SMPD3

Gene Symbol SMPD3
Entrez Gene 55512
Alt Symbol NSMASE2
Species Human
Gene Type protein-coding
Description sphingomyelin phosphodiesterase 3, neutral membrane (neutral sphingomyelinase II)
Other Description nSMase-2|neutral sphingomyelinase 2|neutral sphingomyelinase II|sphingomyelin phosphodiesterase 3
Swissprots B7ZL82 Q9NY59 Q2M1S8
Accessions ACV30039 ADG64729 ADG64730 CAD35076 CCF76927 EAW83233 Q9NY59 AA461272 AJ250460 CAB92964 AK026246 BAB15409 AK289651 BAF82340 AL157660 AW163364 BC012727 BC041828 BC112238 AAI12239 BC143631 AAI43632 BC143634 AAI43635 BI196095 CA424980 CB268111 DQ424899 ABD83664 HQ258353 ADR83107 XM_005256031 XP_005256088 XM_005256032 XP_005256089 XM_011523207 XP_011521509 XM_011523208 XP_011521510 XM_011523209 XP_011521511 XM_011523210 XP_011521512 XR_933371 NM_018667 NP_061137
Function Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization. {ECO:0000269|PubMed:10823942, ECO:0000269|PubMed:14741383, ECO:0000269|PubMed:15051724}.
Subcellular Location Golgi apparatus membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Note=May localize to detergent-resistant subdomains of Golgi membranes of hypothalamic neurosecretory neurons. According to PubMed:15051724, it localizes to plasma membrane in confluent contact-inhibited cells.
Tissue Specificity Predominantly expressed in brain. {ECO:0000269|PubMed:10823942}.
Top Pathways Sphingolipid metabolism

N-SMase2 (H-195) - sc-67305 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
N-SMase2 (H-195): sc-67305. Western blot analysis of N-SMase2 expression in PC-12 whole cell lysate.

N-SMase2 (I-16) - sc-67692 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
N-SMase2 (I-16): sc-67692. Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells.

N-SMase2 (G-6) - sc-166637 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
N-SMase2 (G-6): sc-166637. Western blot analysis of N-SMase2 expression in non-transfected: sc-117752 (A) and mouse N-SMase2 transfected: sc-121910 (B) 293T whole cell lysates.

N-SMase2 (N-17) - sc-67693 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
N-SMase2 (N-17): sc-67693. Western blot analysis of N-SMase2 expression in HEK293 (A), Hep G2 (B), CCRF-CEM (C), HeLa (D), Jurkat (E) and K-562 (F) whole cell lysates.

Anti-NSMase2 antibody - ab85017 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-NSMase2 antibody (ab85017) at 1 µg/ml + Brain (Rat) Tissue Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-NSMase2 antibody - ab199399 from Abcam

Prices $359.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Anti-NSMase2 antibody (ab199399) at 1/500 dilution + Human fetal brain lysate

Anti-NSMase2 antibody - ab68735 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-NSMase2 antibody (ab68735) at 1/500 dilutionLane 1 : nSMase2 transfected 293T cell lysateLane 2 : Non transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-NSMase2 antibody - ab172793 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NSMase2 antibody (ab172793) at 1 µg/mlLane 1 : NSMase2 transfected 293T cell line lysateLane 2 : Non-transfected 293T cell line lysateLysates/proteins at 15 µl per lane.

Anti-SMPD3 / NSMASE2 Antibody (aa137-165, AP) - LS-C241017 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMPD3 / NSMASE2 Antibody (aa137-165, APC) - LS-C241018 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMPD3 / NSMASE2 Antibody (aa137-165, FITC) - LS-C241020 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMPD3 / NSMASE2 Antibody (aa254-303) - LS-C81364 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Fetal lung lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-SMPD3 / NSMASE2 Antibody (aa137-165, Biotin) - LS-C241021 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMPD3 / NSMASE2 Antibody (aa137-165, Azide-free, HRP) - LS-C248205 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMPD3 / NSMASE2 Antibody (aa137-165, PE) - LS-C241022 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMPD3 / NSMASE2 Antibody (aa137-165) - LS-C330165 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMPD3 / NSMASE2 Antibody - LS-C140613 from LifeSpan Bioscience

Prices $460.00
Sizes 10 blot
Host Rabbit
Clonality Polyclonal
Western blot of affinity purified nSMase2 antibody (LS-C140613) on human brain lysate. Antibody without (lane 1) or with (lane 2) blocking peptide. Visualized using mouse anti-rabbit HRP conjugated at 1:200K dilution).

Anti-SMPD3 / NSMASE2 Antibody - LS-C140614 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of nSMase2 antibody (LS-C140614) on human brain lysate. Lysate used at 14 ug/lane. Antibody used at 10

Anti-SMPD3 / NSMASE2 Antibody (aa137-165) - LS-C159044 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
SMPD3 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The SMPD3 antibody detected the SMPD3 protein (arrow).

SMPD3/NSMase2 Antibody (758612) [Unconjugated] - MAB7184 from Novus Biologicals

Prices $99.00, $109.00, $249.00, $274.00
Sizes 25 µg, 25 µg, 100 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 758612
Western blot shows lysates of CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SMPD3 Monoclonal Antibody (Catalog # MAB7184) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SMPD3 at approximately 70-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

SMPD3/NSMase2 Antibody - AF7184 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal
Western blot shows lysates of RPMI 8226 human multiple myeloma cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human SMPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7184) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for SMPD3 at approximately 70 to 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

SMPD3/NSMase2 Antibody - NBP1-69344 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: NSMASE2 Antibody [NBP1-69344] - This Anti-SMPD3 antibody was used in Western Blot of Fetal Lung tissue lysate at a concentration of 1ug/ml.

SMPD3/NSMase2 Antibody - H00055512-D01P from Novus Biologicals

Prices $399.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: SMPD3/NSMase2 Antibody [H00055512-D01P] - Analysis of SMPD3 expression in transfected 293T cell line by SMPD3 polyclonal antibody.Lane 1: SMPD3 transfected lysate(71.10 KDa).Lane 2: Non-transfected lysate.

SMPD3/NSMase2 Antibody - H00055512-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

SMPD3/NSMase2 Antibody - H00055512-D01 from Novus Biologicals

Prices $399.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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