DDX58
| Gene Symbol | DDX58 |
|---|---|
| Entrez Gene | 23586 |
| Alt Symbol | RIG-I, RIGI, RLR-1, SGMRT2 |
| Species | Human |
| Gene Type | protein-coding |
| Description | DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 |
| Other Description | DEAD box protein 58|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide|RNA helicase RIG-I|probable ATP-dependent RNA helicase DDX58|retinoic acid-inducible gene 1 protein|retinoic acid-inducible gene I protein |
| Swissprots | Q5VYT1 O95786 Q9NT04 Q5HYE1 A2RU81 |
| Accessions | CCQ43430 EAW58547 EAW58548 O95786 AA862613 AF038963 AAD19826 AK023661 BAG51213 AK125989 BAG54273 AK301678 BAG63151 AK315040 AL137608 CAB70840 BC070029 BC107731 AAI07732 BC132786 AAI32787 BC136610 AAI36611 BX647917 CAI46068 DN998145 NM_014314 NP_055129 |
| Function | Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include: 5'- triphosphorylated ssRNA and dsRNA and short dsRNA (<1 kb in length). In addition to the 5'-triphosphate moiety, blunt-end base pairing at the 5'-end of the RNA is very essential. Overhangs at the non-triphosphorylated end of the dsRNA RNA have no major impact on its activity. A 3'overhang at the 5'triphosphate end decreases and any 5'overhang at the 5' triphosphate end abolishes its activity. Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK- related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs) |
| Subcellular Location | Cytoplasm. Cell projection, ruffle membrane. Cytoplasm, cytoskeleton. Cell junction, tight junction. Note=Colocalized with TRIM25 at cytoplasmic perinuclear bodies. Associated with the actin cytoskeleton at membrane ruffles. |
| Tissue Specificity | Present in vascular smooth cells (at protein level). {ECO:0000269|PubMed:15219805}. |
| Top Pathways | RIG-I-like receptor signaling pathway, Cytosolic DNA-sensing pathway, Hepatitis C, Measles, Influenza A |
RIG-I CRISPR/Cas9 KO Plasmid (h) - sc-400812 from Santa Cruz Biotechnology
|
||||||||
RIG-I HDR Plasmid (h) - sc-400812-HDR from Santa Cruz Biotechnology
|
||||||||
RIG-I Double Nickase Plasmid (h) - sc-400812-NIC from Santa Cruz Biotechnology
|
||||||||
RIG-I Double Nickase Plasmid (h2) - sc-400812-NIC-2 from Santa Cruz Biotechnology
|
||||||||
RIG-I CRISPR Activation Plasmid (h) - sc-400812-ACT from Santa Cruz Biotechnology
|
||||||||
RIG-I CRISPR Activation Plasmid (h2) - sc-400812-ACT-2 from Santa Cruz Biotechnology
|
||||||||
RIG-I Lentiviral Activation Particles (h) - sc-400812-LAC from Santa Cruz Biotechnology
|
||||||||
RIG-I Lentiviral Activation Particles (h2) - sc-400812-LAC-2 from Santa Cruz Biotechnology
|
||||||||
RIG-I siRNA (h) - sc-61480 from Santa Cruz Biotechnology
|
||||||||
RIG-I shRNA Plasmid (h) - sc-61480-SH from Santa Cruz Biotechnology
|
||||||||
RIG-I shRNA (h) Lentiviral Particles - sc-61480-V from Santa Cruz Biotechnology
|
||||||||
RIG-I RNAi - H00023586-R02 from Novus Biologicals
|
||||||||
MISSION® esiRNA - EHU131051 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit - CKOZFN2394 from Sigma-Aldrich
|
||||||||
CompoZr® Knockout ZFN Kit, ZFN plasmid only - CKOZFND2394 from Sigma-Aldrich
|
||||||||
MISSION® 3′UTR Lenti GoClone™ - HUTR10343 from Sigma-Aldrich
|
||||||||
DDX58 siRNA (Human) - CRH8377 from Cohesion Biosciences
|
||||||||
DDX58 - MBS8236537 from MyBioSource
|
||||||||
RIG-I siRNA (r) - sc-270587 from Santa Cruz Biotechnology
|
||||||||
RIG-I shRNA Plasmid (r) - sc-270587-SH from Santa Cruz Biotechnology
|
||||||||
RIG-I shRNA (r) Lentiviral Particles - sc-270587-V from Santa Cruz Biotechnology
|
||||||||
shRNA set against Human DDX58 (NM_014314.3) - SHH276173 from Creative biogene
|
||||||||
shRNA set against Human DDX58(NM_014314.3) - SHG227341 from Creative biogene
|
||||||||
Human DDX58 siRNA - orb268879 from Biorbyt
|
||||||||
DDX58 shRNA Plasmid (Human) - CHH8377 from Cohesion Biosciences
|