

Anti-PLN / Phospholamban Antibody (phospho-Ser16) - LS-C38229 from LifeSpan Bioscience

Prices	$590.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PLN / Phospholamban Antibody (N-Terminus) - LS-C131403 from LifeSpan Bioscience

Prices	$405.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PLN / Phospholamban Antibody (N-Terminus, Magnetic beads) - LS-C171975 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PLN / Phospholamban Antibody (phospho-Thr17) - LS-C163663 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Dot blot of anti-Phospho-PLB-T17 Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Anti-PLN / Phospholamban Antibody (phospho-Ser16, clone EPR1911) - LS-C105664 from LifeSpan Bioscience

Prices	$475.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR1911

Western blot analysis on fetal heart lysates using anti-Phospholamban Phospho (pS16) antibody (LS-C105664). Cells were either (A) untreated (B) treated with LP.

Anti-PLN / Phospholamban Antibody (N-Terminus, Sepharose) - LS-C171976 from LifeSpan Bioscience

Prices	$325.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-PLN / Phospholamban Antibody (AP) - LS-C266479 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-PLN / Phospholamban Antibody (APC) - LS-C266480 from LifeSpan Bioscience

Prices	$545.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

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