DDX58

Gene Symbol DDX58
Entrez Gene 23586
Alt Symbol RIG-I, RIGI, RLR-1, SGMRT2
Species Human
Gene Type protein-coding
Description DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
Other Description DEAD box protein 58|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide|RNA helicase RIG-I|probable ATP-dependent RNA helicase DDX58|retinoic acid-inducible gene 1 protein|retinoic acid-inducible gene I protein
Swissprots Q5VYT1 O95786 Q9NT04 Q5HYE1 A2RU81
Accessions CCQ43430 EAW58547 EAW58548 O95786 AA862613 AF038963 AAD19826 AK023661 BAG51213 AK125989 BAG54273 AK301678 BAG63151 AK315040 AL137608 CAB70840 BC070029 BC107731 AAI07732 BC132786 AAI32787 BC136610 AAI36611 BX647917 CAI46068 DN998145 NM_014314 NP_055129
Function Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include: 5'- triphosphorylated ssRNA and dsRNA and short dsRNA (<1 kb in length). In addition to the 5'-triphosphate moiety, blunt-end base pairing at the 5'-end of the RNA is very essential. Overhangs at the non-triphosphorylated end of the dsRNA RNA have no major impact on its activity. A 3'overhang at the 5'triphosphate end decreases and any 5'overhang at the 5' triphosphate end abolishes its activity. Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK- related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs)
Subcellular Location Cytoplasm. Cell projection, ruffle membrane. Cytoplasm, cytoskeleton. Cell junction, tight junction. Note=Colocalized with TRIM25 at cytoplasmic perinuclear bodies. Associated with the actin cytoskeleton at membrane ruffles.
Tissue Specificity Present in vascular smooth cells (at protein level). {ECO:0000269|PubMed:15219805}.
Top Pathways RIG-I-like receptor signaling pathway, Cytosolic DNA-sensing pathway, Hepatitis C, Measles, Influenza A

Rig-I (D33H10) Rabbit mAb - 4200 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D33H10
Western blot analysis of COS-7 cells, untransfected (-) or transfected (+) with human Rig-I, using Rig-I (D33H10) Rabbit mAb.

Rig-I (D14G6) Rabbit mAb - 3743 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D14G6
Western blot analysis of differentiated THP-1 and RAW 264.7 cells, untreated or LPS-treated (1 ug/ml, overnight), using Rig-I (D14G6) Rabbit mAb.

Rig-I Antibody - 4520 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells, mock transfected or transfected with Rig-I, and from differentiated THP-1 cells, untreated or treated with LPS, using Rig-I Antibody.

RIG-I (N-14) - sc-48932 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RIG-I (N-14): sc-48932. Immunoperoxidase staining of formalin fixed, paraffin-embedded human smooth muscle tissue showing cytoplasmic staining of smooth muscle cells.

RIG-I (H-300) - sc-98911 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
RIG-I (H-300): sc-98911. Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of Islets of Langerhans and glandular cells.

RIG-I (C-15) - sc-48929 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RIG-I (C-15): sc-48929. Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing cytoplasmic staining of cells in germinal centers and cells in non-germinal centers.

RIG-I (L-15) - sc-48931 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
RIG-I (L-15): sc-48931. Western blot analysis of RIG-I expression in SK-MEL-28 whole cell lysate.

Anti-DDX58 antibody - ab45428 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-DDX58 antibody (ab45428) at 0.5 µg/mlLane 2 : Anti-DDX58 antibody (ab45428) at 1 µg/mlLane 3 : Anti-DDX58 antibody (ab45428) at 2 µg/mlLane 1 : C2C12 cell lysateLane 2 : C2C12 cell lysateLane 3 : C2C12 cell lysate

Anti-DDX58 antibody - ab65588 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-DDX58 antibody (ab65588) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 4 : HEK293 Human embryonic kidney cell line Whole Cell LysateLane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-DDX58 antibody - ab111037 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
ab111037, at 3.75µg/ml, staining DDX58 in formalin-fixed, paraffin-embedded Human brain cortex tissue by Immunohistochemistry.

Anti-DDX58 antibody - ab112028 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Immunohistochemical expression of DDX58 in Formalin-Fixed, Paraffin-Embedded Human spleen using ab112028 at 3.75 µg/ml.

Anti-DDX58 antibody - ab93110 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-DDX58 antibody - ab155404 from Abcam

Host Rabbit
Clonality Polyclonal
All lanes : Anti-DDX58 antibody (ab155404) at 3 µg/mlLane 1 : 293 lysateLane 2 : 293 lysate with immunising peptideSecondaryGoat anti-rabbit Ig HRP

Anti-DDX58 antibody [35H2L48] - ab203562 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Monoclonal
Clone 35H2L48
Lane 1 : anti-Flag antibodyLane 2 : Anti-DDX58 antibody [35H2L48] (ab203562) at 4 µg/mlLane 1 : FLAG-tagged DDX58 transfected 293 lysateLane 2 : FLAG-tagged DDX58 transfected 293 lysate

Anti-DDX58 antibody [8D2] - ab140963 from Abcam

Prices $376.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 8D2
All lanes : Anti-DDX58 antibody [8D2] (ab140963) at 1/4000 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNALane 2 : HEK293T cells transfected with pCMV6-ENTRY DDX58 cDNALysates/proteins at 5 µg per lane.

Anti-DDX58 / RIG-1 / RIG-I Antibody (aa200-250) IHC-plus™ - LS-B8006 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-RIG-1 / RIG-I antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8006 dilution 5-10 ug/ml.

Anti-DDX58 / RIG-1 / RIG-I Antibody (clone 6C1) - LS-C174703 from LifeSpan Bioscience

Prices $350.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6C1
IHC of paraffin-embedded Human pancreas tissue using anti-DDX58 mouse monoclonal antibody.

Anti-DDX58 / RIG-1 / RIG-I Antibody IHC-plus™ - LS-B3768 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-DDX58 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3768 concentration 3.75 ug/ml.

Anti-DDX58 / RIG-1 / RIG-I Antibody (aa585-614) - LS-C97879 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of DDX58 in 293 cell line lysates (35 ug/lane). DDX58 (arrow) was detected using the purified antibody.

Anti-DDX58 / RIG-1 / RIG-I Antibody - LS-C122394 from LifeSpan Bioscience

Prices $425.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-DDX58 / RIG-1 / RIG-I Antibody (clone SS1A) - LS-C126229 from LifeSpan Bioscience

Prices $325.00
Sizes 25 µg
Host Rat
Clonality Monoclonal
Clone SS1A

Anti-DDX58 / RIG-1 / RIG-I Antibody (aa782-798) - LS-C144146 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-DDX58 / RIG-1 / RIG-I Antibody (aa185-203) - LS-C144145 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal

Anti-DDX58 / RIG-1 / RIG-I Antibody (aa894-925) - LS-C97877 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
24-hour post infection immunoblots of whole cell lysates from primary murine microglia cells (2x106) untreated (0) or exposed to vesicular stomatitis virus at a range of viral particle/cell ratios. Data courtesy of Dr. Samantha Furr, University of North Carolina at Charlotte.

Anti-DDX58 / RIG-1 / RIG-I Antibody (aa827-876) - LS-C110155 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
DDX58 antibody LS-C110155 Western blot of OVCAR-3 cell lysate.
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